Osteogenic Differentiation of Mouse Embryonic Stem Cells and Mouse Embryonic Fibroblasts in a Three-Dimensional Self-Assembling Peptide Scaffold

Garreta, Elena; Genové, Elsa; Borrós, Salvador; Semino, Carlos E.

doi:10.1089/ten.2006.12.2215

Cited by 142 publications

(68 citation statements)

References 51 publications

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“…the P19 embryonal carcinoma and the NIH/3T3 embryonic fibroblast cell lines. In contrast to normal embryonic stem cells, NIH/3T3 cells do not need specific growth factors (like the leukemia inhibiting factor) for selfrenewal [18][19][20][21] and therefore can be grown in the same culture media as do the P19 cells. Consequently, we used the same culture conditions for both cell models in order to avoid artefacts which are known to be linked to differences in cell culture content [16,17].…”

Section: Discussionmentioning

confidence: 99%

“…This limited pluripotent cell line was originally established from primary mouse embryonic fibroblasts and was spontaneously immortalized with stable growth rate, without oncogenic transformation. In contrast to normal embryonic stem (ES) cells, NIH/3T3 cell line does not need restrictive culture conditions and can easily be transfected for studying cell commitment [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Selective proapoptotic activity of polyphenols from red wine on teratocarcinoma cell, a model of cancer stem-like cell

et al. 2009

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Cancer stem cells are expected to be responsible for tumor initiation and metastasis. These cells are therefore potential targets for innovative anticancer therapies. However, the absence of bona fide cancer stem cell lines is a real problem for the development of such approaches. Since teratocarcinoma cells are totipotent stem cells with a high degree of malignancy, we used them as a model of cancer stem cells in order to evaluate the anticancer chemopreventive activity of red wine polyphenols (RWPs) and to determine the underlying cellular and molecular mechanisms. We therefore investigated the effects of RWPs on the embryonal carcinoma (EC) cell line P19 which was grown in the same culture conditions as the most appropriate normal cell line counterpart, the pluripotent embryonic fibroblast cell line NIH/3T3. The present study indicates that RWPs selectively inhibited the proliferation of P19 EC cells and induced G1 cell cycle arrest in a dose-dependent manner. Moreover, RWPs treatment specifically triggered apoptosis of P19 EC cells in association with a dramatic upregulation of the tumor suppressor gene p53 and caspase-3 activation. Our findings suggest that the chemopreventive activity of RWPs on tumor initiation and development is related to a growth inhibition and a p53-dependent induction of apoptosis in teratocarcinoma cells. In addition, this study also shows that the EC cell line is a convenient source for studying the responses of cancer stem cells to new potential anticancer agents.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selective proapoptotic activity of polyphenols from red wine on teratocarcinoma cell, a model of cancer stem-like cell

et al. 2009

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“…Mesenchymal progenitors were isolated from e18.5 embryos as previously described [23, 24] to allow comparison of differentiation capacity of Col3 wild-type and null cells. Cells were expanded in culture to generate sufficient numbers.…”

Section: Methodsmentioning

confidence: 99%

Type III Collagen Regulates Osteoblastogenesis and the Quantity of Trabecular Bone

