Osteogenic differentiation of mesenchymal stem cells cultured on allogenic trabecular bone grafts treated with high hydrostatic pressure

Abstract

The requirements for bone substitute materials are multifaceted. Beside biomechanical stability, these materials should provide osteoconductive and osteoinductive properties to promote integration into the host tissue. So far, autologous bone is the only material, which combines all properties, but is naturally limited. Allogenic bone grafts have to be decellularized prior to implantation. This causes the reduction of biomechanical properties and the loss of osteoinductive qualities. High hydrostatic pressure (HHP) offers a gentle alternative for processing and supply of allogenic bone substitute materials while preserving biomechanical integrity. To determine whether osteogenic properties are retained by HHP treatment, mesenchymal stem cells (MSCs) were cultured with HHP‐treated and untreated allogenic trabecular bone blocks up to 28 days. Both, gene expression and protein analysis showed that HHP‐treated bone positively influenced differentiation of MSCs into osteoblasts and mineralization of bone matrix. This effect was greater in samples cultivated with HHP‐treated bone blocks. The present study shows that HHP treatment does not result in the reduction of osteoinductivity, thus serving as an alternative approach for processing allogeneic bone substitute materials.

Systematic enhancement of microbial decontamination efficiency in bone graft processing by means of high hydrostatic pressure using Escherichia coli as a model organism

“…30,32 This may also include various cell-graft interaction studies, such as colonization with stem cells or osteoblasts. 71,72 Compared to conventional bone sterilization protocols, HHP…”

“…Once the process parameters that are required to ensure effective decontamination are established, biomechanical integrity and immunological safety of the sterilized implants have to be characterized, since process parameters might vary from previous studies 30,32 . This may also include various cell‐graft interaction studies, such as colonization with stem cells or osteoblasts 71,72 …”