OsRAD17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly

Hu, Qing; Zhang, Chao; Xue, Zhihui; Ma, Lijun; Liu, Wei; Shen, Yi; Ma, Bo; Cheng, Zhukuan

doi:10.3389/fpls.2018.01236

Cited by 10 publications

(13 citation statements)

References 48 publications

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“…Chromosome fragmentation is a characteristic phenomenon observed in mutants defective in DSB repair machinery. Our results showed that the Zmrad51c mutant phenotype is similar to that of the Osrad51c and Atrad51c mutants, as well as other related mutants such as Osxrcc3 [44], Osrad1 [81], Osrad17 [82], Atrad50 [83], and Atmre11 [84]. However, the severity of the chromosome segregation seems to be much less in Zmrad51c compared to the Osrad51c and Atrad51c mutants.…”

Section: Discussionsupporting

confidence: 63%

ZmRAD51C Is Essential for Double-Strand Break Repair and Homologous Recombination in Maize Meiosis

Jing

Zhang

Wang

et al. 2019

IJMS

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Radiation sensitive 51 (RAD51) recombinases play crucial roles in meiotic double-strand break (DSB) repair mediated by homologous recombination (HR) to ensure the correct segregation of homologous chromosomes. In this study, we identified the meiotic functions of ZmRAD51C, the maize homolog of Arabidopsis and rice RAD51C. The Zmrad51c mutants exhibited regular vegetative growth but complete sterility for both male and female inflorescence. However, the mutants showed hypersensitivity to DNA damage by mitomycin C. Cytological analysis indicated that homologous chromosome pairing and synapsis were rigorously inhibited, and meiotic chromosomes were often entangled from diplotene to metaphase I, leading to chromosome fragmentation at anaphase I. Immunofluorescence analysis showed that although the signals of the axial element absence of first division (AFD1) and asynaptic1 (ASY1) were normal, the assembly of the central element zipper1 (ZYP1) was severely disrupted. The DSB formation was normal in Zmrad51c meiocytes, symbolized by the regular occurrence of γH2AX signals. However, RAD51 and disrupted meiotic cDNA 1 (DMC1) signals were never detected at the early stage of prophase I in the mutant. Taken together, our results indicate that ZmRAD51C functions crucially for both meiotic DSB repair and homologous recombination in maize.

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Section: Discussionsupporting

confidence: 63%

ZmRAD51C Is Essential for Double-Strand Break Repair and Homologous Recombination in Maize Meiosis

Jing

Zhang

Wang

et al. 2019

IJMS

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“…In contrast, the loss-offunction of RAD17 caused similar but much less severe effects on meiotic chromosome behaviors in maize, exemplified by subtle chromosome entanglement and fragmentation. Particularly, the unidirectional abnormality in male meiosis from the dysfunction of the maize RAD17 seems strikingly different from rice, where both male and female were aborted (Hu et al, 2018). These findings highlight that although the participation of RAD17 homologs in DSB repair is widely conserved, the precise effects of RAD17 on meiosis seem divergent among different organisms.…”

Section: Discussionmentioning

confidence: 91%

“…In the Zmrad17 mutant, the disturbed loading of ZYP1 protein supports the functional conservation of RAD17 in the SC installation between yeast and plant. In contrast, the SC formation seemed roughly normal in the Osrad17 mutant, and the incomplete SC formation only occurred after combining Osrad17 with mutation in ZMM proteins, such as ZIP4 or MSH5, implying that OsRAD17 has to work cooperatively with ZMM proteins to promote homologous pairing and synapsis in rice (Hu et al, 2018). In this context, the redundancy between RAD17 and ZMM proteins in regulating the SC installation may not be critical in maize.…”

Section: Discussionmentioning

confidence: 95%

“…In the model plant Arabidopsis, the defective RAD17 was considered to have no strong effects on meiosis due to the normal fertility of both male and female, whereas the mutant displayed hypersensitive to DNA-damaging agents with the frequent presence of intrachromosomal HR during mitosis (Heitzeberg et al, 2004). In rice, the disruption of RAD17 resulted in massive abnormal associations between non-homologous chromosomes, leading to enormous chromosome aggregations and fragments during male meiosis (Hu et al, 2018). In contrast, the loss-offunction of RAD17 caused similar but much less severe effects on meiotic chromosome behaviors in maize, exemplified by subtle chromosome entanglement and fragmentation.…”