et al. 2014

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Type III Collagen (Col3), a fibril-forming collagen, is a major extracellular matrix component in a variety of internal organs and skin. It is also expressed at high levels during embryonic skeletal development and is expressed by osteoblasts in mature bone. Loss of function mutations in the gene encoding Col3 (Col3a1) are associated with vascular Ehlers Danlos Syndrome (EDS). Although the most significant clinical consequences of this syndrome are associated with catastrophic failure and impaired healing of soft tissue structures, several studies have documented skeletal abnormalities in vascular EDS patients. However, there are no reports of the role of Col3 deficiency on the murine skeleton. We compared craniofacial and skeletal phenotypes in young (6-8 weeks) and middle-aged (>1 year) control (Col3+/+) and haploinsufficient (Col3+/−) mice, as well as young null (Col3−/−) mice using microcomputed tomography (μCT). Although Col3+/− mice did not have significant craniofacial abnormalities based upon cranial morphometrics, microCT analysis of distal femur trabecular bone demonstrated significant reductions in bone volume (BV), bone fraction volume (BV/TV), connectivity density (ConnD), structure model index (SMI) and trabecular thickness (TbTh) in young adult, female Col3+/− mice relative to wild-type littermates. The reduction in BV/TV persisted in female mice at one year of age. Next we evaluated the role of Col3 in vitro. Osteogenesis assays revealed that cultures of mesenchymal progenitors harvested from Col3−/− embryos display decreased alkaline phosphatase activity and reduced capacity to undergo mineralization. Consistent with this data, a reduction in osteogenic markers (type I collagen, osteocalcin and bone sialoprotein) correlates with reduced bone Col3 expression in Col+/− mice and with age in vivo. A small but significant reduction in osteoclast numbers was found in Col3+/− compared to Col3+/+ bones. Taken together, these findings indicate that Col3 plays a role in development of trabecular bone through its effects on osteoblast differentiation.

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“…141 Besides that, mouse embryonic stem cells and mouse embryonic fibroblasts have shown to differentiate into osteoblast-like cells possibly caused by the unique 3D-microenvironment originated from these nanofiber hydrogels. 142 Furthermore, the RADA16 hydrogels have been injected in the myocardium which led to the recruitment of progenitor cells that expressed endothelial markers. 143 Also vascular smooth muscle cells were recruited to the injected microenvironment, showing the formation of functional vascular structures.…”

Section: Supramolecular Biomaterialsmentioning

confidence: 99%

Supramolecular Biomaterials. A Modular Approach towards Tissue Engineering

Dankers

Meijer²

2007

Bulletin of the Chemical Society of Japan

123

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Supramolecular chemistry is an exciting area of science that plays a central role in bringing different disciplines together, ranging from molecular medicine to nanotechnology. Materials science based on supramolecular interactions is an emerging field, which has made important steps forward in the past ten years. The self-assembly of small synthetic molecules into long-chain architectures gives rise to the careful design of supramolecular polymers or fibers based on highly directional, reversible, non-covalent interactions. Much afford is put into the development of supramolecular (polymeric) materials with true materials properties, both in solution and in the solid state. These supramolecular materials are beginning to reach the market in all kind of applications. The field of regenerative medicine in general and that of tissue engineering in particular is one of the most challenging areas in which supramolecular materials might have a high potential. In tissue engineering, the biological environment and the interactions of cells with the artificial biomaterial is of utmost importance for the functioning of the implant, i.e. the engineered tissue. Ideal biomaterials do not only have to fulfil the biomaterials trinity of tuneable mechanical properties, regulation of the degradability and the ease for bioactivity incorporation, but also have to mimic the natural environment where the materials are brought into. Therefore, a modular, self-assembly approach using several supramolecular building blocks is an exquisite way to produce such ''responsive'' biomaterials. It is proposed that the artificial materials described in this account have the same type of dynamic ability to adapt its biofunctionality as is so well known for the living cells in the host tissue. This account will highlight two systems, i.e. self-assembling oligopeptide fibers as pioneered by Stupp et al. and Zhang et al., and our hydrogen-bonded supramolecular polymers, to show the potential of a modular approach to dynamic biomaterials for tissue engineering.

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Osteogenic Differentiation of Mouse Embryonic Stem Cells and Mouse Embryonic Fibroblasts in a Three-Dimensional Self-Assembling Peptide Scaffold

Cited by 142 publications

References 51 publications

Selective proapoptotic activity of polyphenols from red wine on teratocarcinoma cell, a model of cancer stem-like cell

Selective proapoptotic activity of polyphenols from red wine on teratocarcinoma cell, a model of cancer stem-like cell

Type III Collagen Regulates Osteoblastogenesis and the Quantity of Trabecular Bone

Supramolecular Biomaterials. A Modular Approach towards Tissue Engineering

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