Osteonectin ? a differentiation marker of bone cells

Jundt, Gernot; Berghäuser, K. H.; Termine, John D.; Schulz, Andreas

doi:10.1007/bf00218209

Cited by 129 publications

(60 citation statements)

References 18 publications

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“…This fact was demonstrated by immunolocalisation of low levels of ON to blood vessels and soft tissue. It has been reported from immunohistochemical studies that young osteocytes express ON (Jundt et al, 1987), but little immunolocalisation of ON to osteocytes was observed in this study with adult human bone. Although BSP and OPN were both immunolabelled with polyclonal antisera, anti-BSP demonstrated much weaker immunolabelling than anti-OPN, even though both proteins are structurally similar and contain the RGD domain.…”

Section: Discussioncontrasting

confidence: 79%

“…Immunolocalisation of osteonectin (ON) (Jundt et al, 1987;Bianco et al, 1988;Park et al, 1996), alkaline phosphatase (ALP) (Bronckers et al, 1987), osteocalcin (OC) (Bronckers et al, 1987;Bronckers et al, 1994;Stafford et al, 1994;Semba et al, 2000;Rauch et al, 2000;Miao et al, 2001) bone sialoprotein (BSP) (Miao et al, 2001) and OC in situ hybridisation (Arai et al, 1993) were all performed in paraffin-embedded decalcified bone. The major disadvantage however of paraffin embedding as compared with plastic embedding is that it requires bone decalcification, a time-consuming process that eliminates essential information about mineralisation and locations of recent bone formation.…”

Section: Technovit 9100 New® Embeddingmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemistry of matrix markers in Technovit 9100 New®-embedded undecalcified bone sections

Yang¹,

Davies²,

Archer³

et al. 2003

eCM

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Trabecular bone is routinely analysed by histomorphological-histometrical and immunohistochemical techniques as means of assessing the differentiation status of bone deposition and growth. Currently few embedding resins exist for which both morphological and immunohistochemical analyses can be performed on mineralised tissue. Paraffin, the standard embedding medium for bone enzyme and immunohistochemistry, can only be used on demineralised tissue, but then trabecular structure may be badly preserved. Methyl methacrylate (MMA), the resin of choice for undecalcified bone histology can only be used for bone immunohistochemistry if the usual, highly exothermic polymerisation procedure is avoided which destroys both, enzyme activity and tissue antigenicity. Consequently, most current practices involve cutting samples in half to be processed in separate embedding media when more than one type of analysis is required. Technovit 9100 New® is a low temperature MMA embedding system that is purported to significantly improve tissue antigenicity preservation allowing polymerisation at -20°C. In this study, Technovit 9100 New®-embedded undecalcified trabecular bone samples (adult human, young bovine and ovine) yielded immunolabelling with several bone matrix markers and preserved morphological features in 7µm sections when stained with Masson-Goldner, von Kossa, or toluidine blue. Bone samples from all resins used (routine MMA, LR White, Technovit 9100 New®) were immunolabelled with antibodies against osteocalcin, alkaline phosphatase, osteopontin, osteonectin, bone sialoprotein and procollagen type I amino-terminal propeptide. Technovit 9100 New® -embedded bone yielded more reliable immunolabelling of the matrix proteins when compared with heat or cold-cured LR White or standard embedded MMA samples. Technovit 9100 New® provided better routine histology than LR White, and was comparable to MMA. Results demonstrated that Technovit 9100 New® can be used as a low-temperature acrylic resin embedding method for routine undecalcified bone histology, as well as for immunohistochemistry.

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Section: Discussioncontrasting

confidence: 79%

Section: Technovit 9100 New® Embeddingmentioning

confidence: 99%

Immunohistochemistry of matrix markers in Technovit 9100 New®-embedded undecalcified bone sections

Yang¹,

Davies²,

Archer³

et al. 2003

eCM

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“…Similar to TIMP RNA, alkaline phosphatase protein was found in more mature osteoblasts, as well as in preosteoblasts. Descriptions of the pattern of expression of other genes (osteopontin, osteonectin, osteocalcin) during osteogenesis suggests that hXBP-1 expression also correlates relatively well with that of osteonectin (SPARC) (Nomura et al, 1988;Holland et al, 1987;Metaranta et al, 1989; Mundlos et Jundt et al, 1987), except that this gene is expressed in hypertrophic chondrocytes, whereas hXBP-1 and TIMP are expressed principally in matrix secreting chondrocytes of the mature zone of the growth cartilage.…”

Section: Discussionmentioning

confidence: 99%

In situ hybridization studies suggest a role for the basic region‐leucine zipper protein hXBP‐1 in exocrine gland and skeletal development during mouse embryogenesis

Clauss

Gravallese

Darling

et al. 1993

Developmental Dynamics

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The spatial and temporal distribution of transcripts for the TREERE-binding basic region-leucine zipper protein hXBP-1 was determined by in situ hybridization. Analysis of embryos from day 10.5 to 18.5 pc revealed high level expression of hXBP-1 RNA in two developing organ systems: 1) in bone and cartilage cells of the developing skeleton and toothbuds, and 2) in exocrine glands including the pancreas and the submandibular and salivary glands. High level expression was also found in whisker follicles and in selected cells in brown adipose tissue. In the developing skeleton, hXBP-1 RNA was expressed starting on day 11.5 pc in osteoblasts of newly formed intramembranous bone. Thereafter, hXBP-1 was expressed in both osteoblasts and preosteoblasts in bone formed directly by intramembranous formation as well as in bone formed during endochondral ossification. The most intense signal was observed in preosteoblasts and osteoblasts of newly forming bone. At day 11.5 pc low level hXBP-1 expression was also observed in matrix secreting chondroblasts of bones which are formed initially of cartilage, at the stage where they consist entirely of cartilage. Signal was also present in matrix producing chondroblasts of the mature zone of the growth region during endochondral ossification although at significantly lower level than in osteoblasts. hXBP-1 is thus the first transcription factor described, to our knowledge, whose level of expression is modulated during the osteoblast developmental sequence in vivo. The pattern of expression of hXBP-1 in the developing skeleton was found to be very similar to that of the genes encoding the tissue inhibitor of metalloproteinase and alkaline phosphatase throughout development. These observations suggest that hXBP-1 may play a role in regulating the expression of tissue specific genes (TIMP, osteonectin, osteopontin, osteocalcin) expressed in osteoblasts. It is intriguing that the promoter regions of several such genes contain po-

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“…Osteonectin, a recently discovered non-collagenous protein in bone, is a phosphoprotein synthesized by osteoblasts, but it is not a specific bone protein (Termine 1983, Jundt et al 1987, Bianco et al 1988, Tracy et al 1988. Osteocalcin, or bone Gla protein, is vitamin K dependently synthesized by osteoblasts.…”

Section: Methodsmentioning

confidence: 99%