Section: Discussionmentioning

confidence: 99%

“…In plant, the mutation in AtRAD17 led to hypersensitivity to the DNA-damaging agent treatment, whereas mutant plants were fully fertile, suggesting that the RAD17 may not play an important role in Arabidopsis meiosis (Heitzeberg et al, 2004). In contrast, the disruption in OsRAD17 resulted in aberrant associations between non-homologous chromosomes, leading to massive chromosome entanglements and fragmentations, indicating that the OsRAD17 is essential for meiotic DSB repair in rice (Hu et al, 2018). The marked dissimilarity of meiotic outcomes caused by the defective RAD17 raises an intriguing question that whether the role of RAD17 in meiosis is conserved across plant kingdom.…”

Section: Introductionmentioning

confidence: 99%

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ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis

et al. 2021

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RAD17, a replication factor C (RFC)-like DNA damage sensor protein, is involved in DNA checkpoint control and required for both meiosis and mitosis in yeast and mammals. In plant, the meiotic function of RAD17 was only reported in rice so far. Here, we identified and characterized the RAD17 homolog in maize. The Zmrad17 mutants exhibited normal vegetative growth but male was partially sterile. In Zmrad17 pollen mother cells, non-homologous chromosome entanglement and chromosome fragmentation were frequently observed. Immunofluorescence analysis manifested that DSB formation occurred as normal and the loading pattern of RAD51 signals was similar to wild-type at the early stage of prophase I in the mutants. The localization of the axial element ASY1 was normal, while the assembly of the central element ZYP1 was severely disrupted in Zmrad17 meiocytes. Surprisingly, no obvious defect in female sterility was observed in Zmrad17 mutants. Taken together, our results suggest that ZmRAD17 is involved in DSB repair likely by promoting synaptonemal complex assembly in maize male meiosis. These phenomena highlight a high extent of divergence from its counterpart in rice, indicating that the RAD17 dysfunction can result in a drastic dissimilarity in meiotic outcome in different plant species.

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Yield component QTLs identified by genome‐wide association mapping validated in a diverse tropical japonica × tropical japonica rice biparental mapping population

Eizenga

Rice²,

Huggins

et al. 2023

Crop Science

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The Rice Diversity Panel 1 (RDP1) is a resource for genome‐wide association (GWA) studies to explore the five major rice (Oryza sativa L.) subpopulations indica, aus, aromatic, temperate japonica, and tropical japonica (TRJ). Previously collected phenotype data for yield‐related traits in RDP1 were reanalyzed using a recently released 4.8 million single nucleotide polymorphism (SNP) dataset to enhance GWA mapping precision. GWA mapping revealed numerous GWA quantitative trait loci (QTLs) that were specific to one or more subpopulations. Most southern US cultivars are classified tropical japonica (TRJ) or TRJ admixtures, thus TRJ was the focus for validating Rice Diversity Panel 1 GWA QTLs. Two diverse TRJ accessions, L‐202 originating from California, USA, and Trembese from Indonesia, were selected for developing a recombinant inbred line (RIL) population. This RIL population was phenotyped for 20 yield component traits, 16 of which were measured in RDP1, including six agronomic, six panicle architecture, and four seed traits. Genotyping with 1439 polymorphic markers and QTL mapping revealed 43 RIL QTLs. Examination of the GWA QTLs identified 163 GWA QTLs for the same or related traits co‐located with 37 RIL QTLs. A significant overlap was on chromosome 7 (21.8–25.4; 26.8–29.6 Mb) where the well‐known yield related genes, GRAIN WEIGHT7 (GW7) and FRIZZY PANICLE (FZP), respectively, are located and specific to TRJ. In this region, 10 RIL QTLs were co‐located with 36 GWA QTLs for panicle architecture or seed traits, especially secondary branching to increase the number of seeds. RIL QTLs co‐located with GWA QTLs are targets for developing SNP markers that can be utilized in cultivar development.

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OsRAD17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly

Cited by 10 publications

References 48 publications

ZmRAD51C Is Essential for Double-Strand Break Repair and Homologous Recombination in Maize Meiosis

ZmRAD51C Is Essential for Double-Strand Break Repair and Homologous Recombination in Maize Meiosis

ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis

Yield component QTLs identified by genome‐wide association mapping validated in a diverse tropical japonica × tropical japonica rice biparental mapping population

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