OsCLT1, a CRT‐like transporter 1, is required for glutathione homeostasis and arsenic tolerance in rice

Yang, Jian; Gao, Ming; Hu, Han; Ding, Xiaomeng; Lin, Hongwei; Wang, Lei; Xu, Jiming; Mao, Chuanzao; Zhao, Fang‐Jie; Wu, Zhongchang

doi:10.1111/nph.13908

Cited by 86 publications

(55 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All transgenic rice lines were produced in the HJ2 or crd1 background. The rice plants were grown in a greenhouse and cultivated as previously described (Yang et al ., ). Arabidopsis plants were grown at 22°C with 16/8 h (light/dark) photoperiod at approximately 120 μmol m −2 sec −1 light (He et al ., ).…”

Section: Methodsmentioning

confidence: 97%

CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice

Zhu

Lin

et al. 2019

The Plant Journal

View full text Add to dashboard Cite

Crown root (CR) is the main component of the fibrous root system in cereal crops, but the molecular mechanism underlying CR development is still unclear. Here, we isolated the crown root defect 1 (crd1) mutant from ethyl methane sulfonate-mutated mutant library, which significantly inhibited CR development. The CRD1 was identified through genome resequencing and complementation analysis, which encodes an Xpo1 domain protein: the rice ortholog of Arabidopsis HASTY (HST) and human exportin-5 (XPO5). CRD1 is ubiquitously expressed, with the highest expression levels in the CR primordium at the stem base. CRD1 is a nucleocytoplasmic protein. The crd1 mutant contains significantly reduced miRNA levels in the cytoplasm and nucleus, suggesting that CRD1 is essential for maintaining normal miRNA levels in plant cells. The altered CR phenotype of crd1 was simulated by target mimicry of miR156, suggesting that this defect is due to the disruption of miR156 regulatory pathways. Our analysis of CRD1, the HST ortholog identified in monocots, expands our understanding of the molecular mechanisms underlying miRNA level and CR development.

show abstract

Section: Methodsmentioning

confidence: 97%

CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice

Zhu

Lin

et al. 2019

The Plant Journal

View full text Add to dashboard Cite

show abstract

“…It is important to note that an aquaporin channel of TIP class (TIP4;1) is suggested to be involved in As(III) uptake in P. vittata in a recent study by He et al (2016). Yang et al (2016) identified a transporter OsCLT1 [CRT (Chloroquine-Resistance Transporter)-Like transporter) in rice, which is localized to plastids. The mutant lines (Osclt1) had decreased PC2 levels as compared to WT upon exposure to As(V) or As(III) and showed a decrease in As concentration.…”

Section: The Transport and Subcellular Distribution Of Arsenic: The Imentioning

confidence: 99%

The Journey of Arsenic from Soil to Grain in Rice

et al. 2017

View full text Add to dashboard Cite

Arsenic (As) is a non-essential toxic metalloid whose elevated concentration in rice grains is a serious issue both for rice yield and quality, and for human health. The rice-As interactions, hence, have been studied extensively in past few decades. A deep understanding of factors influencing As uptake and transport from soil to grains can be helpful to tackle this issue so as to minimize grain As levels. As uptake at the root surface by rice plants depends on factors like iron plaque and radial oxygen loss. There is involvement of a number of transporters viz., phosphate transporters and aquaglyceroporins in the uptake and transport of different As species and in the movement to subcellular compartments. These processes are also affected by sulfur availability and consequently on the level of thiol (-SH)-containing As binding peptides viz., glutathione (GSH) and phytochelatins (PCs). Further, the role of phloem in As movement to the grains is also suggested. This review presents a detailed map of journey of As from soil to the grains. The implications for the utilization of available knowledge in minimizing As in rice grains are presented.

show abstract

“…MAN3 ( Endo‐β‐Mannanase 3 ) and ZAT6 ( Zinc‐Finger Transcription Factor 6 ) have been reported to regulate Cd tolerance through the GSH‐ and PC‐dependent pathway (Chen et al , , ). The rice CRT‐like transporter OsCLT1 mediates the export of γ ‐glutamylcysteine and glutathione from plastids to the cytoplasm for the synthesis of PCs; mutation of OsCLT1 results in hypersensitivity to both arsenic and Cd (Yang et al , ). Because of the demand on thiol compounds in Cd detoxification, exposure of maize roots to Cd markedly induced sulphate uptake by upregulating the expression of high‐affinity sulphate transporter genes (Nocito et al , , ), whereas tolerance of Cd is compromised under sulphur‐limiting conditions (Adhikari et al , ).…”

Section: Introductionmentioning

confidence: 99%

Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

et al. 2020

Self Cite

View full text Add to dashboard Cite

Summary How cadmium (Cd) tolerance in rice is regulated remains poorly understood. We used a forward genetic approach to investigate Cd tolerance in rice. Using a root elongation assay, we isolated a rice mutant with enhanced Cd tolerance, cadt1, from an ethyl methanesulphonate (EMS)‐mutagenized population of a widely grown Indica cultivar. The mutant accumulated more Cd in roots but not in shoots and grains. Using genomic resequencing and complementation, we identified OsCADT1 as the causal gene for the mutant phenotype, which encodes a putative serine hydroxymethyltransferase. OsCADT1 protein was localized to the nucleus and the OsCADT1 gene was expressed in both roots and shoots. OsCADT1 mutation resulted in higher sulphur and selenium accumulation in the shoots and grains. Selenate influx in cadt1 was 2.4 times that of the wild‐type. The mutant showed higher expression of the sulphate/selenate transporter gene OsSULTR1;1 and the sulphur‐deficiency‐inducible gene OsSDI1. Thiol compounds including cysteine, glutathione and phytochelatins were significantly increased in the mutant, underlying its increased Cd tolerance. Growth and grain biomass were little affected. The results suggest that OsCADT1 acts as a negative regulator of sulphate/selenate uptake and assimilation. OsCADT1 mutation increases Cd tolerance and enriches selenium in rice grains, providing a novel solution for selenium biofortification.

show abstract

OsCLT1, a CRT‐like transporter 1, is required for glutathione homeostasis and arsenic tolerance in rice

Cited by 86 publications

References 46 publications

CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice

CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice

The Journey of Arsenic from Soil to Grain in Rice

Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

Contact Info

Product

Resources

About