Outbreak of metallo- -lactamase VIM-2-positive strains of Pseudomonas aeruginosa in the Ivory Coast

Jeannot, Katy; Guessennd, Nathalie; Fournier, Damien; Müller, Emeline; Gbonon, Valérie; Plésiat, Patrick

doi:10.1093/jac/dkt296

Cited by 20 publications

(10 citation statements)

References 10 publications

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“…Currently, the VIM family includes 41 variants, which have been mainly identified in P. aeruginosa but also in enterobacterial isolates. VIM‐2 is actually the most commonly reported MBL worldwide , with endemic spread in southern Europe (Greece, Spain, and Italy) and Southeast Asia (South Korea and Taiwan), but has also caused outbreaks in Africa, in particular in the Ivory Coast , South Africa , Tunisia , and some European countries, such as Germany , The Netherlands , and France . These outbreaks have mainly involved VIM‐producing P. aeruginosa , and rarely enterobacterial species.…”

Section: The Class B Metallo‐β‐lactamases (Mbls)mentioning

confidence: 99%

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide

Nordmann

Poirel

2014

Clinical Microbiology and Infection

620

497

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The spread of carbapenemase producers in Enterobacteriaceae has now been identified worldwide. Three main carbapenemases have been reported; they belong to three classes of β-lactamases, which are KPC, NDM, and OXA-48. The main reservoirs of KPC are Klebsiella pneumoniae in the USA, Israel, Greece, and Italy, those of NDM are K. pneumoniae and Escherichia coli in the Indian subcontinent, and those of OXA-48 are K. pneumoniae and Escherichia coli in North Africa and Turkey. KPC producers have been mostly identified among nosocomial isolates, whereas NDM and OXA-48 producers are both nosocomial and community-acquired pathogens. Control of their spread is still possible in hospital settings, and relies on the use of rapid diagnostic techniques and the strict implemention of hygiene measures.

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Section: The Class B Metallo‐β‐lactamases (Mbls)mentioning

confidence: 99%

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide

Nordmann

Poirel

2014

Clinical Microbiology and Infection

620

497

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“…aeruginosa outbreaks have been reported 5,6 . Worryingly, several of these have included metallo-β-lactamase (MBL) producing strains that are capable of rapidly hydrolysing carbapenems, a ‘last-resort’ treatment option for MDR Gram-negative infections 7–10 . MBL production is associated with higher mortality rates for P .…”

Section: Introductionmentioning

confidence: 99%

Dual β-lactam combination therapy for multi-drug resistant Pseudomonas aeruginosa infection: enhanced efficacy in vivo and comparison with monotherapies of penicillin-binding protein inhibition

Siriyong

Murray

Bidgood

et al. 2019

Sci Rep

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The aim of the study was to determine the efficacy of dual β-lactam combination treatments derived from eight approved drugs against Galleria mellonella larvae infected with MDR strains of P . aeruginosa . Carbapenem-resistant P . aeruginosa NCTC 13437 and an unrelated clinical isolate were used to infect G . mellonella larvae and the efficacy of twenty-eight dual β-lactam combination therapies were compared to their constituent monotherapies. For the most potent combinations identified, penicillin-binding protein (PBP) inhibition profiles were measured and compared with each constituent antibiotic. Five of the dual β-lactam combinations resulted in greater than 70% survival of infected G . mellonella . Two combinations showed potent, enhanced efficacy versus both strains − ceftazidime + meropenem and aztreonam + meropenem. Comparison of PBP inhibition profiles revealed that the enhanced efficacy of these two dual β-lactam combinations could not be explained by more potent inhibition of PBPs or inhibition of a broader range of PBPs. A possible contribution to the enhanced efficacy of the combinations could be stimulation of innate immunity via increased haemocyte numbers compared to their constituent monotherapies. Combinations of β-lactam antibiotics show promise in overcoming MDR P . aeruginosa and are worthy of additional study and development.

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“…The presence of VIM-2 has been established in Africa. Hence, a ST1488 isolate harboring bla VIM-2 has been reported in Abidjan (Ivory Coast) [27]. ST1488 belongs to the CC308, already associated with bla MBL [5].…”

Section: Discussionmentioning

confidence: 99%

Population Structure of Clinical Pseudomonas aeruginosa from West and Central African Countries

Cholley

Guyeux

et al. 2014

PLoS ONE

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Background Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called ‘high-risk clusters’. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted in Africa, where a very few isolates have been analyzed. Here, we characterized a collection of clinPA isolates from four countries of West and Central Africa.Methodology184 non-redundant isolates of clinPA from hospitals of Senegal, Ivory Coast, Nigeria, and Central African Republic were genotyped by MLST. We assessed their resistance level to antibiotics by agar diffusion and identified the extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) by sequencing. The population structure of the species was determined by a nucleotide-based analysis of the entire PA MLST database and further localized on the phylogenetic tree (i) the sequence types (STs) of the present collection, (ii) the STs by continents, (iii) ESBL- and MBL-producing STs from the MLST database.Principal FindingsWe found 80 distinct STs, of which 24 had no relationship with any known STs. ‘High-risk’ international clonal complexes (CC155, CC244, CC235) were frequently found in West and Central Africa. The five VIM-2-producing isolates belonged to CC233 and CC244. GES-1 and GES-9 enzymes were produced by one CC235 and one ST1469 isolate, respectively. We showed the spread of ‘high-risk’ international clonal complexes, often described as multidrug-resistant on other continents, with a fully susceptible phenotype. The MBL- and ESBL-producing STs were scattered throughout the phylogenetic tree and our data suggest a poor association between a continent and a specific phylogroup.ConclusionsESBL- and MBL-encoding genes are borne by both successful international clonal complexes and distinct local STs in clinPA of West and Central Africa. Furthermore, our data suggest that the spread of a ST could be either due to its antibiotic resistance or to features independent from the resistance to antibiotics.

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Outbreak of metallo- -lactamase VIM-2-positive strains of Pseudomonas aeruginosa in the Ivory Coast

Cited by 20 publications

References 10 publications

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide

Dual β-lactam combination therapy for multi-drug resistant Pseudomonas aeruginosa infection: enhanced efficacy in vivo and comparison with monotherapies of penicillin-binding protein inhibition

Population Structure of Clinical Pseudomonas aeruginosa from West and Central African Countries

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