Osmotic regulation ofmyo-inositol uptake in primary astrocyte cultures

Isaacks, R. E.; Bender, Alex S.; Kim, Chang Y.; Prieto, Nicole M.; Norenberg, Michael D.

doi:10.1007/bf00971582

Cited by 115 publications

(83 citation statements)

References 44 publications

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“…It is a major osmolyte and also serves as the precursor to phosphatidylinositol. mI has been used as a glial cell marker (13). The decrease in mI/Cho ratios between 32 and 41 wk found in the present study was described before by Kreis et al (14), who suggested that the concentration of mI was associated with postnatal life rather than with gestational age.…”

Section: Discussionsupporting

confidence: 88%

“…Elevated levels of lactate (Lac) in the brain of asphyxiated neonates, even months after the hypoxic-ischemic insult, have been shown to be predictive of neurodevelopmental delay (7,8,10,12). Myo-inositol (mI), which is one of the osmoregulators of the brain, can be found in astrocytes and is considered a glial cell marker (13). It increases in the early phase after hypoxia-ischemia and decreases during the perinatal period (14,15).…”

Section: H-mrs)mentioning

confidence: 99%

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Cerebral Structure and Metabolism and Long-Term Outcome in Small-for-Gestational-Age Preterm Neonates

et al. 2004

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In the present study, we compared brain development and metabolism of small-for-gestational-age (SGA) and appropriate-forgestational-age (AGA) infants using proton magnetic resonance spectroscopy ( 1 H-MRS). We tested the hypothesis that intrauterine growth retardation caused by placental insufficiency is associated with changes in cerebral metabolism and is followed by an adverse neurodevelopmental outcome at the age of 2 y. Twenty-six AGA and 14 SGA (birth weight ϽP 2.3) preterm infants with no major ultrasound abnormalities were enrolled prospectively. At 32 and 41 wk postmenstrual age, 1 H-MRS and magnetic resonance imaging were performed. For 1 H-MRS, a volume of interest was placed in the basal ganglia and in the periventricular white matter. Using echo times of 31 and 144 ms N-acetylaspartate/choline (NAA/Cho), lactate/Cho, myo-inositol/Cho (mI/Cho), and glutamate-glutamine-␥-aminobutyric acid/Cho (Glx/Cho) ratios were compared between AGA and SGA groups. Griffiths' developmental quotient (DQ) values were assessed at 24 mo corrected age. Griffiths' DQ (AGA, 104 Ϯ 10; SGA, 99 Ϯ 9) and brain development assessed using magnetic resonance imaging showed no significant differences between both AGA and SGA groups, and NAA/Cho, Lac/Cho, mI/ Cho, and Glx/Cho ratios were not significantly different between the groups. NAA/Cho ratios increased from 32 to 41 wk, whereas mI/Cho ratios decreased in both groups. Preterm infants who are small for gestational age (SGA) as a result of placental insufficiency are at increased risk for adverse neurodevelopmental outcome in comparison with agematched appropriate-for-gestational-age (AGA) neonates (1-4). Placental insufficiency can be demonstrated in these pregnancies using Doppler ultrasonography of the umbilical artery (5). Most of these pregnancies are terminated by cesarean section because of severe fetal compromise with impending hypoxia-ischemia (6).During the past decade, it was demonstrated that perinatal hypoxia-ischemia may have long-lasting effects on cerebral metabolism as has been demonstrated using in vivo cerebral proton magnetic resonance spectroscopy ( 1 H-MRS) (7,8). A decrease of the N-acetylaspartate/choline (NAA/Cho) ratio in asphyxiated full-term neonates predicts an adverse neurodevelopmental outcome (7,9 -11). Elevated levels of lactate (Lac) in the brain of asphyxiated neonates, even months after the hypoxic-ischemic insult, have been shown to be predictive of neurodevelopmental delay (7,8,10,12

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Section: Discussionsupporting

confidence: 88%

Section: H-mrs)mentioning

confidence: 99%

Cerebral Structure and Metabolism and Long-Term Outcome in Small-for-Gestational-Age Preterm Neonates

et al. 2004

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“…mIns is thought to be a glial marker and an organic osmolyte that plays a major role in the osmoregulation of astrocytes. 38 We interpreted our findings as local changes reflecting the effect of Glu. This positive relation between mIns/Cr with the VAS and FIQ could be used to monitor therapy responses, and further studies are needed to confirm this relationship.…”

Section: Discussionmentioning

confidence: 80%

Metabolic Abnormalities in Pain-Processing Regions of Patients with Fibromyalgia: A 3T MR Spectroscopy Study

Feraco¹,

Bacci²,

Pedrabissi³

et al. 2011

AJNR Am J Neuroradiol

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“…It has also been proposed that reductions in brain concentrations of myo-inositol, an important organic osmolyte [13], which is localized predominantly in glia [5], are implicated in the pathogenesis of ammonia-induced brain edema [9]. Indeed, both biochemical and spectroscopic studies in experimental liver failure [9] and [11] as well as in ammonia-treated cultured astrocytes [23] provide evidence for decreased brain myo-inositol concentrations concomitant with cell swelling.…”

Section: Discussionmentioning

confidence: 99%

Selective alterations of brain osmolytes in acute liver failure: protective effect of mild hypothermia

et al. 2004

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Zwingmann, C. et al., 2004. Selective alterations of brain osmolytes in acute liver failure: protective effect of mild hypothermia. Brain Research, 999(1), ABSTRACTThe principal cause of mortality in patients with acute liver failure (ALF) is brain herniation resulting from intracranial hypertension caused by a progressive increase of brain water. In the present study, ex vivo highresolution 1 H-NMR spectroscopy was used to investigate the effects of ALF, with or without superimposed hypothermia, on brain organic osmolyte concentrations in relation to the severity of encephalopathy and brain edema in rats with ALF due to hepatic devascularization. In normothermic ALF rats, glutamine concentrations in frontal cortex increased more than fourfold at precoma stages, i.e. prior to the onset of severe encephalopathy, but showed no further increase at coma stages. In parallel with glutamine accumulation, the brain organic osmolytes myo-inositol and taurine were significantly decreased in frontal cortex to 63% and 67% of control values, respectively, at precoma stages (p<0.01), and to 58% and 67%, respectively, at coma stages of encephalopathy (p<0.01). Hypothermia, which prevented brain edema and encephalopathy in ALF rats, significantly attenuated the depletion of myo-inositol and taurine. Brain glutamine concentrations, on the other hand, did not respond to hypothermia. These findings demonstrate that experimental ALF results in selective changes in brain organic osmolytes as a function of the degree of encephalopathy which are associated with brain edema, and provides a further rationale for the continued use of hypothermia in the management of this condition.

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Osmotic regulation ofmyo-inositol uptake in primary astrocyte cultures

Cited by 115 publications

References 44 publications

Cerebral Structure and Metabolism and Long-Term Outcome in Small-for-Gestational-Age Preterm Neonates

Cerebral Structure and Metabolism and Long-Term Outcome in Small-for-Gestational-Age Preterm Neonates

Metabolic Abnormalities in Pain-Processing Regions of Patients with Fibromyalgia: A 3T MR Spectroscopy Study

Selective alterations of brain osmolytes in acute liver failure: protective effect of mild hypothermia

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