Osteoblast activity on carbonated hydroxyapatite

Rupani, Asha; Hidalgo‐Bastida, Araida; Rutten, Frank J. M.; Dent, Andrew C E; Turner, I G; Cartmell, Sarah H.

doi:10.1002/jbm.a.34037

Cited by 58 publications

(45 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present work, the cytotoxicity of gentamicin-loaded MCHMs was assessed by the MTT assay using the hBMSCs as a cell model. The HAPs are used as a control group because hydroxyapatite has been confirmed to have excellent biocompatibility and bioactivity [49,50]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Bactericidal property and biocompatibility of gentamicin-loaded mesoporous carbonated hydroxyapatite microspheres

Guo

Long

Wei

et al. 2013

Materials Science and Engineering: C

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Bactericidal property and biocompatibility of gentamicin-loaded mesoporous carbonated hydroxyapatite microspheres

Guo

Long

Wei

et al. 2013

Materials Science and Engineering: C

View full text Add to dashboard Cite

“…Predominantly, the carbonate ions are present as B-type carbonates in natural bone minerals [3]. Synthetic CHAs have been widely used in a variety of biomedical applications including osteoconductive coatings [4][5][6], bone-substitute biomaterials [7], and vehicles for drug delivery [ 8]. Recently, hydoxyapatite nanorods have been prepared by an ethanol-induced method [9], liquid crystals [10], sonochemical synthesis [11], sol-gel method [12], and hydrothermal reaction [13,14].…”

mentioning

confidence: 99%

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Xue¹,

Chen²,

Huang³

et al. 2016

Nano Online

View full text Add to dashboard Cite

(http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.Keywords: Hydroxyapatite; Carbonated hydroxyapatite; Nanorods; Cell viability; Alkaline phosphatase activity; MG-63 cell BackgroundHydroxyapatite (HA) is the main inorganic component of human bone mineral, and the content of carbonate in human bone mineral is about 5-8 wt % [1, 2]. Carbonate ions in carbonated hydroxyapatites (CHA) substitute both the phosphate and hydroxyl sites of the HA structure and each is called A-type CHA and B-type CHA, respectively. Predominantly, the carbonate ions are present as B-type carbonates in natural bone minerals [3]. Synthetic CHAs have been widely used in a variety of biomedical applications including osteoconductive coatings [4][5][6], bone-substitute biomaterials [7], and vehicles for drug delivery [ 8]. Recently, hydoxyapatite nanorods have been prepared by an ethanol-induced method [9], liquid crystals [10], sonochemical synthesis [11], sol-gel method [12], and hydrothermal reaction [13,14]. However, few methods have been reported for the preparation of carbonated hydroxyapatite nanorods with different carbonate contents. Since carbonate ion substitution in the apaptite lattice plays a major role in the biochemistry and physical properties of biological apatites, it is important to develop convenient ways for the synthesis of CHA nanorods with different carbonate contents and understand how various carbonate contents affect the crystal structure and biocompatibility of CHA nanorods. Table 1 Synthesizing materials for preparing HA and CHA nanorods SamplesCaThe hydrothermal method is a typical process which has been widely used in synthesis of inorganic materials for its good repeatability and crystallinity control [15][16][17]. In this study, we developed a hydrothermal process to synthesize carbonated hydroxyapatite nanorods with different carbonate contents, using ethylene diamine tetraacetic aci...

show abstract

“…14 The bands at 1452 and 1419 cm 21 correspond to carbonate group produced by CO 2 in the air. 15 The bands at 1655 and 1560 cm 21 correspond to amide I and amide II of the collagen, respectively. Moreover, the absorption band at about 1240 cm 21 for amide III is almost disappeared, since the carbonyl groups on the surface of collagen molecules are the nucleation sites of the mineral.…”

Section: Statisticsmentioning

confidence: 99%

Releasing profile of antibiotics from a novel mineralized collagen putty with quantifiable drug loading

Xie¹,

Wang²,

Wang³

et al. 2015

Materials Technology

View full text Add to dashboard Cite

2015) Releasing profile of antibiotics from a novel mineralized collagen putty with quantifiable drug loading, Materials Technology, 30:sup8, B216-B222,This study aims to compare the release profile of antibiotics when loaded onto a new mineralized collagen (MC) putty with conventional polymethyl methacrylate (PMMA) cement as well as their feasibility for clinical application for orthopaedic wounds. Two types of antibiotic-loaded implants were fabricated by physically mixing Imipenem or Vancomycin with either PMMA or MC in a quantifiable manner. Subsequently, the release profiles of antibiotics and their inhibitory effects on bacterial growth were evaluated both in vitro and in vivo. In comparison to PMMA, the antibioticloaded MC putty was easier to make with high flexibility of antibiotic selection based on drug sensitivity test, and surgeon friendly moldability. The inhibitory effects of bacterial growth lasted 24 h post-intramuscular implantation. The Imipenem-loaded MC putty was confirmed to be superior as a local drug delivery system (LDDS) over PMMA cement, the only type of antibiotics carrier orthopaedic clinics currently use.

show abstract

Osteoblast activity on carbonated hydroxyapatite

Cited by 58 publications

References 44 publications

Bactericidal property and biocompatibility of gentamicin-loaded mesoporous carbonated hydroxyapatite microspheres

Bactericidal property and biocompatibility of gentamicin-loaded mesoporous carbonated hydroxyapatite microspheres

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Releasing profile of antibiotics from a novel mineralized collagen putty with quantifiable drug loading

Contact Info

Product

Resources

About