Osteointegration of hydroxyapatite-titanium implants coated with nonglycosylated recombinant human bone morphogenetic protein-2 (BMP-2) in aged sheep

Sachse, A.; Wagner, Andreas; Keller, Mark R.; Wagner, Otto; Wetzel, Wolf D.; Layher, Frank; Venbrocks, R.; Hortschansky, Peter; Pietraszczyk, M.; Wiederanders, Bernd; Hempel, Hans-Jürgen; Bossert, Jörg; Horn, Joachim; Schmuck, K.; Mollenhauer, Jürgen

doi:10.1016/j.bone.2005.06.011

Cited by 102 publications

(91 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…39,40 It has been demonstrated that different implant surface microtopographies can alter the expression of key osteogenic regulatory genes in preosteoblast cells, suggesting that the interaction of the cells with extracellular matrix (ECM) components, the organization of the actin cytoskeleton as well as activities in signal transduction pathways are associated with implant surface microtopographies. 41,42 Perhaps this occurs as a result of differences in patterns of cell proliferation and differentiation as a result of integrin-mediated adhesion regulation of downstream signaling cascades and ECM spatial and temporal expression profile changes as previously reported.…”

Section: Discussionmentioning

confidence: 99%

The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification

Rausch‐Fan

Wieland

et al. 2007

J Biomedical Materials Res

115

View full text Add to dashboard Cite

The aim of this study was to investigate the influence of hydrophobic acid-etched (A) and coarse-blasted large-grit and acid-etched (SLA) surfaces as well as hydrophilic modified acid-etched (modA) and modified coarse-blasted large-grit and acid-etched (modSLA) surfaces on the behavior of MG63 cells grown on these surfaces through determination of cell attachment and cell proliferation, time-lapse microscopy of fluorescence-labeled cells, and determination of gene expression by reverse transcription-polymerase chain reaction (RT-PCR). No significant difference of cell attachment on various titanium surfaces was found. Increased cell proliferation was observed on the A surface and the SLA surface compared with the modA surface and the modSLA surface. After 2 days of incubation, on modSLA and modA surfaces a tendency of formation of cell clusters has been observed, which was most pronounced on modSLA surface. On the A and the SLA surface, cell cluster formation started after longer incubation periods. The expression level of the bone-associated genes (alkaline phosphatase, osteocalcin, type-I-collagen, osteoprotegerin, and glyceraldehyde-3-phosphate-dehydrogenase) detected by RT-PCR was highest on the modSLA surface. In conclusion it has been demonstrated that the modSLA surface results in an enhanced cluster formation of osteoblasts grown on this surface and in an increased expression of key osteogenic regulatory genes in osteoblasts.

show abstract

Section: Discussionmentioning

confidence: 99%

The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification

Rausch‐Fan

Wieland

et al. 2007

J Biomedical Materials Res

115

View full text Add to dashboard Cite

show abstract

“…The protein adsorption behavior also plays a vital role in the fields of biosensor design [3] and drug delivery systems [4]. So far a large number of studies have reported that proteins such as fibronectin, bone morphogenetic proteins and so forth can enhance cell adhesion to the implant surface or even promote osteoblastic differentiation and bone regeneration [5][6][7][8][9]. However, the protein adsorption characteristic of biomaterials and the mechanisms involved need further investigation.…”

Section: Introductionmentioning

confidence: 99%

Effect of phase composition and microstructure of calcium phosphate ceramic particles on protein adsorption

et al. 2010

View full text Add to dashboard Cite

“…In physiological settings, BMP-2 exerts its effects in nanogram doses [7], yet due to release from its site of application the recommended amounts applied to date are around 25 mg depending on the respective therapeutic regimen [5,6]. Taking this into account and in order to prolong the effect of freely-diffusible growth factors, slow releasing systems have been put to test [8][9][10]. This way of practice however still incurs the risk of adverse effects, which in particular may affect the vascular system [11] or the immune system [12].…”

Section: Introductionmentioning

confidence: 99%

The role of oxygen termination of nanocrystalline diamond on immobilisation of BMP-2 and subsequent bone formation

et al. 2008

View full text Add to dashboard Cite

Medical implants are increasingly often inserted into bone of frail patients, who are advanced in years. Due to age, severe trauma or pathology-related bone changes, osseous healing at the implant site is frequently limited. We were able to demonstrate that coating of endosseous implants with nanocrystalline diamond (NCD) allows stable functionalization by means of physisorption with BMP-2. Strong physisorption was shown to be directly related to the unique properties of NCD, and BMP-2 in its active form interacted strongest when NCD was oxygen-terminated. The binding of the protein was monitored under physiological conditions by single molecule force spectroscopy, and the respective adsorption energies were further substantiated by force-field-calculations. Implant surfaces refined in such a manner yielded enhanced osseointegration in vivo,when inserted into sheep calvaria. Our results further suggest that this technical advancement can be readily applied in clinical therapies with regard to bone healing, since primary human mesenchymal stromal cells strongly activated the expression of osteogenic markers when being cultivated on NCD physisorbed with physiological amounts of BMP-2.

show abstract

Osteointegration of hydroxyapatite-titanium implants coated with nonglycosylated recombinant human bone morphogenetic protein-2 (BMP-2) in aged sheep

Cited by 102 publications

References 26 publications

The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification

The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification

Effect of phase composition and microstructure of calcium phosphate ceramic particles on protein adsorption

The role of oxygen termination of nanocrystalline diamond on immobilisation of BMP-2 and subsequent bone formation

Contact Info

Product

Resources

About