Osteogenic Activity of the Fourteen Types of Human Bone Morphogenetic Proteins (Bmps)

Cheng, Hongwei; Jiang, Wei; Phillips, Frank M.; Haydon, Rex C.; Peng, Ying; Zhou, Lan; Luu, Hue H.; An, Naili; Breyer, Benjamin N.; Vanichakarn, Pantila; Szatkowski, Jan P.; Park, Sun-Hee; He, Tong‐Chuan

doi:10.2106/00004623-200308000-00017

Cited by 887 publications

(580 citation statements)

References 70 publications

Supporting

Mentioning

551

Contrasting

Unclassified

Order By: Relevance

“…Recombinant Adenoviral Vectors Expressing BMPs, Wnt3A, Oncogenic ␤-Catenin, and CTGF-Recombinant adenoviruses (AdBMPs) expressing human BMP-2, -6, and -9 (also known as GDF-2) were generated as described previously (11,50). For construction of the adenoviral vectors expressing Wnt3A, the coding region of mouse Wnt3A (kindly provided by Roel Nusse of Stanford University) was PCR-amplified and subcloned into pAdTrack-CMV, resulting in pAdTrack-Wnt3A.…”

Section: Methodsmentioning

confidence: 99%

“…Determination of Alkaline Phosphatase Activity-Alkaline phosphatase activity was assessed by the colorimetric assay and/or histochemical staining assay as described previously (11,12,15).…”

Section: Methodsmentioning

confidence: 99%

“…Stem Cells-Through a comprehensive analysis of the osteogenic activity of the 14 types of human BMPs, we demonstrated previously (11,12) that BMP-2, BMP-6, and BMP-9 exhibit the greatest ability to induce osteoblast differentiation of mesenchymal progenitor cells in vitro as well as in vivo. As shown in Fig.…”

Section: Expression Profiling Analysis Of the Early Stage Of Bmp And mentioning

confidence: 99%

“…In this study, we focused on BMP-9 as we have demonstrated that BMP-9 is one of the most potent osteogenic BMPs (11,12), yet it is one of the least characterized BMPs. As shown in Fig.…”

Section: Ctgf Is Among the Most Significantly Up-regulated Target Genmentioning

confidence: 99%

“…Genetic disruptions of BMPs have resulted in various skeletal and extraskeletal abnormalities during development (10). Through a comprehensive analysis of the osteogenic activity of the 14 types of human BMPs, we have recently demonstrated that BMP-2, BMP-6, and BMP-9 are the most potent inducers of osteogenic differentiation both in vitro and in vivo (11,12). BMPs fulfill their signaling activity by interacting with the heterodimeric complex of two transmembrane serine/threonine kinase receptors, BMPR type I and BMPR type II (13,14).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Connective Tissue Growth Factor (CTGF) Is Regulated by Wnt and Bone Morphogenetic Proteins Signaling in Osteoblast Differentiation of Mesenchymal Stem Cells

