Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography

Lenhert, Steven; Meier, M. R.; Meyer, Ulrich; Chi, Lifeng; Wiesmann, Hans Peter

doi:10.1016/j.biomaterials.2004.02.068

Cited by 173 publications

(148 citation statements)

References 23 publications

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…In addition, pattern depth greatly influenced osteoblast morphology, independent of spacing. In agreement with previous studies, a threshold for the response to pattern depth was found at ~34 nm (Lenhert et al, 2005;Loesberg et al, 2008). The inability to respond to patterns with a depth less than 34 nm may be hypothesized to be a result of clogging of the surface features in a protein rich environment.…”

Section: Discussionsupporting

confidence: 91%

The influence of nanoscale topographical cues on initial osteoblast morphology and migration

Lamers

Horssen²,

Riet³

et al. 2010

eCM

136

124

View full text Add to dashboard Cite

The natural environment of a living cell is not only organized on a micrometer, but also on a nanometer scale. Mimicking such a nanoscale topography in implantable biomaterials is critical to guide cellular behavior. Also, a correct positioning of cells on biomaterials is supposed to be very important for promoting wound healing and tissue regeneration. The exact mechanism by which nanotextures can control cellular behavior are thus far not well understood and it is thus far unknown how cells recognize and respond to certain surface patterns, whereas a directed response appears to be absent on other pattern types. Focal adhesions (FAs) are known to be involved in the process of specific pattern recognition and subsequent response by cells. In this study, we used a high throughput screening "Biochip" containing 40 different nanopatterns to evaluate the influence of several nanotopographical cues like depth, width, (an)isotropy and spacing (ridge-groove ratio) on osteoblast behavior. Microscopical analysis and time lapse imaging revealed that an isotropic topography did not alter cell morphology, but it highly induced cell motility. Cells cultured on anisotropic topographies on the other hand, were highly elongated and aligned. Time-lapse imaging revealed that cell motility is highly dependent on the ridgegroove ratio of anisotropic patterns. The highest motility was observed on grooves with a ratio of 1:3, whereas the lowest motility was observed on ratios of 1:1 and 3:1. FA measurements demonstrated that FA-length decreased with increasing motility. From the study it can be concluded that osteoblast behavior is tightly controlled by nanometer surface features.

show abstract

Section: Discussionsupporting

confidence: 91%

The influence of nanoscale topographical cues on initial osteoblast morphology and migration

Lamers

Horssen²,

Riet³

et al. 2010

eCM

136

124

View full text Add to dashboard Cite

show abstract

“…A wide range of cell types, such as fibroblasts (Dalby et al, 2003b), osteoblasts (Lenhert et al, 2005) and mesenchymal stem cells (Biggs et al, 2008), are influenced by nanoscale grooves with dimensions that mimic those in vivo. Cellular morphology depends on cell type and on groove depth and width.…”

Section: Nanosurfacesmentioning

confidence: 99%

Novel Promises of Nanotechnology for Tissue Regeneration

Sadik¹

2012

Tissue Regeneration - From Basic Biology to Clinical Application

View full text Add to dashboard Cite

“…The surface topography in micrometer-and nanometer-scales has been shown to affect various cell functions, such as extracellular calcium deposition, ALP activity, and production of extracellular osteocalsin. 9)12) The presence of nanotubes on a Ti surface activated the extracellular calcium deposition of CRL-11372 human osteoblasts more than it did on a flat surface. 13) Choi et al reported that the number and cell size of human foreskin fibroblasts decreased with increasing the domain gaps from 50 to 600 nm.…”

Section: )mentioning

confidence: 99%

Control of cellular activity of osteoblastic cells with microtopography of biphasic calcium phosphate scaffolds

Okada¹,

Oaki²,

Komotori³

et al. 2011

J. Ceram. Soc. Japan

View full text Add to dashboard Cite

We studied the activity of osteoblastic cells on biphasic calcium phosphate (BCP) containing ca. 50 wt % hydroxyapatite and ca. 50 wt % ¢-tricalcium phosphate. The influence of the microtopography on the cellular activity was investigated by using three kinds of BCP scaffolds, which were composed of densely packed domains of 35 µm in size (dense-BCP), a porous framework of ³1 µm in width (micro-BCP), and aggregated particles of ³100 nm in diameter (nano-BCP). Basically, stable adhesion of the cells was achieved with defined focal adhesions on the BCP scaffolds regardless of their topography. The alkaline phosphatase (ALP) activity in the initial stage of the adhesion was more enhanced on micro-and nano-BCPs than it was on dense-BCPs due to the presence of a large number of calcium ions eluted from small grains in the micro-and nano-BCPs. However, the proliferation and the ALP activity were suppressed in the long-term culture on nano-BCP because of the limitation of the domain area for the formation of focal adhesions. Therefore, the cellular activity was successfully controlled with the surface microtopography of the scaffolds. The microstructured surface providing a sufficient domain area and essential ions would be suitable as a scaffold for osteoblastic cells.

show abstract

Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography

Cited by 173 publications

References 23 publications

The influence of nanoscale topographical cues on initial osteoblast morphology and migration

The influence of nanoscale topographical cues on initial osteoblast morphology and migration

Novel Promises of Nanotechnology for Tissue Regeneration

Control of cellular activity of osteoblastic cells with microtopography of biphasic calcium phosphate scaffolds

Contact Info

Product

Resources

About