Osteo/odontogenic Differentiation of Human Mesenchymal Stem Cells with Platelet-rich Plasma and Mineral Trioxide Aggregate

Vanka, Amit; Vishwakarma, Sandeep Kumar; Bhat, Manohar; Vanka, Shanthi; Wali, Othman; Khan, Aleem Ahmed

doi:10.5005/jp-journals-10024-2677

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…Positive staining of the cells with alizarin red indicates the presence of calcium deposition, and the quantity of mineralization was much higher in the PRP and MTA combined group. These results were similar to findings from a previous study 8 .…”

Section: Discussionsupporting

confidence: 93%

“…Mineral Trioxide Aggregate (MTA) is a biocompatible material used in several clinical applications in dentistry, including sealing the orifice during revacularization 7 . A study has demonstrated that the combination of PRP and MTA is capable of enhancing the differentiation of cells towards an osteo/ odontogenic lineage 8 . Nevertheless, the phenotype of the cells and mineralized deposits formed during the differentiation process has not been analyzed.…”

Section: Biochemical Evaluation Of Alkaline Phosphatase (Alp) Activitymentioning

confidence: 99%

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Gene Expression during Osteo / Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate

Vanka¹,

Vanka²,

Vishwakarma³

et al. 2019

Asian Journal of Pharmaceutical Research and Health Care

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Platelet Rich Plasma (PRP) has the potential to regenerate pulp in immature pulpless teeth. Mineral trioxide is commonly used to seal the PRP into the pulp canal space. We investigated the effect of PRP and MTA individually and combined on osteo/odontogenic differentiation potential and the phenotype of tissue formed. MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5% PRP and MTA with 10% PRP. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining. Relative expression of Alkaline Phosphatase (ALP) activity, type 1 collagen (COL1A1), Dental Sialo-Phospho Protein (DSPP), Dentin Matrix Protein (DMP-1), Bone Gamma-carboxyglutamate Protein (BGLAP), Runt-related transcription factor 2 (Runx2), Osterix (Osx) and TGF-β1 was identified by RT-q PCR. 10% PRP with MTA displayed significantly higher calcium deposition during differentiation and high ALP levels. Significantly enhanced levels of DSPP, DMP1, COL1A1, BGLAP, Runx2, and Osx and TGF-β1 transcripts were observed. Within limitations of the in vitro environment, results imply enhanced osteodentin formation, on combining PRP with MTA.

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Section: Discussionsupporting

confidence: 93%

Section: Biochemical Evaluation Of Alkaline Phosphatase (Alp) Activitymentioning

confidence: 99%

Gene Expression during Osteo / Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate

Vanka¹,

Vanka²,

Vishwakarma³

et al. 2019

Asian Journal of Pharmaceutical Research and Health Care

Self Cite

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“…Moreover, a recent in vitro study established that MTA and PRP can initiate the pulp tissue’s regeneration activity via IPMSCs [ 34 ]. Our results showed a similar tendency to those of previous studies on direct pulp capping, but the combination of MTA and PRP seemed to promote the differentiation of IPMSCs into odontoblasts and dentin bridge hard-tissue formation in the early stage of treatment [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar

et al. 2021

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Mineral trioxide aggregate (MTA) is an alternative endodontic material that predicts conductive or inductive calcified tissue formation from immature pulp mesenchymal stem cells (IPMSCs). The purpose of this study was to investigate whether MTA could promote reparative odontoblast differentiation via IPMSCs in the early phase of regeneration and compare with calcium hydroxide (CH). Direct pulp capping using calcium hydroxide (CH), MTA, and MTA with platelet-rich plasma (MTA + PRP) was performed on maxillary first molars of 8-week-old male Wistar rats (n = 36). After 3, 7, or 14 days, the teeth were analyzed for mineral density (MD) and volume of MD (VMD) via micro-focusing computed tomography (µCT), nestin, dentin matrix acidic phosphoprotein 1 (DMP1) immunohistochemistry, and real-time PCR for DMP1 mRNA expression. MTA stimulated the early phase differentiation of the IPMSCs into odontoblasts, with positive results for nestin and DMP1 compared with CH. Moreover, MTA + PRP stimulated calcified granule and dentin bridge formation through calcium mineral deposition, following the induction of DMP1 mRNA expression in IPMSCs. Our results suggested that the combination of MTA and PRP is an effective and clinically applicable method for activating endogenous dental pulp stem cells into odontoblasts in the early stages of pulp regeneration.

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“…A total of 46 hCSCs were used in our included studies: 28 commercially available hCSCs along with 18 different modifications of hCSCs. PRMTA (n = 40) and BD (n = 34) were the most frequently used cements amongst all of the studies [ 37 , 38 , 39 , 41 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , …”

Section: Resultsmentioning

confidence: 99%

Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review

Yousefi-Koma,

Assadian,

Mohaghegh

et al. 2023

JFB

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Background: Regenerative dentistry is the operation of restoring dental, oral and maxillofacial tissues. Currently, there are no guidelines for the ideal cement/material in regenerative endodontic treatments (RET). Hydraulic calcium silicate-based cements (hCSCs) are currently the material of choice for RET. Objectives: This systematic review was conducted to gather all of the different direct and indirect approaches of using hCSCs in RET in vitro and in vivo, and to ascertain if there are any superiorities to indirect approaches. Methods and Materials: This systematic review was conducted according to the 2020 PRISMA guidelines. The study question according to the PICO format was as follows: Comparison of the biological behavior (O) of stem cells (P) exposed to hCSCs through direct and indirect methods (I) with untreated stem cells (C). An electronic search was executed in Scopus, Google Scholar, and PubMed. Results: A total of 78 studies were included. Studies were published between 2010 and 2022. Twenty-eight commercially available and eighteen modified hCSCs were used. Seven exposure methods (four direct and three indirect contacts) were assessed. ProRoot MTA and Biodentine were the most used hCSCs and had the most desirable results. hCSCs were either freshly mixed or set before application. Most studies allowed hCSCs to set in incubation for 24 h before application, which resulted in the most desirable biological outcomes. Freshly mixed hCSCs had the worst outcomes. Indirect methods had significantly better viability/proliferation and odonto-/osteogenesis outcomes. Conclusion: Biodentine and ProRoot MTA used in indirect exposure methods result in desirable biological outcomes.

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Osteo/odontogenic Differentiation of Human Mesenchymal Stem Cells with Platelet-rich Plasma and Mineral Trioxide Aggregate

Cited by 5 publications

References 32 publications

Gene Expression during Osteo / Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate

Gene Expression during Osteo / Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate

Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar

Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review

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