Osteoinductivity of Partially Decalcified Alloimplants in Healing of Large Osteoperiosteal Defects

Gupta, D. K.; Tuli, SM

doi:10.3109/17453678208992839

Cited by 28 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The decalcified bone graft has shown to be an osteoinductive and osteoconductive material under various experimental conditions. The insignificant immunological reaction refutes the concerns about the antigenicity 102627. Winkler et al 28.…”

Section: Discussionmentioning

confidence: 59%

Decal bone matrix as a local antibiotic delivery vehicle in a MRSA-infected bone model: An experimental study

Saraf

Yadav

Nagwani

et al. 2010

IJOO

View full text Add to dashboard Cite

Background:Polymethyl methacrylate (PMMA) antibiotic beads though have proved their utility as a local antibiotic delivery system, however, there are limitations. Decalcified bone matrix (DBM) as a vehicle of antibiotics can serve the purpose, provided a minimum inhibitory concentration is sustained. Healing of the defect and avoiding the second surgery is another advantage. We studied the DBM as the delivery vehicle for vancomycin in controlling the methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis as well as healing of the cavity simultaneously in an experimental study.Materials and Methods:An in vitro study was conducted to optimize vancomycin impregnation in the DBM. For the in vivo study, a unicortical defect was created in the metaphysis of the distal femur in 18 rabbits. After contaminating the defect with MRSA, rabbits were divided into three groups. Group I (eight limbs) received no graft. Defects in group II (11 limbs) were filled with plain DBM chips and in group III (14 limbs), cavities were implanted with vancomycin-impregnated decal bone chips. Rabbits were assessed by clinical, radiological, histological, gross examination and bacterial load assay. High Performance Liquid Chromatography HPLC analysis of vancomycin in group III was done to assess the concentration in DBM chips.Results:In group I, the infection persisted throughout the period of the study. Group II showed the fulminated infection at the grafted site with DBM chips sequestrating out. Vancomycin-impregnated decal chips in group III did not show any sign of infection and eventually incorporated. The bacterial load study showed a progressive load change and HPLC revealed an effective antibiotic concentration up to 3 weeks in both in vitro and in vivo.Conclusion:Decal bone chips were effective as the local antibiotic delivery vehicle in preventing the MRSA osteomyelitis model. It eluted vancomycin significantly and the graft uptake was also excellent. Allogeneic decal grafts eliminated the need for second surgery and acted as an excellent delivery vehicle for antibiotics.

show abstract

Section: Discussionmentioning

confidence: 59%

Decal bone matrix as a local antibiotic delivery vehicle in a MRSA-infected bone model: An experimental study

Saraf

Yadav

Nagwani

et al. 2010

IJOO

View full text Add to dashboard Cite

show abstract

“…9,11 An increase in osteogenic cells was seen on the 20th postoperative day in autografts (30-75%), while it was slightly delayed in allografts and was seen on the 30th postoperative day (45-80%). The osteogenic activity was comparable for controls, autografts and allografts on the 40th postoperative day.…”

Section: Garg Et Almentioning

confidence: 96%

“…In developing countries with a perennial power shortage, the partially decalcified allogenic bone (decalbone) has been shown to be a good substitute for autografts in various clinical and experimental studies. 6,7,9,10,12,[14][15][16][17][18][19][20] The current problems of graft relate less to technique and more to cellular events that occur during the early phase of graft uptake. Earlier experimental studies with bone grafts in animals cannot always be extrapolated to humans.…”

Section: Objective: To Conceptualize With Fine Needle Aspiration Cytmentioning

confidence: 99%

Early Biologic Behavior of Bone Grafts

Dev¹,

Misra²,

Tuli³

1997

Acta Cytologica

View full text Add to dashboard Cite

“…There have been numerous publications in which the authors have addressed the osteogenic capacity of various skeletal tissues and their combinations, or specially treated tissue derivatives (Burwell 1966 Lindholm et al 1982, Volpon et al 1982, Gupta & Tuli 1982, Cummine et al 1983, Wittbjer et al 1983. McDavid et al (1979) found that porous ceramic implants impregnated with bone mattow had bone formation within the pores.…”

Section: Discussionmentioning

confidence: 99%