Osteogenic effect of local, long versus short term BMP-2 delivery from a novel SPU–PLGA–βTCP concentric system in a critical size defect in rats

Rodríguez-Évora, María; Delgado, Araceli; Reyes, Ricardo; Hernández-Daranas, A.; Soriano, I.; Román, Julio San; Évora, Carmen

doi:10.1016/j.ejps.2013.06.008

Cited by 46 publications

(52 citation statements)

References 31 publications

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“…Cowan et al studied the regeneration potential of the MSCs cultured on the polylactide/hydroxyapatite scaffolds and implanted to the mouse cranial defect; at 12 weeks, the defect was completely regenerated. Simvastatin loaded gelatin‐nanofibrillar cellulose‐β tricalcium phosphate (GNTS) hydrogel scaffolds and systems composed of segmented polyurethane, poly[lactic‐co‐glycolic acid], and β‐tricalcium phosphate in conjunction with rhBMP‐2 implanted into 8‐mm rat cranial defects generated only 60% bone tissue over 12 weeks . Seung‐Hwan Jegal used composite nano‐scaffolds based on polylactide and polycaprolactone carrying gelatin and apatite to close 5‐mm rat cranial defects; in spite of the poor adhesive properties and low mechanical strength of the scaffolds, bone formation occurred both in the center and around the edges of the defects; at 6 weeks, the defect was 85% regenerated.…”

Section: Discussionmentioning

confidence: 99%

Porous 3D implants of degradable poly‐3‐hydroxybutyrate used to enhance regeneration of rat cranial defect

Shumilova

Мылтыгашев

Кириченко

et al. 2016

J Biomedical Materials Res

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The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid P(3HB) for regeneration of bone tissue defects. The ability of the P(3HB) implants to favor attachment and facilitate proliferation and directed differentiation of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. Tissue-engineered hybrid systems (grafts) constructed using P(3HB) and P(3HB) in combination with osteoblasts were used in experiments on laboratory animals (n = 48) with bone defect model. The defect model (5 mm in diameter) was created in the rat parietal bone, and filling of the defect by the new bone tissue was monitored in the groups of animals with P(3HB) implants, with commercial material, and without implants (negative control). Computed tomography (CT) and histologic examination showed that after 120 days, in the group with the osteoblast-seeded P(3HB) implants, the defect was completely closed; in the group with the cell-free P(3HB) implants, the remaining defect was no more than 10% of the initial one (0.5 mm); in both the negative and positive controls, the size of the defect was about 1.0-1.2 mm. These results suggest that P(3HB) has good potential as osteoplastic material for reconstructive osteogenesis. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 566-577, 2017.

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Section: Discussionmentioning

confidence: 99%

Porous 3D implants of degradable poly‐3‐hydroxybutyrate used to enhance regeneration of rat cranial defect

Shumilova

Мылтыгашев

Кириченко

et al. 2016

J Biomedical Materials Res

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“…3). These results demonstrate that controlling the presence of rh-BMP-2 is necessary to induce bone regeneration at the defect site [89]. …”

Section: Nanotherapeutics For Healing Bone After Traumamentioning

confidence: 96%

“…Although cells and tissues penetrated throughout the scaffolds onto which rhBMP-7 had been adsorbed as well as those treated with rhBMP-7 NPs, histologic analysis revealed that the incorporation of rhBMP-7 into the NPs protected the protein's bioactivity, prolonged delivery, and subsequently induced ectopic cone formation; in contrast, simple adsorption of rhBMP-7 onto the scaffolds failed to stimulate bone formation [87]. Rodriguez-Evora et al investigated the bone-inducing efficacy of a composite composed of a porous ring of tribasic calcium phosphate (β-TCP) filled with a pasty core of segmented polyurethane (SPU) and PLGA microspheres (212 μm) encapsulating rhBMP-2 [89]. In vivo experiments showed that 65% of rhBMP-2 was cleared from the scaffolds containing non-loaded PLGA microspheres and rhBMP-2 in solution during the first day, in comparison with 25% of the protein from the scaffolds containing rhBMP-2-loaded PLGA microspheres.…”

Section: Nanotherapeutics For Healing Bone After Traumamentioning

confidence: 99%

Nanomedicine for safe healing of bone trauma: Opportunities and challenges

et al. 2017

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Historically, high-energy extremity injuries resulting in significant soft-tissue trauma and bone loss were often deemed unsalvageable and treated with primary amputation. With improved soft-tissue coverage and nerve repair techniques, these injuries now present new challenges in limb-salvage surgery. High-energy extremity trauma is pre-disposed to delayed or unpredictable bony healing and high rates of infection, depending on the integrity of the soft-tissue envelope. Furthermore, orthopedic trauma surgeons are often faced with the challenge of stabilizing and repairing large bony defects while promoting an optimal environment to prevent infection and aid bony healing. During the last decade, nanomedicine has demonstrated substantial potential in addressing the two major issues intrinsic to orthopedic traumas (i.e., high infection risk and low bony reconstruction) through combatting bacterial infection and accelerating/increasing the effectiveness of the bone-healing process. This review presents an overview and discusses recent challenges and opportunities to address major orthopedic trauma through nanomedical approaches.

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“…25,41,42 Similarly, in vitro studies using poly( l -lysine) (PLL) nanoparticles to deliver BMP-2 within a fibrin hydrogel showed enhanced osteogenic differentiation of bone marrow-derived mesenchymal cells 43 In vivo studies using this strategy have also revealed BMP-2-coated PLGA nanoparticles within a fibrin hydrogel complex to be capable of significantly enhancing bone regeneration in a critical-sized rat calvarial defect. 44,45 And like BMP-2, BMP-7 has been encapsulated in PLGA nanospheres, resulting in temporally controlled release and ectopic bone formation following subcutaneous implantation on nano-fibrous PLA scaffolds in rats. 46 These findings thus underscore the applicability of nanoparticles for delivery of growth factors in novel bone regenerative strategies.…”

Section: Nanoparticle-based Deliverymentioning

confidence: 99%

Nanotechnology in bone tissue engineering

Walmsley

McArdle

Tevlin

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

236

162

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Nanotechnology represents a major frontier with potential to significantly advance the field of bone tissue engineering. Current limitations in regenerative strategies include impaired cellular proliferation and differentiation, insufficient mechanical strength of scaffolds, and inadequate production of extrinsic factors necessary for efficient osteogenesis. Here we review several major areas of research in nanotechnology with potential implications in bone regeneration: 1) nanoparticle-based methods for delivery of bioactive molecules, growth factors, and genetic material, 2) nanoparticle-mediated cell labeling and targeting, and 3) nano-based scaffold construction and modification to enhance physicochemical interactions, biocompatibility, mechanical stability, and cellular attachment/survival. As these technologies continue to evolve, ultimate translation to the clinical environment may allow for improved therapeutic outcomes in patients with large bone deficits and osteodegenerative diseases.

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Osteogenic effect of local, long versus short term BMP-2 delivery from a novel SPU–PLGA–βTCP concentric system in a critical size defect in rats

Cited by 46 publications

References 31 publications

Porous 3D implants of degradable poly‐3‐hydroxybutyrate used to enhance regeneration of rat cranial defect

Porous 3D implants of degradable poly‐3‐hydroxybutyrate used to enhance regeneration of rat cranial defect

Nanomedicine for safe healing of bone trauma: Opportunities and challenges

Nanotechnology in bone tissue engineering

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