OsSCE1 Encoding SUMO E2-Conjugating Enzyme Involves in Drought Stress Response of Oryza sativa

Nurdiani, Dini; Widyajayantie, Dwi; Nugroho, Satya

doi:10.1016/j.rsci.2017.11.002

Cited by 22 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, drought and combined stress showed a higher buildup of proline, which is in line with the findings of (Cvikrová et al 2013) who demonstrated that the higher levels of proline are involved in plant protection against combined drought and heat stress. Several recent studies also support our findings that proline accumulation occurs in plants exposed to stress conditions (Claussen 2005;Kaur and Asthir 2015;Moreno-Galván et al 2020) because of its property to stabilize subcellular structures, scavenging free radicals and buffer cellular redox potential (Dar et al 2016;Hazman et al 2015;Nurdiani et al 2018). Under different stress conditions, plants maintain their physiological balance through higher RWC values particularly under higher rates of transpiration normally.…”

Section: Discussionsupporting

confidence: 87%

Impact of drought and heat stress individually and in combination on physio-biochemical parameters, antioxidant responses, and gene expression in Solanum lycopersicum

Raja¹,

Qadir²,

et al. 2020

View full text Add to dashboard Cite

The present study was carried out to investigate the effect of individual drought, heat, and combined drought and heat stress on tomato plants. Combined stress resulted in the higher accumulation of Proline (101.9%), MDA (38.55%), H 2 O 2 (101.19%), and lower levels of RWC (53.84%). Individual drought and heat stress decreased photosynthetic pigments like total chlorophyll content by (45.45%) and (25.35%), respectively, higher rates of pigment reduction (79.42%) were observed under combined drought and heat stress. Combined stress decreased PSII efficiency (Fv/Fm), quantum yield (ΦPSII), and photochemical efficiency (qp) and increased non-photochemical quenching (NPQ) levels. Moreover, the gas exchange parameters E, A, and Pn decreased by 5.36%, 36.45%, and 51.00%, respectively, in comparison to control plants. Antioxidant enzymes, SOD, APX, CAT, and GR showed a two-to threefold increase under combined drought and heat stress; however, the non-enzymatic antioxidants AsA and GSH displayed one-twofold increase under combined stress. Moreover, 2-to 2.5fold decrease was observed in MDHAR and DHAR enzyme transcripts under combined stress conditions. The transcripts corresponding to AsA-GSH pathway enzymes SOD, APX, GR, DHAR, and MDHAR were up-regulated by 8-to 12-fold under combined drought and heat. Furthermore, DREB and LEA transcripts were up-regulated under drought and combined stress and down-regulated under drought stress. In the same manner, HSP70 and HSP90 transcripts were up-regulated under heat and combined stress; however, the transcription levels got down-regulated under drought stress. Additionally, rbcL and RCA transcripts were down-regulated especially under combined stress in comparison to individual drought and heat conditions. PSIP680 relative expression levels were up-regulated under drought stress; however, the transcripts were downregulated under heat and combined stress. Taken together, the results suggest that the combined stress has a predominant effect over individual stress.

show abstract

Section: Discussionsupporting

confidence: 87%

Impact of drought and heat stress individually and in combination on physio-biochemical parameters, antioxidant responses, and gene expression in Solanum lycopersicum

Raja¹,

Qadir²,

et al. 2020

View full text Add to dashboard Cite

show abstract

“…SAE2 is an essential large sub-unit for the SUMO-activating enzyme (SAE) in SUMOlaytion [43]. SUMOlaytion is another important form of post-translational modification similar to UPP, which also plays crucial roles in regulating what when responding to abiotic stress responses, particularly in plant drought stress [44–46]. Some studies suggest that SAE may act as a limiting regulatory step during SUMO conjugation [43, 47].…”

