Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity

Verdejo-Torres, Odette; Flores-Maldonado, Catalina; Padilla‐Benavides, Teresita; Campos-Blázquez, Jessica; Larre, Isabel; Lara-Lemus, Roberto; Salazar, Eduardo Pérez; Cereijido, Marcelino; Contreras, Rubén G.

doi:10.1007/s00232-019-00066-5

Cited by 5 publications

(2 citation statements)

References 60 publications

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“…The monolayers were then scratch-wounded using a sterile 200 μl pipette tip and suspended cells were washed away with PBS. The progress of cell migration into the wound was monitored every 24 h until wound closure using the ×10 objective of an Echo Rebel Microscope as previously described (43)(44)(45). The bottom of the plate was marked for reference, and the same field of the monolayers was photographed immediately after performing the wound (time = 0 h) and at different time-points after performing the scratch, as indicated in the figures.…”

Section: Methodsmentioning

confidence: 99%

Functional effect of indole-3 carbinol in the viability and invasive properties of cultured cancer cells

Baez-Gonzalez,

Carrazco-Carrillo,

Figueroa-Gonzalez

et al. 2023

Preprint

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Cancer treatment typically involves multiple strategies, such as surgery, radiotherapy, and chemotherapy, to remove tumors. However, chemotherapy often causes side effects, and there is a constant search for new drugs to alleviate them. Natural compounds are a promising alternative to this problem. Indole-3-carbinol (I3C) is a natural antioxidant agent that has been studied as a potential cancer treatment. I3C is an agonist of the aryl hydrocarbon receptor (AhR), a transcription factor that plays a role in the expression of genes related to development, immunity, circadian rhythm, and cancer. In this study, we investigated the effect of I3C on cell viability, migration, invasion properties, as well as mitochondrial integrity in hepatoma, breast, and cervical cancer cell lines. We found that all tested cell lines showed impaired carcinogenic properties and alterations in mitochondrial membrane potential after treatment with I3C. These results support the potential use of I3C as a supplementary treatment for various types of cancer.

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Section: Methodsmentioning

confidence: 99%

Functional effect of indole-3 carbinol in the viability and invasive properties of cultured cancer cells

Baez-Gonzalez,

Carrazco-Carrillo,

Figueroa-Gonzalez

et al. 2023

Preprint

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show abstract

“…e same treatment influences the apical/basolateral polarity, as reflected in the acceleration induced by ouabain in the ciliogenesis of the cells that develop a mature monolayer [40]. More recently, it has been shown that ouabain 10 nM accelerates collective cell migration of MDCK in wound healing assays [41]. In addition to demonstrating the influence of ouabain in the processes already described, we have shown that Na-K-ATPase is the primary receptor that mediates a signaling cascade involving c-Src and ERK1/2 [42].…”

Section: Introductionmentioning

confidence: 99%

Influence of Endogenous Cardiac Glycosides, Digoxin, and Marinobufagenin in the Physiology of Epithelial Cells

Toro

Jimenez

Hinojosa

et al. 2019

Cardiology Research and Practice

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Cardiac glycosides are a group of compounds widely known for their action in cardiac tissue, some of which have been found to be endogenously produced (ECG). We have previously studied the effect of ouabain, an endogenous cardiac glycoside, on the physiology of epithelial cells, and we have shown that in concentrations in the nanomolar range, it affects key properties of epithelial cells, such as tight junction, apical basolateral polarization, gap junctional intercellular communication (GJIC), and adherent junctions. In this work, we study the influence of digoxin and marinobufagenin, two other endogenously expressed cardiac glycosides, on GJIC as well as the degree of transepithelial tightness due to tight junction integrity (TJ). We evaluated GJIC by dye transfer assays and tight junction integrity by transepithelial electrical resistance (TER) measurements, as well as immunohistochemistry and western blot assays of expression of claudins 2 and 4. We found that both digoxin and marinobufagenin improve GJIC and significantly enhance the tightness of the tight junctions, as evaluated from TER measurements. Immunofluorescence assays show that both compounds promote enhanced basolateral localization of claudin-4 but not claudin 2, while densitometric analysis of western blot assays indicate a significantly increased expression of claudin 4. These changes, induced by digoxin and marinobufagenin on GJIC and TER, were not observed on MDCK-R, a modified MDCK cell line that has a genetically induced insensitive α1 subunit, indicating that Na-K-ATPase acts as a receptor mediating the actions of both ECG. Plus, the fact that the effect of both cardiac glycosides was suppressed by incubation with PP2, an inhibitor of c-Src kinase, PD98059, an inhibitor of mitogen extracellular kinase-1 and Y-27632, a selective inhibitor of ROCK, and a Rho-associated protein kinase, indicate altogether that the signaling pathways involved include c-Src and ERK1/2, as well as Rho-ROCK. These results widen and strengthen our general hypothesis that a very important physiological role of ECG is the control of the epithelial phenotype and the regulation of cell-cell contacts.

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Functional effect of indole-3 carbinol in the viability and invasive properties of cultured cancer cells

Baez-Gonzalez,

Carrazco-Carrillo,

Figueroa-Gonzalez

et al. 2023

Biochemistry and Biophysics Reports

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Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity

Cited by 5 publications

References 60 publications

Functional effect of indole-3 carbinol in the viability and invasive properties of cultured cancer cells

Functional effect of indole-3 carbinol in the viability and invasive properties of cultured cancer cells

Influence of Endogenous Cardiac Glycosides, Digoxin, and Marinobufagenin in the Physiology of Epithelial Cells

Functional effect of indole-3 carbinol in the viability and invasive properties of cultured cancer cells

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