Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits

Wei, Bing; Wang, Chao; Yan, Cheng; Tang, Bushun; Yu, Xiaofei; Zhang, Hui; Tang, Lixia; Wang, Qing

doi:10.1007/s00774-019-01078-w

Cited by 19 publications

(21 citation statements)

References 35 publications

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“…It has been demonstrated that it can increase the amount of new bone formation and improve the biomechanical strength by using injectable calcium phosphate cement (CPC) as a carrier to inject recombinant human BMP-2 into the tendon–bone interface of the rabbit anterior cruciate ligament reconstruction model [ 10 ]. However, it will cause a series of complications owing to much higher physiological level and the unreasonable release kinetics, such as local soft tissue edema, heterotopic ossification, bone resorption around the implant [ 11 , 12 ]. Therefore, it is necessary to solve the problems of carrier, optimal dose, and effective time of growth factor release in the human body if BMP-2 is widely used in the treatment of clinical rotator cuff injuries.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Exosome-delivered BMP-2 and polyaspartic acid promotes tendon bone healing in rotator cuff tear via Smad/RUNX2 signaling pathway

Liu¹,

Liu²,

Huang³

et al. 2022

Bioengineered

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Rotator cuff tear is the main form of shoulder joint injury, which seriously affects shoulder joint function. This study aimed to clarify the function and mechanism of exosomes containing polylactic acid (PLA), polylactic acid copolymer and BMP-2 in tendon bone healing of rotator cuff tear. First, CD44 expression in bone marrow mesenchymal stem cells (BMSCs) and CD90 and CD44 in exosomes were analyzed by flow cytometry. Then, stability and targeting identification of exosome-delivered bone morphogenetic protein (BMP)-2 and PLA microcapsules were measured by transmission electron microscopy (TEM), DiO/DiI staining. Finally, tendon-bone repair after acute rotator cuff rupture in rabbits was established, and the function of BMP-2 exosomes for tendon bone healing in rotator cuff tear was evaluated by micro-CT, biomechanical determination and histochemical staining methods. The results showed that the exosomes of polyaspartic acid-polylactic acid-glycolic acid copolymer (PASP-PLGA) microcapsules were successfully established which showed good stability and targeting. The bone mineral density (BMD), tissue mineral density (TMD) and bone volume fraction (BV/TV) were higher, while the stiffness and the ultimate load strength of the tendon interface were enhanced under treatment with exosomes of PASP-PLGA microcapsules. Histochemical staining showed that exosomes of PASP-PLGA microcapsules promoted tendon and bone interface healing after rotator cuff injury. The tendon regeneration- and cartilage differentiation-related protein expressions were significantly upregulated under treatment with exosomes of PASP-PLGA microcapsules. In conclusion, exosome-delivered BMP-2 and PLA promoted tendon bone healing in rotator cuff tear via Smad/RUNX2 pathway. Our findings may provide a new insight for promoting tendon healing.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Exosome-delivered BMP-2 and polyaspartic acid promotes tendon bone healing in rotator cuff tear via Smad/RUNX2 signaling pathway

Liu¹,

Liu²,

Huang³

et al. 2022

Bioengineered

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“…After ACLR using hamstring tendon grafts, bone tunnel widening has been reported, leading to graft loosening and the necessity for revision surgery. 9,46 At an early stage in ACLR, the bone tunnel reportedly exhibits transient widening, 48 and 1 study reported that bone tunnel widening may be prevented by MSC transplantation. 15 In the current study, bone tunnel widening was significantly prevented in the sheet group compared with the control group at 4 weeks postoperatively.…”