Section: Discussionmentioning

confidence: 99%

PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles

Liu

Zhang

et al. 2019

BMC Plant Biol

View full text Add to dashboard Cite

BackgroundIntermittent dehydration caused by tidal changes is one of the most important abiotic factors that intertidal seaweeds must cope with in order to retain normal growth and reproduction. However, the underlying molecular mechanisms for the adaptation of red seaweeds to repeated dehydration-rehydration cycles remain poorly understood.ResultsWe chose the red seaweed Gloiopeltis furcata as a model and simulated natural tidal changes with two consecutive dehydration-rehydration cycles occurring over 24 h in order to gain insight into key molecular pathways and regulation of genes which are associated with dehydration tolerance. Transcription sequencing assembled 32,681 uni-genes (GC content = 55.32%), of which 12,813 were annotated. Weighted gene co-expression network analysis (WGCNA) divided all transcripts into 20 modules, with Coral2 identified as the key module anchoring dehydration-induced genes. Pathways enriched analysis indicated that the ubiquitin-mediated proteolysis pathway (UPP) and phosphatidylinositol (PI) signaling system were crucial for a successful response in G. furcata. Network-establishing and quantitative reverse transcription PCR (qRT-PCR) suggested that genes encoding ubiquitin-protein ligase E3 (E3–1), SUMO-activating enzyme sub-unit 2 (SAE2), calmodulin (CaM) and inositol-1,3,4-trisphosphate 5/6-kinase (ITPK) were the hub genes which responded positively to two successive dehydration treatments. Network-based interactions with hub genes indicated that transcription factor (e.g. TFIID), RNA modification (e.g. DEAH) and osmotic adjustment (e.g. MIP, ABC1, Bam1) were related to these two pathways.ConclusionsRNA sequencing-based evidence from G. furcata enriched the informational database for intertidal red seaweeds which face periodic dehydration stress during the low tide period. This provided insights into an increased understanding of how ubiquitin-mediated proteolysis and the phosphatidylinositol signaling system help seaweeds responding to dehydration-rehydration cycles.

show abstract

“…Surprisingly, a recent work has shown that overexpression of the SCE1 rice homologue (OsSCE1) confers osmotic sensitivity to transgenic rice plants in PEG6000 treatment assays, while OsSCE1 downregulation confers osmotic resistance (Nurdiani et al, 2018). Osmotic tolerance in plants with OsSCE1 downregulation is also correlated with an increase in proline content, suggesting that indeed OsSCE1 maybe be regulating proline production under stress conditions (Nurdiani et al, 2018).…”

Section: Activation Of Sumoylation and Drought Stressmentioning

confidence: 99%

“…For instance, the Arabidopsis SUMO conjugating enzyme SCE1 levels are increased in siz1 mutants (Castaño-Miquel et al, 2017;Saracco et al, 2007) and attempts to overexpress SCE1 have resulted in co-suppression (Lois et al, 2003) or silencing (Tomanov et al, 2013). If these SCE1 properties are conserved across evolution, drought tolerance results generated by SCE1 manipulation, as in (Nurdiani et al, 2018), should be taken cautiously since the SUMO conjugation capacity of the transgenic plants was not analyzed. Only a deep molecular characterization of plants studied will provide robust conclusions about the role of SUMOylation in drought tolerance.…”

Section: Activation Of Sumoylation and Drought Stressmentioning

confidence: 99%

Sumoylation in plants: mechanistic insights and its role in drought stress

Benlloch

Lois

2018

Journal of Experimental Botany

View full text Add to dashboard Cite

Post-translational modification by SUMO is an essential process that has a major role in the regulation of plant development and stress responses. Such diverse biological functions are accompanied by functional diversification among the SUMO conjugation machinery components and regulatory mechanisms that has just started to be identified in plants. In this review, we focus on the current knowledge of the SUMO conjugation system in plants in terms of components, substrate specificity, cognate interactions, enzyme activity, and subcellular localization. In addition, we analyze existing data on the role of SUMOylation in plant drought tolerance in model plants and crop species, paying attention to the genetic approaches used to stimulate or inhibit endogenous SUMO conjugation. The role in drought tolerance of potential SUMO targets identified in proteomic analyses is also discussed. Overall, the complexity of SUMOylation and the multiple genetic and environmental factors that are integrated to confer drought tolerance highlight the need for significant efforts to understand the interplay between SUMO and drought.

show abstract

OsSCE1 Encoding SUMO E2-Conjugating Enzyme Involves in Drought Stress Response of Oryza sativa

Cited by 22 publications

References 28 publications

Impact of drought and heat stress individually and in combination on physio-biochemical parameters, antioxidant responses, and gene expression in Solanum lycopersicum

Impact of drought and heat stress individually and in combination on physio-biochemical parameters, antioxidant responses, and gene expression in Solanum lycopersicum

PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles

Sumoylation in plants: mechanistic insights and its role in drought stress

Contact Info

Product

Resources

About