Osteogenic and chondrogenic differentiation effect of neohesperidin dihydrochalcones in human adipose‐derived stem cells

Abstract

Reactive oxygen species (ROS) are highly reactive which cause damage to various cellular functions such as proliferation, differentiation, and survival. Nuclear factor erythroid 2‐related factor‐2 (Nrf2) promotes expression of antioxidant enzyme such as heme oxygenase 1 (HO‐1) and NAD(P)H:Quinone Oxidoreductase 1 (NQO‐1). Neohesperidin is a compound originated from peel of citrus fruits and its hydrogenated form, neohesperidin dihydrochalcone (NHDC) has antioxidant activity. Human adipose‐derived stem cells (hADSC) are multipotent adult stem cells responsible for the regeneration of fat, bone and cartilage. Thus, we observed the antioxidant effects of NHDC derivatives on hADSC through Nrf2 activation. Upregulation of ROS inhibits osteogenic differentiation of hADSCs, in part through inhibition of signaling pathway, which is essential for bone development and maintenance. ROS levels regulate inhibition of proliferation and modulate initiation of the hypertrophic changes in chondrocytes. Cellular viability and compounds' proper concentrations were set by MTS assay. The effects of these compounds on suppressing oxidative stress and hADSCs differentiation into osteocytes and chondrocytes was investigated using Alizarin Red staining, Alcian Blue staining, reverse‐transcriptase polymerase chain reaction (RT‐PCR), and Western blotting. The results demonstrate that NHDC derivatives play an efficacious role in inducing osteogenic and chondrogenic differentiation of hADSCs through reducing cellular oxidative stress.Support or Funding InformationThis research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education 2018R1D1A1B07049077This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.