Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates

Ciapetti, G.; Pompo, Gemma Di; Avnet, Sofia; Martini, Désirèe; Díez-Escudero, Anna; Montúfar, Edgar B.; Ginebra, Maria‐Pau; Baldini, Nicola

doi:10.1016/j.actbio.2016.12.013

Cited by 44 publications

(36 citation statements)

References 60 publications

(70 reference statements)

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“…This agrees with our previous findings where prolonged exposure to cell culture medium did not affect the surface morphology of plate-like CDHA. [60] Higher levels of CAR2 and MMP9 were found when the cells were in contact with C substrates compared to their F counterparts with the same L/P ratio (C35 vs F35 and C65 an F65),…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 91%

“…indicating that greater surface roughness may trigger some degradative activity. [60,61] Another sign of degradative behaviour was the higher ion concentration, especially calcium, in the cell culture media in presence of macrophages compared to the cell-free substrates ( Figure 4D). In fact, the release of these ions into the medium can be associated to the dissolution of CDHA by the activity of immune cells, which counteracts the ionic exchange produced by the material itself, similarly to what was found in a previous study.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

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Effect of nano-structural properties of biomimetic hydroxyapatite on osteoimmunomodulation

et al. 2018

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Immune cells are sensitive to the microstructural and textural properties of materials. Tuning the structural features of synthetic bone grafts could be a valuable strategy to regulate the specific response of the immune system, which in turn modulates the activity of bone cells. The aim of this study was to analyse the effect of the structural characteristics of biomimetic calcium deficient hydroxyapatite (CDHA) on the innate immune response of macrophages and the subsequent impact on osteogenesis and osteoclastogenesis. Murine RAW 264.7 cells were cultured, under standard and inflammatory conditions, on chemically identical CDHA substrates that varied in microstructure and porosity. The impact on osteogenesis was evaluated by incubating osteoblastic cells (SaOS-2) with RAW-CDHA conditioned extracts. The results showed that macrophages were sensitive to different textural and structural properties of CDHA. Under standard conditions, the impact of inflammatory cytokine production by RAW cells cultured on CDHA played a significant role in the degradation of substrates, suggesting the impact of resorptive behaviour of RAW cells on biomimetic surfaces. Osteoblast differentiation was stimulated by the conditioned media collected from RAW cells cultured on needle-like nanostructured CDHA. The results demonstrated that needle-like nanostructured CDHA was able to generate a favourable osteoimmune environment to regulate osteoblast differentiation and osteogenesis. Under inflammatory conditions, the incubation of RAW cells with less porous CDHA resulted in a decreased gene expression and release of pro-inflammatory cytokines.

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Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 91%

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Effect of nano-structural properties of biomimetic hydroxyapatite on osteoimmunomodulation

et al. 2018

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“…Osteogenic differentiation of human mesenchymal stem cells is stimulated by reduced graphene oxide level. Osteoclast differentiation from human blood precursors can be enhanced by using biomimetic calcium-phosphate substrates [44]. Insulintransferrin-selenium is also play important role in auricular chondrocyte proliferation and engineered cartilage formation in vitro [45].…”

Section: Role Of Mineral Elements In Osteogenic Differentiationmentioning

confidence: 99%

Role of Calcium Bio-Minerals in Regenerative Medicine and Tissue Engineering

Upadhyay¹

2017

JSRT

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Present review article emphasize role of biominerals in regenerative medicine and tissue engineering. Among all biominerals calcium is essential for body growth and development. It also performs many fundamental functions in cellular metabolism. Inside cell organic matrix is calcified by calcium phosphate minerals. It also embeds bone cells which participate in the maintenance and organization of bone. This article also emphasizes use of hydroxyapatite a natural mineral used as a bone-building supplement with superior absorption in comparison to calcium. It also explains use of scaffolds that mimic the structure and composition of bone tissue and cells. It also signifies use of HAc microparticles or microparticles loaded with PL, superparamagnetic iron oxide nanoparticles, composite scaffolds of nano-hydroxyapatite (nHAp) and silk fibroin (SF) in bone regeneration mainly in osteoregenerative therapy. For better and successful bone regeneration there is a need to develop low cost sintered hydroxyfluorapatite discs to support cellular proliferation and colonization, tailored mineralization, cell and drug delivery. All adhesion components should show low immunoreactivity and high biocompatibility with natural bone tissues. There is an essential need to make new biocompatible materials for scaffolding, biomeinerals and cementing formulations for regeneration of bones, craniomaxillofacial, dental and orthopedic surgery.

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“…The investigation reveals that the bone graft fusion rate is a significant index to assess bone‐filling materials in pre‐clinical animal model tests, and qualitatively to assess the situation of fusion degree between the original bone and the applied product. The experimental design should cover the steps of the process in detail, including the selection of animals, anesthesia, acquisition of the related bone parts, sectioning, dyeing, and microscopic observation.…”

Section: Pre‐clinical Animal Model Testmentioning

confidence: 99%

Pre‐Registration Assessment of Bone‐Filling Products

Pan

Liu

Yang

et al. 2019

Orthopaedic Surgery

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The use of bone‐filling material to repair bone defects and fix implanted bone grafts is a developing area in medicine. Investigators can evaluate bone‐filling materials through use of several indices to make comparisons and to determine suitability for application in humans1. However, it is quite difficult to transform their discovery into practical use, because the viability of the studied material might require examination of all aspects of properties. In addition, for a material to become a product, a complete procedure involving a declaration, registration, and approval is necessary. This article introduces the technical indices that the investigators and reporters should provide in their declaration and registration data to meet the relevant standards in China. The indices include physical and chemical properties, biocompatibility, biosecurity, pre‐clinical animal model tests, sterilization and disinfection, product duration, and packaging. Full consideration of all possible indices is crucial to realize the transformation from a designed product to a commercial medical device, which requires effective interaction between clinicians and engineers.

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Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates

Cited by 44 publications

References 60 publications

Effect of nano-structural properties of biomimetic hydroxyapatite on osteoimmunomodulation

Effect of nano-structural properties of biomimetic hydroxyapatite on osteoimmunomodulation

Role of Calcium Bio-Minerals in Regenerative Medicine and Tissue Engineering

Pre‐Registration Assessment of Bone‐Filling Products

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