Osteoporosis and polymorphisms of osteoprotegerin gene in postmenopausal women – a pilot study

Cvijetić, Selma; Grazio, Simeon; Kosović, Pašezada; Uremović, Melita; Nemčić, Tomislav; Bobić, Jasminka

doi:10.5114/reum.2016.58755

Cited by 13 publications

(10 citation statements)

References 13 publications

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“…Finally, in a recent study of randomly selected 22 postmenopausal women and 59 women without osteoporosis, there was a trend for A163G polymorphism in terms of a positive correlation with higher bone loss. Furthermore, the results of their study indicated that a significantly greater number of women with osteoporosis had AG polymorphism compared to women without osteoporosis, while no significant difference was found in the prevalence of TT and GG polymorphisms between patients with and without osteoporosis (15). We also found similar results in the present study.…”

Section: Discussionsupporting

confidence: 87%

“…Growing evidence suggest that OPG is among the most important candidate genes associated with the pathogenesis of osteoporosis (5,12). To date, several single nucleotide polymorphisms (SNPs) in OPG have been identified, including A163G, T245G, T950C and G1181C, which have been evaluated in terms of their relationship with osteoporosis and BMD (5,7,8,(13)(14)(15). Several genes among these have been assumed to influence BMD determination; however, the magnitude of impact and the exact role of these genes remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Association analysis between A163G and T245G gene polymorphisms of osteoprotegerin and bone mineral density in Turkish postmenopausal women

Palabıyık¹,

Özdemir²,

Tokuc³

et al. 2018

MJWBS

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Osteoprotegerin (OPG) is one of the most important candidate genes associated with osteoporosis predisposition. The aim of the present study was to evaluate the relationship between bone mineral density (BMD) and two polymorphisms in the OPG promoter, namely A163G and T245G among postmenopausal women. Material and Methods: A total of 109 women with postmenopausal osteoporosis and 85 healthy controls were included in the study. BMD of L1, L4, total lumbar spine (L1-4), neck, hip and total hip were evaluated by means of dual-energy X-ray absorptiometry. A163G and T245G polymorphisms were determined by PCR and RFLP. Results: The frequencies of genotypes were as follows: AG+GG (46.8%), AA (53.2%) for A163G and GG+TG (36.7%), TT (63.3%) for T245G. Compared to healthy women, remarkably more women with osteoporosis were found to have AG+GG (p=0.005) and GG+TG (p=0.049). Significant associations were observed between the genotypes and BMD of lumbar spine for the T245G and A163G polymorphisms in postmenopausal women and controls (p˂0.05 for all). The GG+TG genotypes correlated with lower BMD compared to TT for T245G. Similarly, for A163G, the AG+GG genotypes were associated with lower BMD compared to AA. Conclusion: Our results suggest that T245G and A163G polymorphisms in the OPG promoter may contribute to the genetic regulation of BMD. These results are expected to be beneficial for further studies investigating the role of OPG in osteoporosis.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Association analysis between A163G and T245G gene polymorphisms of osteoprotegerin and bone mineral density in Turkish postmenopausal women

Palabıyık¹,

Özdemir²,

Tokuc³

et al. 2018

MJWBS

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“…Previous studies have indicated that osteoporosis and BMD are heritable ( 6 , 7 ), and >60 susceptible loci have been found to be associated with osteoporosis and BMD ( 6 ). Among these, polymorphisms of several genes have been found to be involved in PMOP, including tumor necrosis factor (TNF)-α, interleukin (IL)10, osteoprotegerin, estrogen receptor 1 gene, estrogen receptor α, cannabinoid receptor 2, vitamin D receptor gene and LDL receptor related protein 5 ( 8 – 14 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of aberrantly expressed long non-coding RNAs in postmenopausal osteoporosis

Bai

Lin

et al. 2018

Int J Mol Med

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Postmenopausal osteoporosis (PMOP) is a common skeletal disorder in postmenopausal women. The present study aimed to identify the key long non-coding RNAs (lncRNAs) in PMOP through RNA sequencing. RNA sequencing was performed to obtain the expression profile of lncRNAs and mRNAs in blood samples of patients with PMOP and normal controls (NCs). Following the identification of differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs), the DElncRNA-DEmRNA co-expression network was constructed. A search was performed for the DEGs transcribed within a 100-kb window upstream or downstream of DElncRNAs, which served as nearby DEmRNAs of DElncRNAs. Functional annotation of the DEmRNAs co-expressed with DElncRNAs was performed. The GSE56815 dataset was used to verify the expression of selected DEmRNAs and DElncRNAs. Three blood samples from patients with PMOP and two blood samples from NCs were used for RNA sequencing. Compared with the NC group, a total of 185 DEmRNAs and 51 DElncRNAs were obtained in PMOP. A total of 3,057 co-expression DElncRNA-DEmRNA pairs and 97 DElncRNA-nearby DEmRNA pairs were obtained. Six DEmRNAs [diacylglycerol O-acyltransferase 2, potassium voltage-gated channel subfamily S member 1, peptidase inhibitor 3, secretory leukocyte peptidase inhibitor, galectin-related protein and alkaline phosphatase, liver/bone/kidney (ALPL)] were nearby co-expressed genes of four DElncRNAs, including LOC105376834, LOC101929866, LOC105374771 and LOC100506113. Three PMOP-associated DEmRNAs, including ALPL, suppressor of cytokine signaling 3 and adrenomedullin, were co-expressed with the hub DElncRNAs (LINC00963, LOC105378415, LOC105377067, HCG27, LOC101928143 and LINC01094) of the positively and negatively co-expressed DElncRNA-DEmRNA interaction network. The expression of selected DEmRNAs and DElncRNAs was consistent with the RNA-sequencing results. In conclusion, the present study identified the key DEmRNAs and DElncRNAs in PMOP, which may provide clues for understanding the mechanism and developing novel biomarkers for PMOP.

