Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture

Jian-ping, Hao; Guan, Qingmei

doi:10.1007/s11240-011-0012-x

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…However, when examining the different levels of sucrose within each type of closure, we found that sucrose-free medium associated with PTFE membranes had the highest levels of the active principle (20E), which was a significant accumulation (Table 3). These results differ from those described by Hao and Guan (2012), where the addition of sugars increased the accumulation of secondary metabolites. However, these authors worked with adventitious roots whereas we worked with the whole plant.…”

Section: Conditions That Enhance Photoautotrophy Induced Greater Levecontrasting

confidence: 99%

Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]

Iarema

Cruz

Saldanha

et al. 2012

Plant Cell Tiss Organ Cult

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Pfaffia glomerata (Spreng.) Pedersen is a medicinal species of great interest because it produces the phytoecdysteroid 20-hydroxyecdysone (20E). Generally, because of atypical growing conditions, in vitro propagated plants function less efficiently as autotrophs and have poorly developed morphological structures. This study analyzed the autotrophic potential of P. glomerata propagated in vitro and evaluated the influence that this has on 20E biosynthesis. Physiological and structural parameters of plants subjected to heterotrophic, photomixotrophic and photoautotrophic growth conditions were evaluated. Levels of 20E were measured by HPLC. Plants were acclimatized in a mixture of soil, sand and substrate, in a greenhouse. Conditions that provided higher carbon input led to an increase in plant growth, and the presence of sucrose was critical, in closure systems without a gas permeable membrane, for normal anatomical development of the micropropagated plants. The absence of sucrose increased photosynthesis and conditions that enhanced photoautotrophy induced greater levels of 20E. The increase of 20E levels by the photoautotrophic system offers new prospects for increasing the commercial production of this species, and for studies that could elucidate the biosynthetic pathway of phytoecdysteroids in plants.

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Section: Conditions That Enhance Photoautotrophy Induced Greater Levecontrasting

confidence: 99%

Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]

Iarema

Cruz

Saldanha

et al. 2012

Plant Cell Tiss Organ Cult

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“…Instead of using the initial sucrose concentration, Hao and Guan (2012) showed that controlling sucrose concentration by multiple addition of fresh sucrose at different time points can enhance the adventitious roots proliferation and saikosaponin production of Bupleurum chinense.…”

Section: Sucrose Concentrationmentioning

confidence: 99%

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

Saiman

Mustafa

Schulte³

et al. 2012

Plant Cell Tiss Organ Cult

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Gynura procumbens is a medicinal plant used in South East Asia to treat various ailments such as rash, hemorrhoids, inflammation, and diabetes. In order to develop a large-scale culture system for G. procumbens biomass containing bioactive compounds, adventitious root cultures were initiated from leaf explants. Murashige and Skoog (MS) media containing different compositions of indole-3-butyric acid (IBA), 1-naphthalene-acetic acid (NAA), and combinations of both plant growth regulators (PGRs) were evaluated for root induction. A combination of 3 mg/l NAA ? 1 mg/l IBA gave the highest root induction (48%) as compared to other PGRs treatments after 9 weeks of incubation period. Subsequently, the adventitious roots were established in liquid culture containing MS medium and the combination of 3 mg/l NAA ? 1 mg/l IBA. A study on the medium strength, sucrose concentration, pH, and light versus dark was conducted to optimize the in vitro culture conditions. The results showed that differences in MS medium strength from half to double strength, and light or dark condition did not significantly affect the biomass production, while the initial medium pH of 5.5 and 2% w/v sucrose concentration were most suitable for the root culture growth. Nuclear magnetic resonance (NMR) spectroscopy was performed to characterize the metabolite content in the root cultures of G. procumbens. Among the elucidated metabolites were some phenylpropanoids identified as caffeic acid, chlorogenic acid, and 3,5-di-O-caffeoylquinic acid which might be the bioactive compounds associated to the folk use of this plant.

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“…Taxol (plaxitaxol): The bark of the taxus tree (Taxus wallichiana) produces a complex diterpene alkaloid which is a potent anticancer agent because of its unique mode of action on Artemisia annua Artemisinin Callus [66] Artemisia Annua Artemisinin Callus [67] Aspidosperma ramiflorum Ramiflorin Callus [68] Azadirachta indica Azadirachtin Suspension [69] Azadirachta indica Azadirachtin Anther culture [70] Azadirachta indica Azadirachtin Suspension [71] Azadirachta indica Azadirachtin Hairy root [72] Azadirachta indica Azadirachtin Hairy root [73] Brucea javanica Cathin Suspension [74] Bupleurum falcatum Saikosaponins Root [75] Bupleurum chinensis Saikosaponins Root [76] Camellia chinensis Flavones Callus [77] Capsicum annum Capsiacin Callus [78] Capsicum chinense Capsiacin Suspension [79] Capsicum chinense Capsiacin Cotyledon [80] Capsicum chinense Capsiacin Suspension [81] Cassia acutifolia Anthraquinones Suspension [82] Aloe barbadensis Anthraquinone Callus [83] Morinda citrifola Anthraquinone Suspension [84] ( Table 1). Continued.…”

Section: Production Of Bioactive Natural Products Using Tissue Culturmentioning

confidence: 99%

Bioactive Natural Products from Plants and Biotechnological Approaches for their Production

Tripathi

Sapre

Mishra

et al. 2016

Int. J. Biotech. Well. Indus.

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Bioactive natural products are economically important as drugs, fragrances, pigments, food additives and pesticides. The biotechnological tools are important to select, multiply, improve and analyze medicinal plants for production of such products. The utilization of medicinal plant cells for the production of natural or recombinant compounds of commercial interest has gained increasing attention over the past decades. Plant tissue culture systems are possible source of valuable medicinal compounds, fragrances and colorants, which cannot be produced by microbial cells or chemical synthesis. In vitro production of bioactive natural products in plant cell suspension culture has been reported from various medicinal plants and bioreactors are the key step towards commercial production. Genetic transformation is a powerful tool for enhancing the productivity of novel products; especially by Agrobacterium tumefacians. Combinatorial biosynthesis is another approach in the generation of novel natural products and for the production of rare and expensive natural products. Recent advances in the molecular biology, enzymology and bioreactor technology of plant cell culture suggest that these systems may become a viable source of important secondary metabolites. Genetic fingerprinting could be a powerful tool in the field of medicinal plants to be used for correct germplasm identification. In addition, when linked to emerging tools such as metabolomics and proteomics, providing fingerprints of the plant's metabolites or protein composition, it gives data on phenotypic variation, caused by growth conditions or environmental factors, and also yield data on the genes involved in the biosynthesis. DNA profiling techniques like DNA microarrays serve as suitable high throughput tools for the simultaneous analysis of multiple genes and analysis of gene expression that becomes necessary for providing clues about regulatory mechanisms, biochemical pathways and broader cellular functions. New and powerful tools in functional genomics can be used in combination with metabolomics to elucidate biosynthetic pathways of natural products.

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Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture

Cited by 9 publications

References 13 publications

Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]

Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

Bioactive Natural Products from Plants and Biotechnological Approaches for their Production

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