Section: Discussionmentioning

confidence: 99%

Adipose-Derived Stem Cell Sheets Improve Early Biomechanical Graft Strength in Rabbits After Anterior Cruciate Ligament Reconstruction

et al. 2021

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Background: Although various reconstruction techniques are available for anterior cruciate ligament (ACL) injuries, a long recovery time is required before patients return to sports activities, as the reconstructed ACL requires time to regain strength. To date, several studies have reported use of mesenchymal stem cells in orthopaedic surgery; however, no studies have used adipose-derived stem cell (ADSC) sheets in ACL reconstruction (ACLR). Hypothesis: ADSC sheet transplantation can improve biomechanical strength of the autograft used in ACLR. Study Design: Controlled laboratory study. Methods: A total of 68 healthy Japanese white rabbits underwent unilateral ACLR with a semitendinosus tendon autograft after random enrollment into a control group (no sheet; n = 34) and a sheet group (ADSC sheet; n = 34). At 2, 4, 8, 16, and 24 weeks after surgery, rabbits in each group were sacrificed to evaluate tendon-bone healing using histological staining, micro–computed tomography, and biomechanical testing. At 24 weeks, scanning transmission electron microscopy of the graft midsubstance was performed. Results: The ultimate failure load for the control and sheet groups, respectively, was as follows: 17.2 ± 5.5 versus 37.3 ± 10.3 ( P = .01) at 2 weeks, 28.6 ± 1.9 versus 47.4 ± 10.4 ( P = .003) at 4 weeks, 53.0 ± 14.3 versus 48.1 ± 9.3 ( P = .59) at 8 weeks, 66.2 ± 9.3 versus 95.2 ± 43.1 ( P = .24) at 16 weeks, and 66.7 ± 27.3 versus 85.3 ± 29.5 ( P = .39) at 24 weeks. The histological score was also significantly higher in the sheet group compared with the control group at early stages up to 8 weeks. On micro–computed tomography, relative to the control group, the bone tunnel area was significantly narrower in the sheet group at 4 weeks, and the bone volume/tissue volume of the tendon-bone interface was significantly greater at 24 weeks. Scanning transmission electron microscopy at 24 weeks indicated that the mean collagen fiber diameter in the midsubstance was significantly greater, as was the occupation ratio of collagen fibers per field of view, in the sheet group. Conclusion: ADSC sheets improved biomechanical strength, prevented bone tunnel enlargement, and promoted tendon-bone interface healing and graft midsubstance healing in an in vivo rabbit model. Clinical Relevance: ADSC sheets may be useful for early tendon-bone healing and graft maturation in ACLR.

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“…Organic MacPSs mainly consists of COL sponges. They have been studied for the release of BMP2, alone or with other drugs/proteins such as alendronate which promotes osteogenic cells differentiation and supresses osteoclasts [102], or osteoprotegerin which inhibits osteoclast maturation, leading to the inhibition of new bone resorption [189]. It has also been used to compare the efficiency of BMP2 and BMP9 for bone regeneration [190].…”

Section: V111 Single Formulation: Organic and Inorganic Material Alone Or In Combinationmentioning

confidence: 99%

“…Finally, the ACL reconstruction model has been used in NZ white rabbit to check the ability of osteoprotegerin/BMP2-loaded COL sponge to promote tendon-bone healing [189]. This tendon-bone healing was improved with the reduction of bone tunnel enlargement and an enhanced formation of fibrocartilage and COL fibers at the tendon-bone interface.…”

Section: Vii211 Macroporous Scaffoldsmentioning

confidence: 99%

Design and applications of protein delivery systems in nanomedicine and tissue engineering

Bizeau

Mertz

2021

Advances in Colloid and Interface Science

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Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits

Cited by 19 publications

References 35 publications

RETRACTED ARTICLE: Exosome-delivered BMP-2 and polyaspartic acid promotes tendon bone healing in rotator cuff tear via Smad/RUNX2 signaling pathway

RETRACTED ARTICLE: Exosome-delivered BMP-2 and polyaspartic acid promotes tendon bone healing in rotator cuff tear via Smad/RUNX2 signaling pathway

Adipose-Derived Stem Cell Sheets Improve Early Biomechanical Graft Strength in Rabbits After Anterior Cruciate Ligament Reconstruction

Design and applications of protein delivery systems in nanomedicine and tissue engineering

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