Luo

Kang

et al. 2004

Journal of Biological Chemistry

Self Cite

296

356

View full text Add to dashboard Cite

Osteoblast lineage-specific differentiation of mesenchymal stem cells is a well regulated but poorly understood process. Both bone morphogenetic proteins (BMPs) and Wnt signaling are implicated in regulating osteoblast differentiation and bone formation. Here we analyzed the expression profiles of mesenchymal stem cells stimulated with Wnt3A and osteogenic BMPs, and we identified connective tissue growth factor (CTGF) as a potential target of Wnt and BMP signaling. We confirmed the microarray results, and we demonstrated that CTGF was up-regulated at the early stage of BMP-9 and Wnt3A stimulations and that Wnt3A-regulated CTGF expression was ␤-catenin-dependent. RNA interference-mediated knockdown of CTGF expression significantly diminished BMP-9-induced, but not Wnt3A-induced, osteogenic differentiation, suggesting that Wnt3A may also regulate osteoblast differentiation in a CTGF-independent fashion. However, constitutive expression of CTGF was shown to inhibit both BMP-9-and Wnt3A-induced osteogenic differentiation. Exogenous expression of CTGF was shown to promote cell migration and recruitment of mesenchymal stem cells. Our findings demonstrate that CTGF is up-regulated by Wnt3A and BMP-9 at the early stage of osteogenic differentiation, which may regulate the proliferation and recruitment of osteoprogenitor cells; however, CTGF is down-regulated as the differentiation potential of committed pre-osteoblasts increases, strongly suggesting that tight regulation of CTGF expression may be essential for normal osteoblast differentiation of mesenchymal stem cells.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Determination of Alkaline Phosphatase Activity-Alkaline phosphatase activity was assessed by the colorimetric assay and/or histochemical staining assay as described previously (11,12,15).…”

Section: Methodsmentioning

confidence: 99%

Section: Expression Profiling Analysis Of the Early Stage Of Bmp And mentioning

confidence: 99%

“…In this study, we focused on BMP-9 as we have demonstrated that BMP-9 is one of the most potent osteogenic BMPs (11,12), yet it is one of the least characterized BMPs. As shown in Fig.…”

Section: Ctgf Is Among the Most Significantly Up-regulated Target Genmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Connective Tissue Growth Factor (CTGF) Is Regulated by Wnt and Bone Morphogenetic Proteins Signaling in Osteoblast Differentiation of Mesenchymal Stem Cells

Luo

Kang

et al. 2004

Journal of Biological Chemistry

Self Cite

296

356

View full text Add to dashboard Cite

show abstract

EGR1 plays an important role in BMP9‐mediated osteoblast differentiation by promoting SMAD1/5 phosphorylation

et al. 2022

View full text Add to dashboard Cite

Bone morphogenetic proteins (BMPs) are essential regulators of skeletal homeostasis, and BMP9 is the most potently osteogenic among them. Here, we found that BMP9 and BMP2 rapidly induced early growth response 1 (EGR1) protein expression in osteoblasts through MEK/ERK pathway‐dependent transcriptional activation. Knock‐down of EGR1 using siRNA significantly inhibited BMP9‐induced matrix mineralization and osteogenic marker gene expression in osteoblasts. Knock‐down of EGR1 significantly reduced SMAD1/5 phosphorylation and inhibited the expression of their transcriptional targets in osteoblasts stimulated by BMP9. In contrast, forced EGR1 overexpression in osteoblasts enhanced BMP9‐mediated osteoblast differentiation and SMAD1/5 phosphorylation. An intracellular association between EGR1 and SMAD1/5 was identified using immunoprecipitation assays. These results indicated that EGR1 plays an important role in BMP9‐stimulated osteoblast differentiation by enhancing SMAD1/5 phosphorylation.

show abstract

Regenerative Osseous Surgery: The Use of Growth Factor‐Enhanced Bone Grafts

Schulze‐Späte¹,

Kayal²

2011

Practical Osseous Surgery in Periodontics and Implant Dentistry

View full text Add to dashboard Cite

Osteogenic Activity of the Fourteen Types of Human Bone Morphogenetic Proteins (Bmps)

Cited by 887 publications

References 70 publications

Connective Tissue Growth Factor (CTGF) Is Regulated by Wnt and Bone Morphogenetic Proteins Signaling in Osteoblast Differentiation of Mesenchymal Stem Cells

Connective Tissue Growth Factor (CTGF) Is Regulated by Wnt and Bone Morphogenetic Proteins Signaling in Osteoblast Differentiation of Mesenchymal Stem Cells

EGR1 plays an important role in BMP9‐mediated osteoblast differentiation by promoting SMAD1/5 phosphorylation

Regenerative Osseous Surgery: The Use of Growth Factor‐Enhanced Bone Grafts

Contact Info

Product

Resources

About