Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Samiei, Mohammad; Aghazadeh, Marziyeh; Aminabadi, Naser Asl; Davaran, Soodabeh; Shirazi, Sajjad; Ashrafi, Farhad; Salehi, Roya

doi:10.15171/apb.2016.047

Cited by 34 publications

(39 citation statements)

References 45 publications

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“…In the oral and maxillofacial regions for the regeneration of bone defects, the non-invasive injectable methods for stem cell delivery are more useful (62). The osteo/odontogenic differentiation of hDPSCs on various scaffolds was evaluated previously (41,43,63,64). However, none of these scaffolds evaluated non-invasive injectable carriers for these cells.…”

Section: Discussionmentioning

confidence: 99%

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

Alipour

Firouzi

Aghazadeh

et al. 2020

Preprint

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Background Microcapsule is considered as a promising 3D microenvironment for Bone Tissue Engineering (BTE) applications. Microencapsulation of cells in an appropriate scaffold not only protects cells against excess stress but also promotes cell proliferation and differentiation. Methods In the current study, human Dental Pulp Stem Cells (hDPSCs) were cultivated in Alginate/Gelatin (Alg/Gel) and Alginate/Gelatin/nano-Hydroxyapatite (Alg/Gel/nHA) microcapsules. The proliferation and osteogenic differentiation of these cells were evaluated by MTT assay, qRT-PCR, Alkaline phosphatase, and Alizarine Red S.Results The results revealed that microencapsulation by Alg/Gel/nHA could improve cell proliferation and induce osteogenic differentiation. The cells cultured in the Alg/Gel and Alg/Gel/nHA microcapsules after 28 days showed 2.5-fold and 4-fold more activity of BMP-2 gene expression in comparison with the cells cultured on the polystyrene surface as a control group. The nHA addition to hDPSCs-laden Alg/Gel microcapsule could also up-regulate the bone-related gene expressions of osteocalcin, osteonectin, and RUNX-2 during a 28-day culture period. Calcium deposition and ALP activities of the cells were obsereved in accordance with the proliferation results as well as the gene expression analysis. Conclusion The study demonstrated that microencapsulation of hDPSCs inside Alg/Gel/nHA hydrogel can be a potential approach for regenerative dentistry in the near future.

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Section: Discussionmentioning

confidence: 99%

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

Alipour

Firouzi

Aghazadeh

et al. 2020

Preprint

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“…4,19 Various scaffolds have been utilized for simvastatin use. 15,[21][22][23][24] A hydrogel composed of gelatin-poly(ethylene glycol)-tyramine has been used as an efficient simvastatin delivery vehicle to trigger osteogenic differentiation. 21 Calcium phosphate composite scaffolds containing simvastatin-loaded poly(lactic-co-glycolic acid) microspheres have been fabricated, and MSCs have been seeded onto the scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…23 It has also been incorporated into hydroxyapatite in poly(ε-caprolactone)/polylactic acid polymeric scaffolds for osteogenic bioengineering. 24 Simvastatin-stimulated osteogenesis is mediated by estrogen receptor α. 16 It has also been noted that simvastatin enhances the Rho/actin/cell rigidity pathway, contributing to osteogenic differentiation of MSCs; both keeping the actin cytoskeletons intact and enhancing cell rigidity is crucial in simvastatin-induced osteogenesis.…”

Section: Discussionmentioning

confidence: 99%

The effects of simvastatin on cellular viability, stemness and osteogenic differentiation using 3-dimensional cultures of stem cells and osteoblast-like cells

Lee

Park

2019

Adv Clin Exp Med

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Background. Simvastatin has been reported to increase the therapeutic effects of many kinds of stem cells by increasing the number of those cells. However, the effects of simvastatin on the differentiation potential of stem cells have not been clearly determined. Objectives. The aim of the study was to evaluate the effects simvastatin has on cellular viability, stemness and osteogenic differentiation using 3-dimensional cell spheroids of stem cells and osteoblast-like cells. Material and methods. Three-dimensional cell spheroids were fabricated using concave silicon elastomerbased microwells in the presence of simvastatin at concentrations of 1 μM and 10 μM. Qualitative cellular viability was determined with a confocal microscope, and quantitative cellular viability was evaluated using a cell-counting assay kit. The expression of stem cell surface markers was tested. A quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate the expression of collagen I and RUNx2. Alkaline phosphatase activity and alizarin red S staining were used to assess osteogenic differentiation. Results. The spheroids formed well in the concave silicon elastomer-based microwells, and the application of simvastatin caused no significant morphological changes. No significant changes in cellular viability were noted with the addition of simvastatin on days 1, 3 and 5. Secretion of the vascular endothelial growth factor (VEGF) was observed on day 1 and remained stable throughout the culture period. Expression of the CD90 surface marker was seen on day 7. The addition of simvastatin caused a statistically significant increase in the expression of collagen I and RUNX2. It also caused decreases in alkaline phosphatase activity and alizarin red S staining. Conclusions. The study clearly showed that the application of simvastatin enhanced collagen I and RUNX2 expression; however, this did not lead to increases in alkaline phosphatase activity or alizarin red S staining.

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“…Afterward, the cells were subcultured or seeded on the prepared scaffolds at a seeding density of 20,000–30,000 cells per scaffold. Simvastatin powder was dissolved in ethanol at a concentration of 1 μmol L −1 and added to the cell cultures . Two wells were selected as the controls without simvastatin addition.…”

Section: Methodsmentioning

confidence: 99%

In situsynthesized chitosan–gelatin/ZnO nanocomposite scaffold with drug delivery properties: Higher antibacterial and lower cytotoxicity effects

Rakhshaei

Namazi

Hamishehkar

et al. 2019

J of Applied Polymer Sci

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107

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In this work, chitosan–gelatin/zinc oxide nanocomposite hydrogel scaffolds (CS–GEL/nZnO) were prepared via in situ synthesis of ZnO nanoparticles (nZnO) to reach a scaffold with both inherent antibacterial and drug delivery properties. The prepared nanocomposite hydrogel scaffolds were characterized using scanning electron microscopy, transmission electron microscopy, atomic absorption spectrometer, Fourier transform infrared spectroscopy, and X‐ray diffraction. In addition, swelling, biodegradation, antibacterial, cytocompatibility, and cell attachment of the scaffolds were evaluated. The results showed that the prepared scaffolds had high porosity with a pore size of 50–400 μm and nZnO were well distributed without any agglomeration on the CS–GEL matrix. In addition, the nanocomposite scaffolds showed enhanced swelling, biodegradation, and antibacterial properties. Moreover, the drug delivery studies using naproxen showed that nZnO could control naproxen release. Cytocompatibility of the samples was proved using normal human dermal fibroblast cells (HFF2). In comparison to the previous reports which nZnO were simply added to the matrix of the scaffold, in situ synthesis of nZnO was led to higher antibacterial and lower cytotoxicity effects as a result of well distribution of nZnO in this method. According to the findings, the in situ synthesized CS–GEL/nZnO is strongly recommended for biomedical applications especially skin tissue engineering. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47590.

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Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Cited by 34 publications

References 45 publications

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

The effects of simvastatin on cellular viability, stemness and osteogenic differentiation using 3-dimensional cultures of stem cells and osteoblast-like cells

In situsynthesized chitosan–gelatin/ZnO nanocomposite scaffold with drug delivery properties: Higher antibacterial and lower cytotoxicity effects

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