Osmotic hemolysis and fragility A new model based on membrane disruption, and a potential clinical test

Akeson, Stephen P.; Mel, Howard C.

doi:10.1016/0304-4165(82)90220-3

Cited by 16 publications

(9 citation statements)

References 32 publications

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“…As was noted in Results, in confirmation of previous work (Akeson and Mel, 1982), the long-term ghost resistivity remained higher than that of the lysing medium. The likely explanation for this result was found in the ghost's residual hemoglobin: as much as 7% of the cell's original hemoglobin can be permanently trapped in the "pink" ghosts that are formed in these intermediate osmolalities (Dodge et al, 1963).…”

Section: Kinetics Of Resistivity Change During Osmotic Hemolysissupporting

confidence: 92%

“…The resulting kinetics of resistivity change, shown in Fig. 4, were consistent with previously published models of hemolysis (Akeson and Mel, 1982). The intact cells (cytoplasms) slowly increased in resistivity.…”

Section: Kinetics Of Resistivity Change During Osmotic Hemolysissupporting

confidence: 88%

“…The Laplace solution was then applied to determine the changes in cytoplasmic resistivity of ghosts and intact cells during the first 5 min after suspension in hypotonic media, and the results interpreted in terms of a recently proposed model of hemolysis (Akeson and Mel, 1982). We also demonstrated a new phenomenon in the electronic sizing of ghosts, where interaction with sufficiently high electric fields rendered ghosts completely transparent to the sizing process, so that the ghosts effectively "disappeared.…”

Section: Introductionmentioning

confidence: 79%

“…Now, turning to the process of ghost formation, the kinetics of change in resistivity were governed by the exchange of total cell contents, as opposed to a selective K+ loss. As was recently emphasized (Akeson and Mel, 1982), the resistivity of the intact cell is substantially higher than that of the medium. Consequently, the exchange of cytoplasm with the medium during ghost formation actually led to a reduction of internal resistivity.…”

Section: Kinetics Of Resistivity Change During Osmotic Hemolysismentioning

confidence: 90%

“…Open symbols (o, 0), low electric field strengths; solid symbols (e, U), high field strengths. (Top two curves are adapted from Akeson and Mel, 1982. ) ghosts are spheres with shape factors of 1.5 in the nonconducting state, even though the apparent volume of the early ghosts was significantly smaller in the earliest time points (channel 42.5 compared with 47.5 for the intact cells).…”

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confidence: 99%

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Erythrocyte and ghost cytoplasmic resistivity and voltage-dependent apparent size

Akeson¹,

Mel²

1983

Biophysical Journal

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Particle resistivity is explicitly included in the equations relating volume to voltage pulse, in electronic cell sizing or resistive pulse spectroscopy (RPS). It has long been known that in high electric fields cell resistivity decreases as the membrane undergoes dielectric breakdown. At sufficiently high electric field strengths, well past dielectric breakdown, the red cell membrane becomes electrically transparent, or nearly so, and apparent cell size becomes essentially a function of the cytoplasmic resistivity. Electronic cell sizing is traditionally carried out at low electric field strengths, and corrections made for the influence of cell shape by use of the Laplace equation. We find the Laplace solution to be still applicable at very high electric field strengths for purposes of calculating specific cytoplasmic resistivity from RPS measurements. Our value for discocytes, 220 omega X cm, is in good agreement with published results obtained by other researchers using other techniques. We have also applied these same procedures to determine the time course of voltage-dependent resistivity changes in ghosts and intact spherocytes, during the first 5 min after suspension in hypotonic medium. We believe these to be the first explicit calculations of particle specific resistivity from post-dielectric-breakdown apparent size, using traditional electronic sizing techniques.

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Section: Kinetics Of Resistivity Change During Osmotic Hemolysissupporting

confidence: 92%

Section: Kinetics Of Resistivity Change During Osmotic Hemolysissupporting

confidence: 88%

Section: Introductionmentioning

confidence: 79%

Section: Kinetics Of Resistivity Change During Osmotic Hemolysismentioning

confidence: 90%

mentioning

confidence: 99%

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