Osmotic stabilizer-coupled suppression of NDR defects is dependent on the calcium–calcineurin signaling cascade in Aspergillus nidulans

Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum

“…In filamentous fungi, hyphal branching is one of the essential phenotypes of polarized growth regulated by Ca 2+ (Silverman-Gavrila and Lew, 2003). In Aspergillus nidulans, the osmotic stabilizer-coupled suppression of the defects in the NDR (nuclear Dbf2-related) protein kinase signaling pathway is dependent on calcium-calcineurin signaling and might therefore regulate polarized cell growth (Gao et al, 2011). Similar phenomena were reported in Nox-silenced strains of G. lucidum in which lower Ca 2+ levels caused more hyphal branching (Mu et al, 2014).…”

“…After Fluo-3 AM enters a cell, this dye is readily hydrolyzed into a Ca 2+ -binding form, Fluo-3, by an endogenous esterase (Gao et al, 2011). Subsequently, the Fluo-3-labeled cells were examined using a Zeiss Axio Imager.A1 Fluorescence Microscope; the cells were excited at 488 nm with an Ar laser, and fluorescence was detected using a 505-530 nm band pass filter.…”

Hyphal Morphology and Elongation Alterations in <i>Aspergillus nidulans</i> Provoked by the Diterpene Kaurenoic Acid

“…Aspergillus nidulans strain FGSC A26 (biA1, veA1) was maintained according Vanzela and Said [12] and grown at 30˚C for seven days before inoculating them on the culture medium (MM) containing nitrate salts and trace elements prepared according to Käfer [13] supplemented with 0.5% glucose and 0.02 µg/mL biotin; 1.8% agar was added and the pH was adjusted to 6.0.…”

CYP2J2-Derived Epoxyeicosatrienoic Acids Suppress Endoplasmic Reticulum Stress in Heart Failure

“…H9C2 cells were cultured and pretreated with 14,15-EET 1 hour before treatment with ISO (10 mM) or AngII (100 nM). Twenty-four hours later, the intracellular Ca 21 concentration was measured using a Fluo-3 AM kit from Invitrogen according to the supplier's instructions, as previously described (Gao et al, 2011). Relative intracellular Ca 21 was expressed as a percentage of the control.…”