Outbreak of MAV‐type barley yellow dwarf virus on wheat in the Garhwal Hills in India

Khetarpal, R. K.; Kumar, Jitesh; Beuve, Monique; Parakh, D. B.; Nath, Ram

doi:10.1111/j.1365-3059.1994.tb02705.x

Cited by 5 publications

(1 citation statement)

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“…In recent years, BYDV GAV-caused wheat yellow dwarf disease has observed throughout the northern and north-western regions of China. It was reported that outbreak of BYDV in field often coincided with a high population of viruliferous aphid vectors on the overwintered host plants during the most susceptible stage of the wheat crops and the changes in cultivation practices [ 11 ]. BYDV was shown to accumulate in phloem cells and BYDV viroplasm, virion aggregates and BYDV specific tubular structures were observed in both infected plant and aphid cells [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Development of monoclonal antibodies and serological assays specific for Barley yellow dwarf virus GAV strain

Chen

Liu

et al. 2015

Virol J

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BackgroundBarley yellow dwarf virus (BYDV) is one of the most devastating plant viruses and belongs to a ubiquitous plant virus group. In China, four BYDV strains (GPV, GAV, PAV and RMV) have been identified based on their specific aphid vectors and serological properties. Among the four identified strains, the GAV is the most common BYDV strain in China. To diagnose, forecast of BYDV GAV, two reliable serological assays for BYDV GAV detection were established.MethodsWe purified virion from a confirmed BYDV GAV source and used it as the immunogen to produce monoclonal antibodies against the virus. Using the hybridoma technology, three highly specific murine monoclonal antibodies were produced and two serological assays [antigen-coated-plate enzyme-linked immunosorbent assay (ACP-ELISA) and dot enzyme-linked immunosorbent assay (dot-ELISA)] were established for the BYDV GAV detection.ResultsAll three monoclonal antibodies reacted strongly and specifically with the BYDV GAV strain in crude leaf extracts. Titers of the monoclonal antibodies in ascitic fluids were up to 10−7 by indirect-ELISA. These three monoclonal antibodies (18A1, 18A9 and 12A11) all belonged to the isotype IgG1, kappa light chain. The highest dilution points for the three antibodies during the ACP-ELISA using infected crude leaf extracts were 1:163,840, 1:81,920 and 1:81,920 (w/v, g · mL−1), respectively. Result of dot-ELISA showed a successful detection of BYDV GAV strain in 1:5,120 (w/v, g · mL−1) diluted wheat leaf crude extracts. Analysis of 22 field wheat leaf samples and 33 aphid samples from the Shaanxi Province in China, using the two newly developed assays confirmed the presence of BYDV GAV in about 80 % of the wheat samples and 18 % of the aphid samples.ConclusionsAll three monoclonal antibodies are highly sensitive and specific to the BYDV GAV. The two newly developed serological assays are simple and effective. These two assays, particularly the dot-ELISA, are useful for high throughput detection of BYDV GAV in host plants and aphid vectors.

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Section: Introductionmentioning

confidence: 99%