Osteoblastic cell response on fluoridated hydroxyapatite coatings: the effect of magnesium incorporation

Cai, Yanli; Zhang, J J; Zhang, Songbai; Venkatraman, Subbu S.; Zeng, Xin; Du, Hejun; Mondal, Debasish

doi:10.1088/1748-6041/5/5/054114

Cited by 60 publications

(43 citation statements)

References 23 publications

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“…In this case, as calcium phosphate particles, they also participated to the precipitation of apatite-like crystals onto the scaffold surface from the bulk. However, the presence of the bioactive coating did not cover the bioactive signal of MgCHA particles, which was mediated by the release of Mg, Ca and P ions to cells as reported in previous study 29 …”

Section: Discussionmentioning

confidence: 61%

Bioactivity and bone healing properties of biomimetic porous composite scaffold:In vitroandin vivostudies

Veronesi

Giavaresi

Guarino

et al. 2015

J Biomedical Materials Res

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Tissue engineering (TE) represents a valid alternative to traditional surgical therapies for the management of bone defects that do not regenerate spontaneously. Scaffolds, one of the most important component of TE strategy, should be biocompatible, bioactive, osteoconductive, and osteoinductive. The aim of this study was to evaluate the biological properties and bone regeneration ability of a porous poly(ɛ-caprolactone) (PCL) scaffold, incorporating MgCO3 -doped hydroxyapatite particles, uncoated (PCL_MgCHA) or coated by apatite-like crystals via biomimetic treatment (PCL_MgCHAB). It was observed that both scaffolds are not cytotoxic and, even if cell viability was similar on both scaffolds, PCL_MgCHAB showed higher alkaline phosphatase and collagen I (COLL I) production at day 7. PCL_MgCHA induced more tumor necrosis factor-α release than PCL_MgCHAB, while osteocalcin was produced less by both scaffolds up to 7 days and no significant differences were observed for transforming growth factor-β synthesis. The percentage of new bone trabeculae growth in wide defects carried out in rabbit femoral distal epiphyses was significantly higher in PCL_MgCHAB in comparison with PCL_MgCHA at 4 weeks and even more at 12 weeks after implantation. This study highlighted the role of a biomimetic composite scaffold in bone regeneration and lays the foundations for its future employment in the clinical practice.

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Section: Discussionmentioning

confidence: 61%

Bioactivity and bone healing properties of biomimetic porous composite scaffold:In vitroandin vivostudies

Veronesi

Giavaresi

Guarino

et al. 2015

J Biomedical Materials Res

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“…Firstly, surface treatment with inorganic ions induces a significant roughness modification which is responsible, in turn, of an increased cell adhesion and of a reduced osteoblasts cytotoxicity, as demonstrated in our previous paper 9) . Furthermore, it can be argued that magnesium and calcium addition on test surface plays a pivotal role in promoting and enhancing cell differentiation thus improving bone-implant contact, as supported by the scientific literature 21,22,24) . However the reasons of this outcome still remain unknown and need to be clarified in future studies.…”

Section: Discussionmentioning

confidence: 93%

“…Moreover, as the scientific literature widely demonstrated some inorganic ions, such as magnesium and calcium, are able to stimulate hard tissue regeneration by promoting osteoblasts proliferation and differentiation 21,22) . It was hypothesized that the addition of calcium and magnesium inorganic ions (CaMg) to the implant surface could increase the levels of PGE2 and the expression of BMP2 and RUNX2, generally considered osteoblastic differentiation markers.…”

Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

et al. 2018

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Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite, (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.

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“…Previous studies demonstrated similar results where cells on Mg containing surfaces showed higher proliferation and differentiation compared to Mg-free specimens. 30,38,39 Furthermore, Mg ions are considered to be beneficial for bone cell attachment. Zreiqat et al 23 reported that cells grown on Mg-modified alumina exhibited a significantly increased adhesion since binding of integrins to specific ligands depends on the presence of divalent cations.…”

Section: Discussionmentioning

confidence: 99%

Magnesium-containing mixed coatings on zirconia for dental implants: mechanical characterization and in vitro behavior

et al. 2015

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An important challenge in the field of dental and orthopedic implantology is the preparation of implant coatings with bioactive functions that feature a high mechanical stability and at the same time mimic structural and compositional properties of native bone for a better bone ingrowth. This study investigates the influence of magnesium addition to zirconia-calcium phosphate coatings. The mixed coatings were prepared with varying additions of either magnesium oxide or magnesium fluoride to yttria-stabilized zirconia and hydroxyapatite. The coatings were deposited on zirconia discs and screw implants by wet powder spraying. Microstructure studies confirm a porous coating with similar roughness and firm adhesion not hampered by the coating composition. The coating morphology, mechanical flexural strength and calcium dissolution showed a magnesium content-dependent effect. Moreover, the in vitro results obtained with human osteoblasts reveal an improved biological performance caused by the presence of Mg 2þ ions. The magnesiumcontaining coatings exhibited better cell proliferation and differentiation in comparison to pure zirconia-calcium phosphate coatings. In conclusion, these results demonstrate that magnesium addition increases the bioactivity potential of zirconia-calcium phosphate coatings and is thus a highly suitable candidate for bone implant coatings.

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Osteoblastic cell response on fluoridated hydroxyapatite coatings: the effect of magnesium incorporation

Cited by 60 publications

References 23 publications

Bioactivity and bone healing properties of biomimetic porous composite scaffold:In vitroandin vivostudies

Bioactivity and bone healing properties of biomimetic porous composite scaffold:In vitroandin vivostudies

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

Magnesium-containing mixed coatings on zirconia for dental implants: mechanical characterization and in vitro behavior

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