Osteogenic activity of nanonized pearl powder/poly (lactide-co-glycolide) composite scaffolds for bone tissue engineering

Yang, Yueh-Lung; Chang, Chiung‐Wen; Huang, Ching-Cheng; Kao, Wenny Mei-Wen; Liu, Wei-Chung; Liu, Hsia‐Wei

doi:10.3233/bme-130893

Cited by 10 publications

(4 citation statements)

References 10 publications

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“…Therefore, many attempts have been made to combine synthetic with natural materials. Recently, poly( d , l -lactide- co -glycolide) has been combined with a naturally bioceramic hybrid material, nanonized pearl powder, as an osteoinductive material: the scaffold was able to influence osteoblast behavior in vitro [94]. The benefits associated with polyhydroxyalkanoates (PHA) in tissue engineering include high immunotolerance, low toxicity, and biodegradability.…”

Section: Structure and Properties Of Grafts And Bone Substitutesmentioning

confidence: 99%

Bone regenerative medicine: classic options, novel strategies, and future directions

et al. 2014

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This review analyzes the literature of bone grafts and introduces tissue engineering as a strategy in this field of orthopedic surgery. We evaluated articles concerning bone grafts; analyzed characteristics, advantages, and limitations of the grafts; and provided explanations about bone-tissue engineering technologies. Many bone grafting materials are available to enhance bone healing and regeneration, from bone autografts to graft substitutes; they can be used alone or in combination. Autografts are the gold standard for this purpose, since they provide osteogenic cells, osteoinductive growth factors, and an osteoconductive scaffold, all essential for new bone growth. Autografts carry the limitations of morbidity at the harvesting site and limited availability. Allografts and xenografts carry the risk of disease transmission and rejection. Tissue engineering is a new and developing option that had been introduced to reduce limitations of bone grafts and improve the healing processes of the bone fractures and defects. The combined use of scaffolds, healing promoting factors, together with gene therapy, and, more recently, three-dimensional printing of tissue-engineered constructs may open new insights in the near future.

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Section: Structure and Properties Of Grafts And Bone Substitutesmentioning

confidence: 99%

Bone regenerative medicine: classic options, novel strategies, and future directions

et al. 2014

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“…Any loss or damage in the jaws or teeth was one of the major health problems that the humanity faced upon the early ages. Due to its accessibility, the calcium phosphates-based materials were the first choice in the repair and restoration of jawbones [14]. Initial application of the raw material was not practical due to the powder form.…”

Section: The Relevance Of the Biodegradation In Oral Reconstructionmentioning

confidence: 99%

Biodegradation of Injectable Calcium Phosphate Bone Cements: A Dental Perspective

Arısan¹

2016

Dental Implantology and Biomaterial

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The prevalence of tooth loss and edentulism is among the most ubiquitous diseases of human history. Dental implants have been widely used for the treatment of any type of edentulism. However, the absence of sufficient bone volume usually limits the placement of implants. Many techniques and materials have been developed to restore an adequate volume of bone for future implant placement, but the process of biodegradation and replacement with new bone tissue is still under debate for all grafting materials. Among the available biomaterials, calcium phosphates (CaP) have been under the spot light for their advantages such as the ease of production and lack of disease transfer. The preparation of the material in two phases allowed self-hardening and subsequent space maintenance where it applied. This was of critical importance in load-bearing implants and joint prosthesis where rapid and strong healing is required. The injectability also allowed a better handling and manipulation in compromised areas such as the oral cavity. A novel form of injectable calcium phosphate cement (iCaP) with two distinct formulations was tested on dog tibiae. Healing and ossification at 4 and 12 weeks were assessed by histologic and histomorphometric analysis. No adverse reactions or negative consequences were noted. Mean new bone formation was 22.12 (SD, 15.68), 18.62 (SD, 13.11), and 9.56 (SD, 11.11)% in the groups 1, 2, and the control, respectively. Statistically, significant higher new bone formation was evident in the groups 1 and 2 as compared to the control group (p < 0.01). However, these differences were no more discernable after 12 weeks of healing. The results of the present investigation showed excellent in vivo biocompatibility but insufficient biodegradation of the iCaP in the center of the defect area. Further attempts are required to expedite the biodegradation of the iCaP.

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“…The pearl/PLGA scaffold promoted the ALP activity, collagen I (Col-I) synthesis in marrow stem cells (MSCs) in vitro compared to PLGA/TCP scaffold [ 28 ]. Yang et al reported that PLGA/pearl composite scaffold fabricated by gel casting and salt leaching method could promote the growth of osteoblasts in vitro compared to PLGA control [ 29 ]. Zhang et al developed poly-caprolactone (PCL)/pearl scaffold using 3D printing technique.…”

Section: Introductionmentioning

confidence: 99%

Comparing the regeneration potential between PLLA/Aragonite and PLLA/Vaterite pearl composite scaffolds in rabbit radius segmental bone defects

Huang

Liu

Ouyang

et al. 2020

Bioactive Materials

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Mussel-derived nacre and pearl, which are natural composites composed CaCO 3 platelets and interplatelet organic matrix, have recently gained interest due to their osteogenic potential. The crystal form of CaCO 3 could be either aragonite or vaterite depending on the characteristics of mineralization template within pearls. So far, little attention has been paid on the different osteogenic capacities between aragonite and vaterite pearl. In the current work, aragonite or vaterite pearl powders were incorporated into poly- l -lactic acid (PLLA) scaffold as bio-functional fillers for enhanced osteogenesis. In intro results revealed that PLLA/aragonite scaffold possessed stronger stimulatory effect on SaOS-2 cell proliferation and differentiation, evidenced by the enhanced cell viability, alkaline phosphatase activity, collagen synthesis and gene expressions of osteogenic markers including osteocalcin, osteopotin and bone sialoprotein. The bone regeneration potential of various scaffolds was evaluated in vivo employing a rabbit critical-sized radial bone defect model. The X-ray and micro-CT results showed that significant bone regeneration and bridging were achieved in defects implanted with composite scaffolds, while less bone formation and non-bridging were found for pure PLLA group. Histological evaluation using Masson's trichrome and hematoxylin/eosin (H&E) staining indicated a typical endochondral bone formation process conducted at defect sites treated with composite scaffolds. Through three-point bending test, the limbs implanted with PLLA/aragonite scaffold were found to bear significantly higher bending load compared to other two groups. Together, it is suggested that aragonite pearl has superior osteogenic capacity over vaterite pearl and PLLA/aragonite scaffold can be employed as a potential bone graft for bone regeneration.

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Osteogenic activity of nanonized pearl powder/poly (lactide-co-glycolide) composite scaffolds for bone tissue engineering

Cited by 10 publications

References 10 publications

Bone regenerative medicine: classic options, novel strategies, and future directions

Bone regenerative medicine: classic options, novel strategies, and future directions

Biodegradation of Injectable Calcium Phosphate Bone Cements: A Dental Perspective

Comparing the regeneration potential between PLLA/Aragonite and PLLA/Vaterite pearl composite scaffolds in rabbit radius segmental bone defects

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