Osteomyelitis Experimentally Induced With Bacteroides thetaiotaomicron and Staphylococcus epidermidis

Kj, Mayberry-Carson; Tober-Meyer, B; Dw, Lambe; Jw, Costerton

doi:10.1097/00003086-199207000-00040

Cited by 18 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The infection rate was comparable to other studies using Delivery of therapeutic agents in bone scaffolds rabbit models [14,15]. An advantage of using a rat model is that the size of specimens harvested that allows histological survey of the entire affected bone.…”

Section: Discussionsupporting

confidence: 67%

Potential of bone scaffolds containing vancomycin and bone morphogenetic protein-2 in a rat model of osteomyelitis

Thanyaphoo

Kaewsrichan

2014

Asian Biomedicine

View full text Add to dashboard Cite

Background: Infected bone is often intractable. An ideal approach is to simultaneously eradicate infection and repair the bone defect. The development of osteoinductive bone graft composites to control antibiotic drug release would be useful for the treatment of intractable bone infections. Objectives: To develop a rat model of osteomyelitis for assessing osteoinductive bone graft scaffolds containing antibiotics and a bone morphogenetic protein.Methods: Si-imprinted calcium phosphate is a new hydroxyapatite derivative used in fabricating bone scaffolds. Vancomycin and bone morphogenetic protein-2 (BMP-2) were loaded onto scaffolds of Si-imprinted calcium phosphate using an established method. The efficiency of the scaffold as a drug carrier system was assessed in vivo. Osteomyelitis was induced in rats by infection of the tibial epiphysis with Staphylococcus aureus (BAA 1680). The success of inducing disease was checked after 4 weeks using bacterial culture and radiography. A 10 mm metaphysis bone was surgically removed and replaced with a drug-loaded scaffold. Histology and X-ray imaging were used to evaluate the implants at 8 weeks post implantation. Results: We successfully established a rat model of osteomyelitis. The causative bacteria were effectively eradicated by vancomycin released from the implants. Enhanced bone formation was observed for the implant samples containing vancomycin and BMP-2 compared with those containing either vancomycin or BMP2 alone. Conclusions:The newly developed bone scaffold has potential as a vehicle for therapeutic agents to treat bone diseases.

show abstract

Section: Discussionsupporting

confidence: 67%

Potential of bone scaffolds containing vancomycin and bone morphogenetic protein-2 in a rat model of osteomyelitis

Thanyaphoo

Kaewsrichan

2014

Asian Biomedicine

View full text Add to dashboard Cite

show abstract

“…On the other hand, models using a precolonized implant mainly approach the situation of implanting contaminated material or use of a non-sterile procedure at surgery. Several of the local infection models developed so far have been based on use of a bacterial suspension together with implantation of sterile metal pieces at the site of infection (Andriole et al 1974, Power et al 1990, Widmer et al 1990, Lasierra et al 1994 or sterile intramedullary silicone catheters in tibiae (Mayberry-Carson et al 1992), but they do not always cause a persistent infection. To improve infection rates, precolonized implants have been used alone with intra-peritoneal implantation (Ward et al 1992), or together with a bacterial suspension in orthopedic surgery models (Barth et al 1989, Gracia et al 1998.…”

Section: Discussionmentioning

confidence: 99%

“…After the rst S. aureus infection model was developed (Rodet 1973), the disease was reproduced in rabbits (Andriole et al 1974, Mayberry-Carson et al 1984, Norden and Budinsky 1990, Lambe et al 1991, Lasierra et al 1994) and rats (Power et al 1990). Infection has been reproduced by systemic inoculation of bacterial suspensions (Lasierra et al 1994) or direct introduction of bacteria at the site of the implant, in suspension (Lambe et al 1991, Mayberry-Carson et al 1992, Lasierra et al 1994, in suspension and in bio lm (mixed models; Barth et al 1989, Gracia et al 1998 or in bio lm alone (Ward et al 1992).…”

mentioning

confidence: 99%

Evaluation of four experimental osteomyelitis infection models by using precolonized implants and bacterial suspensions

Monzón¹,

García-Álvarez²,

Laclériga³

et al. 2002

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

“…The rabbit tibia model is a well-established one for the experimental evaluation of implant-related infection and osteomyelitis in orthopedic research. 7,8,12,[48][49][50][51] One of the main reasons for investigating this problem in an experimental model was to eliminate those influences on infection that result from a fracture or softtissue trauma. In clinical settings, these influences are heterogeneous and, thus, a handicap in comparative studies.…”

Section: Discussionmentioning

confidence: 99%

Polylactide implants and bacterial contamination: An animal study

Mainil‐Varlet¹,

Hauke²,

Maquet³

et al. 2000

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Although bioresorbable aliphatic polyesters derived from lactic acid are now used clinically as sutures, bone-fracture fixation devices and sustained-release drug-delivery systems, very little is known about their behavior in the infected environment. The aim of the present study was to compare the resistance to infection of two polylactide implants with different degradation characteristics, and to evaluate the influence of a bacterial challenge on their mechanical and physicochemical properties. Various quantities of a beta-haemolyzing strain of Staphylococus aureus (V 8189-94) were inoculated into the medullary cavity of rabbit tibiae, and an extruded polylactide rod composed of either P(L)LA (Poly(L-Lactide)) or P(L/DL)LA (Poly(L/DL-Lactide)) was then inserted. Animals were sacrificed four weeks after surgery. The tibiae and implants were removed under sterile conditions and evaluated microbiologically by culturing. The severity of infection was graded according to positive colony-forming units in the bone. The mechanical properties of the retrieved implants were assessed by 4-point bending and shear tests, performed in compliance with the ASTM D790 standard and their physicochemical characteristics also were characterized. P(L)LA and P(L/DL)LA implants were equally resistant to local infection, their mechanical and physicochemical properties being unaffected by bacterial challenge. Hence, once an infection has become established, the release of bactericidal/bacteriostatic by-products during implant degradation does not appear to affect its natural course. The release of bactericidal/bacteriostatic degradation products at the implantation site is unlikely to affect the natural course of an established infection.

show abstract

Osteomyelitis Experimentally Induced With Bacteroides thetaiotaomicron and Staphylococcus epidermidis

Cited by 18 publications

References 0 publications

Potential of bone scaffolds containing vancomycin and bone morphogenetic protein-2 in a rat model of osteomyelitis

Potential of bone scaffolds containing vancomycin and bone morphogenetic protein-2 in a rat model of osteomyelitis

Evaluation of four experimental osteomyelitis infection models by using precolonized implants and bacterial suspensions

Polylactide implants and bacterial contamination: An animal study

Contact Info

Product

Resources

About