Osmotic versus nutritional effects when rooting in vitro asparagus minicrowns on high sucrose media

Conner, A. J.; Falloon, Peter G.

doi:10.1016/0168-9452(93)90175-y

Cited by 22 publications

(5 citation statements)

References 12 publications

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“…Moreover, the effect of these osmotic agents depends on the temperature, genotype, and culture conditions, as suggested by Marino et al (2010). Mannitol has often been found to have deleterious effects on the quality of stored cultures (Conner and Falloon 1993;Cordeiro et al 2014), in contrast to our findings. In our experiments, effective storage was achieved by incorporation of mannitol at low concentrations into the medium, whereas higher concentrations of osmoticum were deleterious to in vitro-stored cultures of A. racemosus.…”

Section: Resultscontrasting

confidence: 84%

In vitro approaches for conservation of Asparagus racemosus Willd.

Thakur

Tiwari

Jadhav

2015

In Vitro Cell.Dev.Biol.-Plant

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Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in India. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species. The plants are being overharvested, so this species faces a real danger of becoming vulnerable in its natural habitat. Ex situ conservation using in vitro tools is a possible solution to this problem. Ex situ conservation of plants involving in vitro tools has been initiated through axillary branching using nodal explants. Studies on in vitro storage under slow-growth conditions were carried out to develop an efficient protocol for conservation of A. racemosus germplasm. In vitro shoot cultures generally require a 4-wk subculture onto fresh medium when grown at 25±2°C under a 16-h photoperiod. In this research, the use of mannitol or sorbitol as an osmoticum and reduction of sucrose to 1.5% (w/v) in half-strength MS medium led to maintenance of the cultures for 6 mo at 25±2°C with no subculture. Surviving shoots from the slow-growth cultures could be regenerated with 100% efficiency, indicating that the subculture interval was successfully extended by this method. Temperature and medium modification both had significant effects on the growth of stored shoots, and the two factors showed significant interaction. In experiments designed to test encapsulation as a storage method, micropropagated shoot clusters encapsulated in calcium alginate beads were successfully stored up to 75 d at 25±2°C under a 16-h photoperiod. Stored shoots from both storage methods were subsequently recovered and multiplied on MS medium with 3% sucrose and 1.11 μM benzylaminopurine at 25 ± 2°C. Welldeveloped shoots were rooted and acclimatized successfully.

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Section: Resultscontrasting

confidence: 84%

In vitro approaches for conservation of Asparagus racemosus Willd.

Thakur

Tiwari

Jadhav

2015

In Vitro Cell.Dev.Biol.-Plant

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“…In plant tissue culture medium, sugars (sucrose in particular) are the most predominant components, and play a critical role as osmoticums as well as carbon sources (Sarkissian and Fowler 1986;Conner and Falloon 1993). Supplementation of sucrose (29-88 mM) to culture media under both ambient and elevated CO 2 conditions led to a reduction in shoot and root performance of in vitro plantlets (Suriyan et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture

Jian-ping

Guan

2011

Plant Cell Tiss Organ Cult

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The influence of semi-continuous culture on root growth and production of saikosaponins was investigated using root cultures of Bupleurum chinense DC. When fresh medium, consisting of half-strength of Murashige and Skoog liquid medium and 5% sucrose, was added at day 30, numerous new young roots developed and grew. Also, an increase in root weight and the total amount of saikosaponins was observed at day 60. Both the concentration and time of adding sucrose to the culture medium had significant effects on root growth and production of total saikosaponins. The sucrose concentration was controlled by multiple additions of fresh sucrose at different time points during root culture. Compared to other reported methods, by which sucrose concentration was controlled in culture, sequential increases of 1% sucrose at days 5, 10, 15, and 20 promoted both root growth and the production of saikosaponins.

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“…Changes were based on the conservation medium used to store in vitro potato tubers (Lizarraga et al 1989) where sucrose in the medium was supplemented with mannitol. However, since mannitol is reported to inhibit growth of micropropagated asparagus plants (Conner & Falloon 1993), the medium contained sucrose plus sorbitol which, like mannitol, is also a sugar alcohol. The conservation medium consisted of M & S salts with the addition of 3% sucrose, 4% sorbitol, 0.4 mg/litre thiamine, 200 mg/litre glutamine, 100 mg/litre inositol, 1.0 mg/litre ancymidol, 0.8% Gibco bacteriological agar with the pH adjusted to 5.8 before autoclaving.…”

Section: Long-term Storage Mediummentioning

confidence: 99%

In vitro long‐term storage of asparagus

Fletcher

1994

New Zealand Journal of Crop and Horticultural Science

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To assist with commercial production of asparagus (Asparagus officinalis L.) clones, a system for in vitro, long-term cold storage of high health asparagus germplasm was developed. In vitro mini-crowns, with and without storage roots, were stored on a medium consisting of Murashige and Skoog salts with the addition of 3% sucrose, 4% sorbitol, 0.4 mg/litre thiamine, 200 mg/litre glutamine, 100 mg/litre inositol, 1.0 mg/litre ancymidol, and 0.8% agar. The cultures were incubated at 6°C with a photoperiod of 16 h (light level of 70-90 µmol/m 2 per s). The role of sorbitol was examined, as was the value of photoperiod. After 16 months of storage, the viability and reculturing ability of the in vitro cultures was assessed. To date, the cultures have survived 2 years of storage with 100% success in regrowth to their original growth form in culture and on transfer to glasshouse conditions.

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Osmotic versus nutritional effects when rooting in vitro asparagus minicrowns on high sucrose media

Cited by 22 publications

References 12 publications

In vitro approaches for conservation of Asparagus racemosus Willd.

In vitro approaches for conservation of Asparagus racemosus Willd.

Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture

In vitro long‐term storage of asparagus

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