Osteoblast-like cell (MC3T3-E1) proliferation on bioerodible polymers: an approach towards the development of a bone-bioerodible polymer composite material

Elgendy, Hoda M.; Norman, Maria E.; Keaton, Altorous R.; Laurencin, Cato T.

doi:10.1016/0142-9612(93)90116-j

Cited by 120 publications

(63 citation statements)

References 35 publications

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“…It can be observed that more cells attached and more protruding cellular processes were developed for connection between adjacent cells on the HA-containing surfaces. The present observation is in agreement with MC3T3-E1 cell behavior on the surfaces of other polymer/HA composites [42]. Because the attachment of anchorage-dependent cells such as osteoblasts involves short-term events like physicochemical linkages between cells and materials, surface properties of materials including surface rigidity, surface roughness from topology, and surface chemistry strongly affect the quality of attachment [43].…”

Section: Hydrophilicity and Protein Adsorptionsupporting

confidence: 77%

“…Proteins such as fibronectin or vitronectin have been known to promote in vitro cell adhesion, particularly osteoblast [43]. These features have been reported from protein adsorption measurements on the surface of other substrates or polymer/calcium phosphate nanocomposites using human serum or FBS in many previous efforts [10,14,42,47,48]. Recently, Woo et al [49] showed more amount of adsorbed specific serum proteins such albumin, fibronectin, and vitronectin as well as higher DNA content of MC3T3-E1 cells cultured on poly(L-lactic acid)(PLLA)/HA composite scaffolds than on PLLA scaffolds only.…”

Section: In Vitro Cell Studiesmentioning

confidence: 86%

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Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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A series of crosslinkable nanocomposites has been developed using hydroxyapatite (HA) nanoparticles and poly(propylene fumarate) (PPF). PPF/HA nanocomposites with four different weight fractions of HA nanoparticles have been characterized in terms of thermal and mechanical properties. To assess surface chemistry of crosslinked PPF/HA nanocomposites, their hydrophilicity and capability of adsorbing proteins have been determined using static contact angle measurement and MicroBCA protein assay kit after incubation with 10% fetal bovine serum (FBS), respectively. In vitro cell studies have been performed using MC3T3-E1 mouse pre-osteoblast cells to investigate the ability of PPF/HA nanocomposites to support cell attachment, spreading, and proliferation after 1, 4, and 7 days. By adding HA nanoparticles to PPF, the mechanical properties of crosslinked PPF/ HA nanocomposites have not been increased due to the initially high modulus of crosslinked PPF. However, hydrophilicity and serum protein adsorption on the surface of nanocomposites have been significantly increased, resulting in enhanced cell attachment, spreading, and proliferation after 4 days of cell seeding. These results indicate that crosslinkable PPF/HA nanocomposites are useful for hard tissue replacement because of excellent mechanical strength and osteoconductivity.

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Section: Hydrophilicity and Protein Adsorptionsupporting

confidence: 77%

Section: In Vitro Cell Studiesmentioning

confidence: 86%

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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“…In particular, PLGA has been used extensively in the biomaterial field and is generally considered to be biocompatible. Additionally, the degradation rate of PLGA used as a scaffold can be controlled through an appropriate choice of the lactideto-glycolide ratio in the polymer 7,8) . Generally cells, especially stem cells included iPS cells, do not inherently attach to any surface -there must be some surface functionalization to promote adhesion.…”

Section: Introductionmentioning

confidence: 99%

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

et al. 2017

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In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.

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“…In tissue engineering, cells cultured on biomaterial surfaces provide a simple method for screening the biocompatibility of materials prior to testing in vivo testing 1,2 . Pore size, shape, porosity and interconnectivity are important characteristics of scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Lithograph-moulded poly-L-co-D,L lactide porous membranes for osteoblastic culture

et al. 2013

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Pore size, shape, wall morphology, porosity, and interconnectivity are important characteristics of the scaffolds. Lithography is a manufacturing technique that allows the production of tridimensional scaffolds with a controllable and reproducible inner architecture. The aim of this study was to use lithography to create a poly-L-co-D,L lactide (PLDLA) scaffold with symmetrical pore size and distribution, and to evaluate its biocompatibility with osteoblasts in vitro. Lithographic moulds were used to produce porous PLDLA membranes by a casting procedure. Osteoblasts were removed from calvarial bones and seeded onto porous and smooth PLDLA membranes after which cell viability and adhesion assays, cytochemical analysis and scanning electron microscopy were used to characterize the cells. Cell viability and adhesion assays, cytochemical analysis, and scanning electron microscopy were carried out. Cell viability was similar on porous and smooth PLDLA membranes but higher than on a polystyrene substrate (positive control). Although osteoblasts adhered to the surface of all the materials tested, cell adhesion to lithographed PLDLA was greater than to smooth PLDLA membranes. In conclusion, osteoblasts interacted well with PLDLA membranes, as shown by the viability and adhesion assays and by the enhanced collagen production.

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Osteoblast-like cell (MC3T3-E1) proliferation on bioerodible polymers: an approach towards the development of a bone-bioerodible polymer composite material

Cited by 120 publications

References 35 publications

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

Lithograph-moulded poly-L-co-D,L lactide porous membranes for osteoblastic culture

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