Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers

Abstract

We investigated the proliferation and osteogenic differentiation of mesenchymal stem cells cultured on fibroin microcarriers. Effective cell proliferation on the surface of the microcarriers, determined by the large surface area, and the contribution of microcarrier mineralization to the stimulation of the osteogenic differentiation of mesenchymal stem cells was revealed.

Silk‐based microcarriers: current developments and future perspectives

“…Osteosarcoma cell lines (MG‐63) and MSCs cell adhesion occurred for all types of MCs. Cell proliferation was found to be significatively lower for mineralised MCs, probably since substrate mineralisation contributed to the induction of osteogenic differentiation in the absence of inductors, reducing the rate of proliferation [81]. While gelatin can be added to the material in order to promote the cellular response [76], calcium phosphate surface increases the MCs roughness.…”

“…Fibroin MCs modified by calcium phosphate mineralisation have also been studied. In the work of Kotliarova et al [29] and Goncharenko et al [81], porous MCs with the size ranging from 100 to 250 μm were obtained by cryodestruction of the matrices to generate fibroin, fibroin/gelatin, fibroin/calcium phosphate and fibroin/gelatin/calcium phosphate materials. Osteosarcoma cell lines (MG‐63) and MSCs cell adhesion occurred for all types of MCs.…”

Fabrication and characterization of osteogenic function of progenitor cell‐laden gelatin microcarriers

“…Particle size and size distribution can be modulated to some degree by varying the viscosity of the continuous phase, the rate of emulsification, and the impeller geometry, as well as by the addition of surfactants and post‐production filtration 23,24 . In contrast, microfluidic or flow‐focusing techniques are capable of generating particles with a narrow size distribution; however, the rate of production is comparatively low 25,26 …”

“…23,24 In contrast, microfluidic or flow-focusing techniques are capable of generating particles with a narrow size distribution; however, the rate of production is comparatively low. 25,26 Gelatin and other collagen-derived materials have been used extensively as base materials for microcarriers due to their broad availability, ease of processing, and generally excellent cytocompatibility.…”

“…The fabrication of collagen 23,30 and gelatin [31][32][33][34][35][36] microparticles has been demonstrated using bulk emulsification, 37,38 microfluidics, 39 coacervation, 40 and solgel synthesis. 12 In some cases these microcarriers are augmented with other macromolecules such as chitosan 41 or silk fibroin, 25,39 or with a mineral phase such as tricalcium phosphate, 15,30 calcium-deficient hydroxyapatite, 42,43 or hydroxyapatite. 38,44 Collagen and collagen-peptides foster the attachment and reorganization by many cell types, including a variety of progenitor cells known to be involved in bone formation.…”