Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells

Park, Bong-Wook; Hah, Young‐Sool; Kim, Deok Ryong; Kim, Jongryoul; Byun, June‐Ho

doi:10.1016/j.archoralbio.2007.04.007

Cited by 70 publications

(84 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the decrease in Sox9 expression observed in osteogenic stimulated cells indicates that the cells are not entering the chondrogenic lineage [24,25]. This is confirmed by Runx2 expression, as Runx2 is known to promote differentiation into the mature osteoblast and then further into the osteocyte termination [26,27]. The expression of Runx2 is enhanced in both FGFR3 mutants cultured in conditions promoting osteogenesis, compared to unstimulated cells.…”

Section: Discussionsupporting

confidence: 57%

See 1 more Smart Citation

The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Differentiation and Ectopic Bone Formation by Human Periosteal Derived Cells

Bolander¹,

Roberts²,

Eyckmans³

et al. 2012

J Tissue Sci Eng

View full text Add to dashboard Cite

Activating mutations in Fibroblast Growth Factor Receptor 3 (FGFR3) have previously been shown to cause skeletal dysplasias through their effect on growth plate chondrocytes. However, the effect of FGFR3 mutations on bone progenitor cells may differ. The objective of this study was to investigate the effect of specific activating FGFR3 mutations on ectopic in vivo bone formation by periosteal derived cells (PDCs) seeded on calcium phosphate/ collagen scaffolds.PDCs were isolated from hypochondroplasic (N540K mutation) and achondroplasic (G380R mutation) patients, along with age/sex matched controls. These cells were characterised in vitro for proliferation, osteogenic differentiation, FGFR3 signalling and in vivo bone formation. Subsequently, empirical modelling was used to find correlations between in vivo formed bone and in vitro cell behaviour. These data showed that in contrast to the G380R mutation, which produced no bone, the N540K mutation induced significant ectopic bone formation on specific carriers. This allowed correlation between percentage of induced bone formation to elevated in vitro proliferation and differentiation. Correlating osteogenic markers included Collagen type 1, alkaline phosphatase and osteocalcin. Enhanced proliferation was attributed to increased phosphorylation of Erk-1/2. This study highlights the importance of FGFR3 in periosteal cell differentiation and also indicates it potential for targeted tissue engineering strategies.

show abstract

Section: Discussionsupporting

confidence: 57%

“…This hypothesis is strengthened by the increased expression of additional osteogenic markers such as ALP, Coll1 and osteocalcin in N540K cells. Together, these represent early to fully differentiated osteoblasts [27,28].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Differentiation and Ectopic Bone Formation by Human Periosteal Derived Cells

Bolander¹,

Roberts²,

Eyckmans³

et al. 2012

J Tissue Sci Eng

View full text Add to dashboard Cite

show abstract

“…We speculate two possible explanations for this inconsistency. Like trabecular osteoblasts, periosteal osteoprogenitor cells are considered to have high levels of ALP activity in young growing rats (Mizuno et al 2006;Park et al 2007). Thus, it is possible that periosteal osteoprogenitor cells rather than trabecular osteoblasts were affected more strongly by the long duration exercise-induced decrease in bone formation, resulting in significant loss of BMD of the cortex.…”

Section: Discussionmentioning

confidence: 99%

Running Exercise for Short Duration Increases Bone Mineral Density of Loaded Long Bones in Young Growing Rats

Hagihara

Nakajima

Fukuda³

et al. 2009

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

“…19 These progenitor (stem) cells have been shown to differentiate into osteocytes, adipocytes and chondrocytes. [20][21][22][23] We have earlier isolated dental follicle cells and found that they can be efficiently transfected with non-viral methods, suggesting a potential for future gene-based cell therapies. 24 Similarly, the dental pulp cells also differentiate into several cell types, such as osteocytes, neurons, adipocytes and chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

et al. 2009

View full text Add to dashboard Cite

A number of studies have reported in the last decade that human tooth germs contain multipotent cells that give rise to dental and peri-odontal structures. The dental pulp, third molars in particular, have been shown to be a significant stem cell source. In this study, we isolated and characterized human tooth germ stem cells (hTGSCs) from third molars and assessed the expression of developmentally important transcription factors, such as oct4, sox2, klf4, nanog and c-myc, to determine their pluri-potency. Flowcytometry analysis revealed that hTGSCs were positive for CD73, CD90, CD105 and CD166, but negative for CD34, CD45 and CD133, suggesting that these cells are mesenchymal-like stem cells. Under specific culture conditions, hTGSCs differentiated into osteogenic, adipogenic and neurogenic cells, as well as formed tube-like structures in Matrigel assay. hTGSCs showed significant levels of expression of sox2 and c-myc messenger RNA (mRNA), and a very high level of expression of klf4 mRNA when compared with human embryonic stem cells. This study reports for the first time that hTGSCs express developmentally important transcription factors that could render hTGSCs an attractive candidate for future somatic cell re-programming studies to differentiate germs into various tissue types, such as neurons and vascular structures. In addition, these multipotential hTGSCs could be important stem cell sources for autologous transplantation.

show abstract

Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells

Cited by 70 publications

References 36 publications

The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Differentiation and Ectopic Bone Formation by Human Periosteal Derived Cells

The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Differentiation and Ectopic Bone Formation by Human Periosteal Derived Cells

Running Exercise for Short Duration Increases Bone Mineral Density of Loaded Long Bones in Young Growing Rats

Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

Contact Info

Product

Resources

About