Osteogenic growth peptide modulates fracture callus structural and mechanical properties

Gabet, Yankel; Müller, Ralph; Regev, Eran; Sela, J.; Shteyer, A.; Salisbury, Kristy; Chorev, Michael; Bab, Itai

doi:10.1016/j.bone.2004.03.025

Cited by 101 publications

(71 citation statements)

References 39 publications

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“…A bifunctional Becker and co-workers later exploited their CuAAC technology to couple osteogenic growth peptide (OGP) to SAMs in order to explore the effects of OGP density on pre-osteoblast cell adhesion, morphology, and proliferation 112 . OGP has been recognized as a promising agent for bone tissue engineering applications because it stimulates tissue regeneration of bone defects 113 .…”

Section: Cuaac Click Immobilizationmentioning

confidence: 99%

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

2011

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Hyaluronic acid (HA) is a naturally occurring polymer that holds considerable promise for tissue engineering applications. Current cross-linking chemistries often require a coupling agent, catalyst, or photoinitiator, which may be cytotoxic, or involve a multistep synthesis of functionalized-HA, increasing the complexity of the system. With the goal of designing a simpler one-step , aqueous-based cross-linking system, we synthesized HA hydrogels via Diels-Alder "click" chemistry. Furan-modified HA derivates were synthesized and cross-linked via dimaleimide poly(ethylene glycol). By controlling the furan to maleimide molar ratio, both the mechanical and degradation properties of the resulting Diels-Alder cross-linked hydrogels can be tuned. Rheological and degradation studies demonstrate that the Diels-Alder click reaction is a suitable cross-linking method for HA. These HA cross-linked hydrogels were shown to be cytocompatible and may represent a promising material for soft tissue engineering.iii

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Section: Cuaac Click Immobilizationmentioning

confidence: 99%

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

2011

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“…This fragment directly interacts with cell membrane receptors, activating the MAP kinase, Src and RhoA signaling pathways [9][10][11]. Upon intravenous administration, synthetic OGP and OGP10-14 were shown to promote increased bone mass and fracture healing in vivo [12,13]. In vitro, OGP peptides were shown to increase the proliferation of osteoblastic-like cells and MSC and accelerate osteogenesis [14,15].…”

Section: Introductionmentioning

confidence: 99%

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Maia

Barbosa²,

Gomes

et al. 2014

Journal of Controlled Release

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The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration.

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“…To differentiate newly formed mineralized callus from old cortices, the low-and high-density mineralized tissues were reconstructed using different thresholds (high attenuation ¼ 325, low attenuation ¼ 120) defined in 2D images using our recently established evaluation protocol. 42,43 The high-density tissues represented old cortices and newly formed, highly mineralized callus, whereas the low-density tissues represented newly formed callus. 42,43 Quantitative analysis was performed covering the mid 300 slices of the 2D images with the low-and high-density mineralized tissues evaluated separately.…”

Section: Micro-ct Analysismentioning

confidence: 99%

“…42,43 The high-density tissues represented old cortices and newly formed, highly mineralized callus, whereas the low-density tissues represented newly formed callus. 42,43 Quantitative analysis was performed covering the mid 300 slices of the 2D images with the low-and high-density mineralized tissues evaluated separately. Morphometric parameters used for evaluation were total tissue volume (TV, mm 3 , calculated from the contoured ROI in 2D images), volume of high-density bone (BV h , mm 3 ), volume of low-density bone (BV l , mm 3 ), total bone volume (BV t , mm 3 , i.e., equivalent to BV h þ BV l , or TV-interstitial space) and normalized percentage of the tissue volumes including BV h / TV, BV l /TV, BV t /TV.…”

Section: Micro-ct Analysismentioning

confidence: 99%

Low‐magnitude high‐frequency vibration accelerates callus formation, mineralization, and fracture healing in rats

Leung

Shi

Cheung

et al. 2009

Journal Orthopaedic Research

104

130

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Fracture healing is a biological regenerative process that follows a well-orchestrated sequence. Most healing is uneventful and enhancement of normal fracture healing is not commonly done, although it is clinically important in the recovery and regain of functions after fracture. This study investigated the osteogenic effect of low-magnitude high-frequency vibration (LMHFV, 35 Hz, 0.3 g) on the enhancement of fracture healing in rats with closed femoral shaft fracture by comparing with sham-treated control. Assessments with plain radiography, micro-CT as well as histomorphometry showed that the amount of callus was significantly larger (p ¼ 0.001 for callus area, 2 weeks posttreatment); the remodeling of the callus into mature bone was significantly faster ( p ¼ 0.039, 4 weeks posttreatment) in the treatment group. The mechanical strength of the healed fracture in the treatment group at 4 weeks was significantly greater ( p < 0.001). The results showed the acceleration of callus formation, mineralization, and fracture healing in the treatment group. It is concluded that LMHFV enhances healing in the closed femoral shaft fracture in rats. The potential clinical advantages shall be confirmed in the subsequent clinical trials. ß

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Osteogenic growth peptide modulates fracture callus structural and mechanical properties

Cited by 101 publications

References 39 publications

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Low‐magnitude high‐frequency vibration accelerates callus formation, mineralization, and fracture healing in rats

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