Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea

Hatmi, Saloua; Trotel‐Aziz, Patricia; Villaume, Sandra; Couderchet, Michel; Clément, Christophe; Aziz, Aziz

doi:10.1093/jxb/ert351

Cited by 49 publications

(47 citation statements)

References 45 publications

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“…PA oxidation has been associated with stress-induced phenylpropanoid metabolism in cell cultures of the grape vine (Vitis vinifera L. -Hatmi et al 2014). PA oxidation releases H2O2, which promotes cell-wall stiffening through formation of covalent cross links between cell wall components (Pottosin et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Muilu‐Mäkelä¹,

Vuosku²,

Saarinen³

et al. 2017

iForest

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(1) Polyamines (PA) are ubiquitous polycations known to be involved in several phases of plant development as well as in tolerance to abiotic stresses. Phenols are complex secondary metabolites produced via the phenylpropanoid pathway that contain, e.g., cell wall compounds and antioxidants. Phenols are known to enhance chilling tolerance of plants. PA and phenolic pathways are connected via conjugation. In boreal coniferous forests spring frost has been considered to have severe effects on the survival of tree seedlings. Such effects are likely to increase in the future. The present study focuses on the role of PA and phenylpropanoid syntheses in the coping strategies of Scots pine exposed to cold temperatures during the vulnerable early seedling phase in late spring and early summer. We found that spring frost affects the expression of genes regulating PA metabolism and phenylpropanoid synthesis differently in above and below ground parts of the seedlings, whereas PA or phenol contents in tissues were not affected. The results suggest that Scots pine seedlings may not have time to develop metabolite level responses during a short period of freezing stress and, therefore, the originally different PA levels, especially in roots, may influence the tolerance of Scots pine seedlings to spring frost.

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Section: Discussionmentioning

confidence: 99%

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Muilu‐Mäkelä¹,

Vuosku²,

Saarinen³

et al. 2017

iForest

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“…According to Deluc et al (2011), water deficit induced the levels of tpiceid in Cabernet Sauvignon berries but not in Chardonnay. Hatmi et al (2014) found that osmotic stress induced by PEG and sucrose increased the accumulation of tresveratrol and e-viniferin in the leaves of V. vinifera cv. Chardonnay.…”

Section: Biotic and Abiotic Stresses As Regulators Of Stilbene Biosynmentioning

confidence: 97%

Regulation of stilbene biosynthesis in plants

Dubrovina

Kiselev

2017

Planta

140

126

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Main conclusion This review analyzes the advances in understanding the natural signaling pathways and environmental factors regulating stilbene biosynthesis. We also discuss the studies reporting on stilbene content and repertoire in plants.Stilbenes, including the most-studied stilbene resveratrol, are a family of phenolic plant secondary metabolites that have been the subject of intensive research due to their valuable pharmaceutical effects and contribution to plant disease resistance. Understanding the natural mechanisms regulating stilbene biosynthesis in plants could be useful for both the development of new plant protection strategies and for commercial stilbene production. In this review, we focus on the environmental factors and cell signaling pathways regulating stilbene biosynthesis in plants and make a comparison with the regulation of flavonoid biosynthesis. This review also analyzes the recent data on stilbene biosynthetic genes and summarizes the available studies reporting on both stilbene content and stilbene composition in different plant families.

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“…In proembryogenic cells (i.e., the cells not yet induced to maturation), the content of secondary metabolites is generally low (Vuosku et al 2012). However, osmotic stress has been found to induce secondary metabolism also in the proembryogenic cell cultures (Cvikrová et al 2008;Hatmi et al 2014) and can be related to, i.e., cell wall stiffening (Boudet 2007). Phenylalanine ammonia lyase (PAL) links primary and secondary metabolism by catalyzing the conversion of L-phenylalanine to cinnamic acid, the initial substrate of phenylpropanoid metabolism (Butland et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The content of hydroxycinnamic acid was decreased by PAL inhibitor in alfalfa (Medicago sativa) suspension cultures, which influenced the balance of free and conjugated PA contents and stimulated cell division activity (Cvikrová et al 1999). Moreover, in grape vine (Vitis vinifera L.) subjected to osmotic stress, PA homeostasis was recently linked with parallel accumulation of main stilbenes and up-regulation of gene transcripts including stilbene synthases (STS) (Hatmi et al 2014). psSTS of Scots pine has been characterized as a dihydropinosylvinsynthase (Fliegmann et al 1992), and psSTS catalyzes biosynthesis of the stilbene backbone from malnoyl-CoA and one CoA-ester of cinnamic acid derivative (Chong et al 2009) (see the figure Online Resource 2).…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress affects polyamine homeostasis and phenolic content in proembryogenic liquid cell cultures of Scots pine

Muilu‐Mäkelä

Vuosku

Hamberg

et al. 2015

Plant Cell Tiss Organ Cult

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Polyamines (PAs) are ubiquitous polycations involved in many physiological processes in plants, including somatic embryogenesis, cellular growth and stress reactions. In the present study, we focus on the consequences in PA metabolism caused by polyethylene glycol (PEG) in proembryogenic Scots pine (Pinus sylvestris L.) liquid cultures. The growth and viability of the cell masses and changes in PA concentrations and phenolic secondary metabolites were investigated under control, 5 and 10 % PEG treatments. The effect of osmotic stress responses was investigated at the gene expression level including stress, cell division, programmed cell death and PA-related genes and PA metabolites. Moreover, the expression of ethylene and proline biosynthesis genes and phenylalanine ammonia lyase and stilbene synthase (psSTS) was analyzed. Under osmotic stress conditions, we found a consistent pattern of endogenous PAs in Scots pine proembryogenic cells. However, accumulation of free spermine (Spm) and methyl putrescine under osmotic stress might indicate their specific role in stress protection. Expression of polyamine oxidase was down-regulated under osmotic stress, suggesting the role of PA catabolism in regulation of Spm levels. Scots pine proliferating proembryogenic cells are in a developmentally undifferentiated stage where the content of secondary metabolites is generally low. However, in the present study the total content of phenolic compounds increased but the biosynthesis of phenylpropanoids and stilbenes, generally considered as stress-protective molecules, was not affected by osmotic stress.

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Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea

Cited by 49 publications

References 45 publications

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Regulation of stilbene biosynthesis in plants

Osmotic stress affects polyamine homeostasis and phenolic content in proembryogenic liquid cell cultures of Scots pine

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