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“…Two meta-analysis study found G allele of the OPG 163A>G polymorphisms increased osteoporosis risk in Caucasians whereas allele C of the OPG 1181G>C decreased osteoporosis risk mainly in Asians (31,32). A pilot study by Cvijetic showed that postmenopausal women with osteoporosis had AG genotype (of the 163A>G) polymorphism more frequently than women without osteoporosis while the prevalence of TT and GG polymorphism between patients with and without osteoporosis was not significantly different (35). Langdahl et al (23) reported that in SNP 163A>G, G alleles were more commonly found in subjects with osteoporosis than normal.…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women

Haryono

Tulaar²,

Sudoyo

et al. 2019

Tod

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Amaç: Reseptör aktivatör nükleer faktör-B ligandı (RANKL) ve osteoprotegerinin (OPG) kemik yeniden modelleme ve osteoporozda önemli bir rolü olduğu gösterilmiştir. Bu çalışmada, Endonezya menopoz sonrası kadınlarda düşük kemik mineral yoğunluğu (KMY) ile RANKL ve OPG genlerinin polimorfizmleri (sırasıyla TNFSF11 ve TNFRSF11B) arasındaki ilişkinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Elli-altmış beş yaş arası 60 postmenopozal kadın uygun bulundu. KMY, çift-enerjili X-ışını absorpsiyometrisi kullanılarak ölçülmüştür. TNFSF11 ve TNFRSF11B'nin genotipleri polimeraz zincir reaksiyonu-kısıtlama fragman uzunluğu polimorfizmleri ve DNA dizileme yöntemleri ile elde edildi. TNFSF11'den (-290C> T,-643C> T,-693G> C) ve TNFRSF11B'den (163A>G, 950T>C, 1181G>C) üç tek nükleotid polimorfizmleri (SNP) seçildi. Allel dağılımı ile KMY arasındaki ilişki ki-kare veya Fischer kesin testi kullanılarak hesaplandı. Kemik bölgelerindeki tüm SNP'leri ve BMD'yi analiz etmek için çoklu lojistik regresyon kullanıldı. Anlamlılık p<0,05 olarak ayarlandı. Bulgular: Çoğu birey daha düşük KMY'ye (%83,3) sahipti. Sağlıklı ve düşük KMY arasındaki bireylerin özellikleri karşılaştırılabilir bulundu (hepsi p>0,05). TNSSF11 ve TNFRSF11B'de genotiplerin ve allellerin sağlıklı ve düşük KMY'ler arasındaki dağılımı anlamlı olarak farklı değildi (hepsi p>0,05). Tüm kemik bölgelerinde TNFSF11 ve TNFRSF11B'nin SNP'leri ile KMY arasında ilişki yoktu (hepsi p>0,05). Objective: Prior studies have shown that receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) have an essential role in bone remodeling and osteoporosis. This study aimed to investigate the association between RANKL (TNFSF11) and OPG (TNFRSF11B) genes' polymorphisms with low bone mineral density (BMD) in Indonesian postmenopausal women. Materials and Methods: Sixty postmenopausal women aged between 50-65 years were eligible. The BMD was measured by using dualenergy X-ray absorptiometry. The genotypes of TNFSF11 and TNFRSF11B were obtained by polymerase chain reaction-restriction fragment length polymorphisms and DNA sequencing methods. Three single nucleotide polymorphisms (SNPs) from TNFSF11 (-290C>T,-643C>T,-693G>C) and from TNFRSF11B (163A>G, 950T>C, 1181G>C) were selected. The association between alleles distribution and BMD was computed using chi-square or Fischer's exact test. Multiple logistic regression was used to analyze between all SNPs and BMD at bone sites. Significance was set at p<0.05. Results: Most subjects had lower BMD (83.3%). Characteristics of subjects between healthy and low BMD were comparable (all p>0.05). The distribution of genotypes and alleles in TNSSF11 and TNFRSF11B between healthy and low BMD were not significantly different (all p>0.05). There was no association between SNPs of TNFSF11 and TNFRSF11B with BMD at all bone sites (all p>0.05). Conclusion: The present study suggests that TNFSF11 and TNFRSF11B gene polymorphisms are not associated with BMD in Indonesian postmenopausal women aged between 50-65 years old.

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Osteoporosis and polymorphisms of osteoprotegerin gene in postmenopausal women – a pilot study

Cited by 13 publications

References 13 publications

Association analysis between A163G and T245G gene polymorphisms of osteoprotegerin and bone mineral density in Turkish postmenopausal women

Association analysis between A163G and T245G gene polymorphisms of osteoprotegerin and bone mineral density in Turkish postmenopausal women

Identification of aberrantly expressed long non-coding RNAs in postmenopausal osteoporosis

Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women

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