Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: A surface-roughness gradient study

Abstract

Biodegradable polymers, such as polycaprolactone (PCL), are promising materials in the field of tissue engineering and regenerative medicine, which aims at creating viable options to replace permanent orthopedic implants. The material, cells, and growth-stimulating factors are often referred to as the key components of engineered tissues. In this article, we studied the hypothesis of specific surface modification of PCL being capable of inducing mesenchymal stem cell differentiation in bone cells in the absence of cell-differentiating factors. The systematic investigation of the linearly varying surface-roughness gradient showed that an average PCL roughness of 0.93 μm alone can serve as a compelling alternative to soluble osteogenic inducers in orthopedic applications featuring the clinically relevant biodegradable polymer polycaprolactone.

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

“…In addition, the scaffolds with lower surface roughness could induce better cell proliferation and chondrogenic differentiation after 7 days of culture . Faia‐Torres et al showed that PCL scaffolds with roughness of 0.93 µm could induce osteogenic differentiation in hMSCs ( Figure ) . Human coronary artery cells were tested on the smooth and rough surfaces using PLLA scaffolds.…”

Microfluidic‐Printed Microcarrier for In Vitro Expansion of Adherent Stem Cells in 3D Culture Platform

“…Surface modification of the 2.5D microcarrier by PDA is advantageous in that it is an animal‐free uniform coating method that provides reproducible results, regardless of the reaction scale. As the PDA pigmentation increased, the surface roughness also increased (Figure S2, Supporting Information), thereby promoting cell attachment; however, the increased PDA pigmentation can potentially hinder observations of the cell morphology.…”

Comparative Assessment of the Possibility of Cellular Material Collection to Matrixes Obtained by Methods of Electrospinning and Aerodynamic Formation in a Turbulent Gas Flow

“…Такой результат, по всей видимости, обусловлен более высоким значением площади свобод-ной поверхности. Кроме того, известно, что поверхности с большей шероховатостью являются предпочтительны-ми для адгезии клеток [16], можно предположить, что развитая поверхность волокон, сформированных мето-дом АЭРДФ, стимулирует прикрепление клеток. Также на более эффективное заселение клеток в матрикс № 2 мог-ло повлиять наличие в его структуре свободных частиц фосфатов кальция, так как насыщение жидкости вокруг частиц и порового пространства матрикса биологически активными ионами кальция и фосфора оказывает синер-гетический эффект и усиливает адгезию.…”