2017
DOI: 10.4236/cm.2017.81001
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OSW-1 Induced Apoptosis in Hepatocellular Carcinoma through Generation of ROS, Cytochrome C and Noxa Activation Independent of p53 with Non-Activation of Caspase-3

Abstract: Aim: To study the antitumor mechanism of OSW-1 in hepatocellular carcinoma. Materials and Methods: The expression profiling microarray was carried out to extract RNA from SK-Hep-1 which suffered from OSW-1. ρ 0 -SK-Hep-1 was maintained SK-Hep-1 in MEM containing 100 μg/L ethidium bromide (EB), 1 mM sodium pyruvate and 50 μg/ml uridine for 40 days. Then confirmed COX-I and COX-II of mitochondrial DNA were knocked out. Cells suffered from OSW-1 or doxorubicin. Then cells were washed twice with cold PBS and incub… Show more

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Cited by 3 publications
(4 citation statements)
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“…The final outcome will be the apoptotic cell death of the TCT treated-cancer cells [81], consistent with decreased density of TCT treated nicotine-induced mitochondria as reported previously [24]. Nevertheless, there are also some contradicting reports that question the role of caspases and modulation of Bax/Bcl-2 in the TCT executed apoptotic pathway [87,88]. However, the possibility that TCT might exhibit a different cellular mechanism of apoptosis in different cell types induced by different kinds of ROS warrants further investigation.…”
Section: Iv) Tocotrienols Trigger Major Apoptosis Pathway Involving Csupporting
confidence: 84%
“…The final outcome will be the apoptotic cell death of the TCT treated-cancer cells [81], consistent with decreased density of TCT treated nicotine-induced mitochondria as reported previously [24]. Nevertheless, there are also some contradicting reports that question the role of caspases and modulation of Bax/Bcl-2 in the TCT executed apoptotic pathway [87,88]. However, the possibility that TCT might exhibit a different cellular mechanism of apoptosis in different cell types induced by different kinds of ROS warrants further investigation.…”
Section: Iv) Tocotrienols Trigger Major Apoptosis Pathway Involving Csupporting
confidence: 84%
“…To detect ρ 0 SK-Hep1completely lacking mtDNA, using COX-I and COX-II upstream primer, downstream primer. The result was detected by agarose gel electrophoresis.See our previous research for details [6]. 4) RNA isolation, cDNA labeling Synthesis and Labeling and Expression profiling using microarray.…”
Section: )mentioning
confidence: 99%
“…The Labelled cRNAs were hybridized to the NimbleGen Human Gene Expression. See our previous research for details [6]. housekeeping genes, but only if the difference in the average values between the two groups to be compared is less than one cycle.…”
Section: )mentioning
confidence: 99%
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