OsExo70B1 Positively Regulates Disease Resistance to Magnaporthe oryzae in Rice

Hou, Hongna; Fang, Junyong; Liang, Jiahui; Diao, Zhijuan; Wang, Wei; Yang, Dewei; Li, Shengping; Tang, Dingzhong

doi:10.3390/ijms21197049

Cited by 23 publications

(20 citation statements)

References 58 publications

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“…EXO70 isoforms also function in plant immunity. For instance, OsEXO70B1 acts as an essential regulator in rice immunity by interacting with the receptor‐like kinase OsCERK1, an essential component for chitin reception (Hou et al ., 2020). OsEXO70F3/F2 specifically forms a complex with the Magnaporthe oryzae effector AVR‐Pii and is necessary for NLR protein Pii‐dependent resistance (Fujisaki et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls

Guo

Zhang

et al. 2022

New Phytologist

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Summary The planthopper resistance gene Bph6 encodes a protein that interacts with OsEXO70E1. EXO70 forms a family of paralogues in rice. We hypothesized that the EXO70‐dependent trafficking pathway affects the excretion of resistance‐related proteins, thus impacting plant resistance to planthoppers. Here, we further explored the function of EXO70 members in rice resistance against planthoppers. We used the yeast two‐hybrid and co‐immunoprecipitation assays to identify proteins that play roles in Bph6‐mediated planthopper resistance. The functions of the identified proteins were characterized via gene transformation, plant resistance evaluation, insect performance, cell excretion observation and cell wall component analyses. We discovered that another EXO70 member, OsEXO70H3, interacted with BPH6 and functioned in cell excretion and in Bph6‐mediated planthopper resistance. We further found that OsEXO70H3 interacted with an S‐adenosylmethionine synthetase‐like protein (SAMSL) and increased the delivery of SAMSL outside the cells. The functional impairment of OsEXO70H3 and SAMSL reduced the lignin content and the planthopper resistance level of rice plants. Our results suggest that OsEXO70H3 may recruit SAMSL and help its excretion to the apoplast where it may be involved in lignin deposition in cell walls, thus contributing to rice resistance to planthoppers.

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Section: Introductionmentioning

confidence: 99%

Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls

Guo

Zhang

et al. 2022

New Phytologist

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“…Like FLS2, Rps8 LRR-RK requires Exo70FX12, a member of the Poales-specific Exo70FX clade. The specialized role of Exo70FX12 contrasts with the conserved requirement of Exo70B family members in maintaining diverse receptor kinase abundance in the plasma membrane (Hou et al, 2020; Wang et al, 2020). As the Exo70B clade is conserved in all angiosperms (Cvrčková et al, 2012) and expressed in all cell layers of barley leaves, this indicates that Exo70FX12 role in Rps8 LRR-RK signaling is distinct.…”

Section: Discussionmentioning

confidence: 96%

“…EXO70B1 and EXO70B2 were found to directly interact with FLS2, as well as hetero-oligomerize (Wang et al, 2020). This role in membrane receptor signaling is likely conserved in monocots, at least for chitin recognition, as mutants in OsExo70B1 have increased susceptibility to rice blast ( Magnaporthe oryzae ) and OsExo70B1 interacts directly with the chitin receptor CERK1 (Hou et al, 2020). Like FLS2, Rps8 LRR-RK requires Exo70FX12, a member of the Poales-specific Exo70FX clade.…”

Section: Discussionmentioning

confidence: 99%

A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley

Holden

Bergum

Green

et al. 2021

Preprint

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In the evolution of land plants, the plant immune system has experienced expansion in immune receptor and signaling pathways. Lineage-specific expansions have been observed in diverse gene families that are potentially involved in immunity, but lack causal association. Here, we show that Rps8-mediated resistance in barley to the fungal pathogen Puccinia striiformis f. sp. tritici (wheat stripe rust) is conferred by a genetic module: LRR-RK and Exo70FX12, which are together necessary and sufficient. The Rps8 LRR-RK is the ortholog of rice extracellular immune receptor Xa21 and Exo70FX12 is a member of the Poales-specific Exo70FX clade. The Exo70FX clade emerged after the divergence of the Bromeliaceae and Poaceae, and comprises from 2 to 75 members in sequenced grasses. These results demonstrate the requirement of a lineage-specific Exo70FX12 in Rps8 LRR-RK immunity and suggest that the Exo70FX clade may have evolved a specialized role in receptor kinase signaling.

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“…M. oryzae inoculation was performed as described previously (Hou et al, 2020). Briefly, 2‐week‐old rice seedlings were sprayed with M. oryzae spore suspensions at a concentration of 10 5 spores/ml in 0.02% Tween‐20 solution.…”

Section: Methodsmentioning

confidence: 99%

“…M. oryzae inoculation was performed as described previously (Hou et al, 2020). Briefly, 2-week-old rice seedlings were sprayed with M.…”

Section: Rice Blast Fungus Inoculation Assaymentioning

confidence: 99%

The 14‐3‐3 protein GF14c positively regulates immunity by modulating the protein homoeostasis of the GRAS protein OsSCL7 in rice

Diao

Yang

et al. 2022

Plant Cell & Environment

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Various types of transcription factors have been reported to be involved in plantpathogen interactions by regulating defence-related genes. GRAS proteins, plantspecific transcription factors, have been shown to play essential roles in plant growth, development and stress responses. By performing a transcriptome study on rice early defence responses to Magnaporthe oryzae, we identified a GRAS protein, OsSCL7, which was induced by M. oryzae infection. We characterized the function of OsSCL7 in rice disease resistance. OsSCL7 was upregulated upon exposure to M. oryzae and pathogen-associated molecular pattern treatments, and knocking out OsSCL7 resulted in decreased disease resistance of rice to M. oryzae. In contrast, overexpression of OsSCL7 could improve rice disease resistance to M. oryzae.OsSCL7 was mainly localized in the nucleus and showed transcriptional activity.OsSCL7 can interact with GF14c, a 14-3-3 protein, and loss-of-function GF14c leads to enhanced susceptibility to M. oryzae. Additionally, OsSCL7 protein levels were reduced in the gf14c mutant and knocking out OsSCL7 affected the expression of a series of defence-related genes. Taken together, these findings uncover the important roles of OsSCL7 and GF14c in plant immunity and a potential mechanism by which plants fine-tune immunity by regulating the protein stability of a GRAS protein via a 14-3-3 protein.

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OsExo70B1 Positively Regulates Disease Resistance to Magnaporthe oryzae in Rice

Cited by 23 publications

References 58 publications

Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls

Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls

A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley

The 14‐3‐3 protein GF14c positively regulates immunity by modulating the protein homoeostasis of the GRAS protein OsSCL7 in rice

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