Osteosarcoma contains a subpopulation of cancer stem-like cells that are highly resistant to radiotherapy

Neves, Sara Rm; Lopes, Aurio Og; Carmo, Anália do; Abrunhosa, Antero; Simões, P.C.P.S.; Paiva, Artur; Botelho, Maria Filomena

doi:10.1186/1753-6561-4-s2-p40

Cited by 2 publications

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“…Recent studies performed in our laboratory have identified a subset of cells with stem-like properties in OS that is relatively resistant to conventional chemotherapeutic agents and to ionizing radiation [8,9]. In this work we aim to identify the molecular mechanisms underlying the drug resistance phenotype exhibited by CSCs.…”

Section: Goalsmentioning

confidence: 99%

Elucidation of the drug resistance mechanisms of osteosarcoma cancer stem cells with PET tracers

Neves

Oliveira

Carmo

et al. 2011

1st Portuguese Biomedical Engineering Meeting

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Cells stem cells (CSCs) have been identified in several types of malignancies and referred as the responsible for driving tumor growth and resistance to chemotherapy. Previous studies have identified CSCs in a human osteosarcoma cell line that is relatively resistant to doxorubicin. We aimed to identify the mechanisms underlying this resistant phenotype and to assess the functional alterations occurring during differentiation of CSCs using PETradiotracers. CSCs were isolated using the sphere-formation assay and incubated with different concentrations of DOX during 48h, with and without verapamil. Cells' viability was measured using the MTT-colorimetric assay. Metabolic and osteoblastic activity was assessed with [ 18 F]FDG and [ 18 F]NaF, respectively during differentiation of CSCs. The half-maximal inhibitory concentrations of DOX was higher in CSCs (IC50=0.90±0.10μM) than in the MNNG/HOS (IC50=0.61±0.05μM) and decreased to 0.29μM with verapamil. Cellular uptake of [ 18 F]FDG was lower in CSCs and increased progressively during differentiation, whereas [ 18 F]NaF accumulation was higher in undifferentiated CSCs.

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Section: Goalsmentioning

confidence: 99%