Osteoblast growth, after cleaning of biofilm‐covered titanium discs with air‐polishing and cold plasma

Matthes, Rutger; Duske, Kathrin; Kebede, Tewodros Getachew; Pink, Christiane; Schlüter, Rabea; Woedtke, Thomas von; Weltmann, Klaus‐Dieter; Kocher, Thomas; Jablonowski, Lukasz

doi:10.1111/jcpe.12720

Cited by 37 publications

(69 citation statements)

References 44 publications

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“…After reading the full‐text articles, 10 articles were excluded: three articles due to using the AA in combination with other decontamination methods, one study was not about titanium surface, two articles did not use AA for decontamination, one study was not in English and not published in a peer‐review journal, one study used the AA powder in an antimicrobial solution but did not use the AA device, one study had an in vivo design with decontamination procedures taking place in the beagle dogs, and the full text for one study was not retrieved . The 48 included articles were ultimately processed for data extraction . The summary of the search strategy is depicted in Figure .…”

Section: Resultsmentioning

confidence: 99%

“…The characteristics of the 48 selected studies have been reported in chronological order in Table . It is noteworthy to mention that out of 48 included studies, only 11 studies employed devices to standardize the decontamination procedure and avoid the clinician erroneous, and only one study used a phantom head to simulate clinical conditions …”

Section: Resultsmentioning

confidence: 99%

“…Fibroblast proliferation rate was found to be slightly higher on the SLA surface treated with AA compared with Er: YAG laser and profoundly higher on the nonmodified and plasma‐sprayed surfaces compared with plastic curettes . Osteoblast‐like cells viability was improved by AA compared with the plastic curette, titanium brush, implantoplasty, and cold atmospheric plasma (CAP) . AA with glycine was weaker than CAP and high‐density chlorohexidine in preventing regrowth of oral biofilm, but AA with erythritol was superior to CAP …”

Section: Resultsmentioning

confidence: 99%

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Effects of air abrasive decontamination on titanium surfaces: A systematic review of in vitro studies

Moharrami¹,

Perrotti

Iaculli

et al. 2019

Clin Implant Dent Rel Res

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Background Air abrasion (AA) is one of the decontamination methods that have demonstrated promising results in treating peri‐implant diseases. Purpose This systematic review aimed at evaluating the in vitro effect of AA on surface change, cleaning efficacy, and biocompatibility of titanium surfaces and at comparing it with other decontamination methods. Materials and Methods A comprehensive search was conducted up to April 2018 using PubMed, Scopus, and Google Scholar databases to identify studies on the decontamination effect of AA. All types of titanium surfaces, abrasive powders, contaminated surfaces, and measuring methods were included. Results Overall, 1502 articles were identified. After screening the titles and abstracts, and carefully reading the full‐texts, 48 articles published between 1989 and 2018 were selected. AA was considered almost safe, particularly for the nonmodified surfaces. Nevertheless, harder powders such as sodium bicarbonate tended to damage the surface more than glycine. AA resulted in surface change similar to plastic curettes and Er: YAG lasers. Regarding the cleaning efficacy, there was no significant difference between glycine and sodium bicarbonate, but different mixtures of calcium phosphate, hydroxyapatite, and erythritol were superior to glycine. AA was superior or equal to all other decontamination methods in cleaning. Regarding biocompatibility, AA was more successful in preserving biocompatibility for noncontaminated surfaces compared with contaminated surfaces and when used with erythritol and osteoinductive powders. Conclusions AA can efficiently remove contamination without serious damage to the surface. The main drawback of the AA method seems to be its limitation in restoring the biocompatibility of the surface.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of air abrasive decontamination on titanium surfaces: A systematic review of in vitro studies

Moharrami¹,

Perrotti

Iaculli

et al. 2019

Clin Implant Dent Rel Res

View full text Add to dashboard Cite

show abstract

“…However, it is important to note that killing the biofilm is not enough for its removal, considering there are other biofilm products that play a role in peri‐implantitis, including cytokines . It was demonstrated that removal of dead microbial residues was equally necessary to promote cell adhesion . The visual analysis of the confocal images of the present study showed fewer microorganisms in plasma‐treated samples than in negative control groups.…”

Section: Discussionmentioning

confidence: 54%

“…Although these findings indicate a possible mechanical removal of dead biofilm, the LTP potential to remove dead bacterium is still controversial. A recent study used 7‐day‐old biofilms to investigate the cleaning efficacy of air polishing with or without additional cold plasma treatment and concluded that concomitant use of air polishing and plasma treatment did not enhance osteoblast spreading …”

Section: Discussionmentioning

confidence: 99%

Low‐temperature plasma on peri‐implant–related biofilm and gingival tissue

Carreiro

Délben²,

Guedes

et al. 2018

Journal of Periodontology

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Background Evaluate the effect of low‐temperature plasma (LTP) on an anaerobic biofilm and on the biological response of an in vitro reconstituted gingival epithelium tissue. Methods Porphyromonas gingivalis W83 biofilm was cultured on titanium discs and reconstituted gingival tissues were submitted to similar treatment conditions. Treatments: LTP1—plasma treatment for 1 minute, LTP3—plasma treatment for 3 minute, CHX—0.2% chlorhexidine for 1 minute, GAS—gas only (no plasma) for 3 minute, and NEGATIVE—no treatment. TRITON group was included as a positive control for tissue analysis. Counting of viable colony forming units (CFU/mL) and confocal laser scanning microscopy were performed to evaluate LTP's antimicrobial effect. EpiGingival tissue was evaluated through cytotoxocity, viability, histology, and imunnohistochemistry (Ki67, vascular endothelial growth factor‐A vascular endothelial growth factor A [VEGF‐A], and terminal deoxynucleotidyl transferase dUTP nick end labeling terminal deoxynucleotidyl transferase dutp nick end labeling [TUNEL] expression). Results LTP1 and LTP3 presented significantly different reduced CFU/mL reduction in comparison to the negative control (Ρ < 0.001), but it was not as effective as the positive control (CHX). Low cytotoxicity and high viability were observed in gingival epithelium of NEGATIVE, GAS, CHX, and both LTP groups. The morphologic analysis of gingival epithelium revealed minor cell damage in the plasma groups (score 1). LTP1, LTP3, GAS, and NEGATIVE groups exhibited less than 5% of basal layer positive cells. LTP1, LTP3, GAS, and CHX groups were not positive for TUNEL assay. LTP1 and LTP3 showed the most positivity for VEGF. Conclusions LTP treatment can be considered as an effective method for reducing P. gingivalis biofilm on implant surfaces, while being safe for the gingival epithelium. Furthermore, plasma treatment may be associated with cell repair.

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Perspectives in Dental Implantology

Jablonowski

Matthes

Duske³

et al. 2018

Comprehensive Clinical Plasma Medicine

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Osteoblast growth, after cleaning of biofilm‐covered titanium discs with air‐polishing and cold plasma

Abstract: An AP treatment has the potential to remove biofilm from rough implant surfaces completely. In contrast to our hypothesis, the combination of plasma and AP treatment did not enhance osteoblast spreading.

Cited by 37 publications

References 44 publications

Effects of air abrasive decontamination on titanium surfaces: A systematic review of in vitro studies

Effects of air abrasive decontamination on titanium surfaces: A systematic review of in vitro studies

Low‐temperature plasma on peri‐implant–related biofilm and gingival tissue

Perspectives in Dental Implantology

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