Osteopontin and bone metabolism in healing cranial defects in rabbits

Effects of fibrin-binding oligopeptide on osteopromotion in rabbit calvarial defects

“…They detected a significant difference in the percentage of new bone formation between peptide-coated group and peptide-uncoated group: 6.60 ± 0.89% and 23.72 ± 1.73% at 2 weeks, 34.77 ± 3.31% and 42.27 ± 2.35% at 4 weeks, respectively. On the contrary, Gordjestani et al [33] investigated the influence of osteopontin on bone repair in rabbit calvarial defects. There were no statistically significant differences in total bone formation between defects filled with osteopontin-hydroxyapatite and those with hydroxyapatite only.…”

Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces

“…A group of genetically linked bone proteins with similar structure and function, called small integrin binding ligand N-glycosolated proteins (SIBLINGs), are considered important in the generation and remodelling of skeletal tissue [4] and have in the past attracted considerable interest as biomolecular components in functional coatings that aim to improve the performance of orthopaedic materials for guided tissue engineering [5][6][7][8].…”

“…Numerous in vitro cell assays on different synthetic materials coated with OPN have been studied in the past with the purpose to explore whether OPN may influence the functionality of biomaterial surfaces [5,6,[15][16][17]. For instance, it is found that OPN on hydrophilic polystyrene surfaces enhance the adhesion and spreading area of MG63 cells [15] and that the amine endgroups enhance the OPN mediated adhesion and spreading area of bovine endothelial cells as compared to carboxylic, hydroxylic and methylic functionalized surfaces [8].…”

“…It was shown that OPN covalently immobilized on poly(hydroxyethyl methacrylic acid) (poly(HEMA)) increase the adhesion of bovine endothelial cells as compared to pure poly(HEMA) [17]. Previous studies [5,6,[15][16][17] thus indicate that OPN may act as a functional coating on orthopaedic implants, e.g. for joint prostheses to improve remodelling of bone tissue around biomedical implants.…”

“…Changes in hardness and relative mass of mineralized tissue have been reported as well as the speed of bone remodelling and vascularisation in OPN-knockout mice [19][20][21][22]. OPN coated coralline porous HA granulae was used in a rabbit cranial bone substitute model [6], where a positive effect of OPN on bony ingrowth from the periphery of the surgically produced defect in the rabbit crania was observed, whereas no effect in bony growth on the granulae was found. Besides a reported bone regenerative effect OPN is also found to have an inhibiting effect on the adversative foreign body reaction to implants [7,23].…”