Osteogenic differentiation of human adipose-derived mesenchymal stem cells on gum tragacanth hydrogel

Haeri, Seyed Mohammad Jafar; Sadeghi, Yousef; Salehi, Mohammad Saied; Farahani, Reza Masteri; Nourozian, Mohsen

doi:10.1016/j.biologicals.2016.03.004

Cited by 32 publications

(15 citation statements)

References 31 publications

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“…Given the demonstrated safety of this biomaterial, the use of scaffolds alone could represent a safe and promising approach for bone healing, without additional use of growth factors. The use of autologous hADSCs in combination with the scaffolds represents a feasible and promising clinical choice, and some recent studies confirm our findings, although in combination with biomaterials different from that used here 26 – 29 . In particular, the use of hADSCs along with scaffolds would be promising especially for elderly individuals or other particular groups of patients with reduced regenerative capacity.…”

Section: Discussionsupporting

confidence: 85%

Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse

Calabrese

Giuffrida²,

Forte³

et al. 2017

Sci Rep

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Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of inducing the right commitment of cells seeded within the scaffold. In particular, given their properties and large availability, the human adipose-derived stem cells are considered as the better candidate for autologous cell transplantation. In order to evaluate the regenerative potential of these cells along with an osteoinductive biomaterial, we have used collagen/hydroxyapatite scaffolds to test ectopic bone formation after subcutaneous implantation in mice. The process was analysed both in vivo, by Fluorescent Molecular Tomography (FMT), and ex vivo, to evaluate the formation of bone and vascular structures. The results have shown that the biomaterial could itself be able of promoting differentiation of host cells and bone formation, probably by means of its intrinsic chemical and structural properties, namely the microenvironment. However, when charged with human mesenchymal stem cells, the ectopic bone formation within the scaffold was increased. We believe that these results represent an important advancement in the field of bone physiology, as well as in regenerative medicine.

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Section: Discussionsupporting

confidence: 85%

Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse

Calabrese

Giuffrida²,

Forte³

et al. 2017

Sci Rep

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“…Perfusion systems have been proven to be beneficial for tissue engineering purposes such as bone or cartilage engineering [ 36 , 37 ]. For instance, numerous studies show a higher matrix deposition or upregulation of relevant genes in comparison to static culture conditions when bone precursor cells are exposed to fluid shear stress [ 38 , 39 , 40 , 41 , 42 , 43 ]. Although several tailor-made [ 10 , 11 , 44 ] and commercially available [ 45 , 46 , 47 ] perfusion systems were developed and successfully used, there is a lack of automated sensor-controlled systems that allow the cultivation of multiple independent replicates under different conditions.…”

Section: Discussionmentioning

confidence: 99%

Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture

et al. 2017

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The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, we developed a miniaturized, parallelizable perfusion bioreactor system with two different bioreactor chambers. Pressure sensors were also implemented to determine the permeability of biomaterials which allows us to approximate the shear stress conditions. To characterize the flow velocity and shear stress profile of a porous scaffold in both bioreactor chambers, a computational fluid dynamics analysis was performed. Furthermore, the mixing behavior was characterized by acquisition of the residence time distributions. Finally, the effects of the different flow and shear stress profiles of the bioreactor chambers on osteogenic differentiation of human mesenchymal stem cells were evaluated in a proof of concept study. In conclusion, the data from computational fluid dynamics and shear stress calculations were found to be predictable for relative comparison of the bioreactor geometries, but not for final determination of the optimal flow rate. However, we suggest that the system is beneficial for parallel dynamic cultivation of multiple samples for 3D cell culture processes.

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“…Further, compared to tissue culture plate (TCPS) and collagen hydrogel, GT hydrogel exhibited the highest mineralization and ALP activity with adipose-derived MSCs (ADMSCs). Relative gene expression levels exhibited the highest expression of Runx2, osteonectin, and osteocalcin in GT hydrogel cultured with cells, but the expression of collagen type I remains constant [164]. The incorporation of cellulose nanocrystals (CNCs) in XG/silica glass (SG) scaffolds showed tunable and improved mechanical properties with good pre-osteoblast MC3T3-E1 cells cytocompatibility [165].…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

“…Supporting and the acceleration of adhesion, proliferation, and osteogenic differentiation of stem cells [164] GT PVA, glutaraldehyde (crosslinker) Wound healing human fibroblast AGO cells Good antibacterial properties against Gram-negative bacteria and cell adhesion and proliferation [137] GT PCL, Cur Wound healing Mesenchyme stem cells (MSCs)…”

Section: Other Tissue Engineeringmentioning

confidence: 99%

Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review

et al. 2020

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The engineering of tissues under a three-dimensional (3D) microenvironment is a great challenge and needs a suitable supporting biomaterial-based scaffold that may facilitate cell attachment, spreading, proliferation, migration, and differentiation for proper tissue regeneration or organ reconstruction. Polysaccharides as natural polymers promise great potential in the preparation of a three-dimensional artificial extracellular matrix (ECM) (i.e., hydrogel) via various processing methods and conditions. Natural polymers, especially gums, based upon hydrogel systems, provide similarities largely with the native ECM and excellent biological response. Here, we review the origin and physico-chemical characteristics of potentially used natural gums. In addition, various forms of scaffolds (e.g., nanofibrous, 3D printed-constructs) based on gums and their efficacy in 3D cell culture and various tissue regenerations such as bone, osteoarthritis and cartilage, skin/wound, retinal, neural, and other tissues are discussed. Finally, the advantages and limitations of natural gums are precisely described for future perspectives in tissue engineering and regenerative medicine in the concluding remarks.

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Osteogenic differentiation of human adipose-derived mesenchymal stem cells on gum tragacanth hydrogel

Cited by 32 publications

References 31 publications

Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse

Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse

Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture

Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review

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