Outcomes of vitrified–warmed day-4 embryos after day-3 cleavage-stage biopsy

Kahraman, Semra; Candan, Zafer Nihat

doi:10.1016/j.rbmo.2010.07.011

Cited by 8 publications

(11 citation statements)

References 24 publications

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“…These results were demonstrated for embryos that were biopsied either on day 3 or day 5 regardless of whether they were cryopreserved by slow freezing methods or by vitrification. [112][113][114][115][116][117] We at IHR/RGI 112 have been offering eSET to all patients <36 years since 2004, and when top-quality embryos are available for transfer, pregnancy rates were not compromised.…”

Section: Elective Single Embryo Transfer and Pgdmentioning

confidence: 99%

Clinical Management of in Vitro Fertilization with Preimplantation Genetic Diagnosis

Tur-Kaspa

2012

Semin Reprod Med

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Patients who undergo in vitro fertilization (IVF) because of preimplantation genetic diagnosis (PGD) require different clinical management than those who come in because of infertility alone. PGD adds a "fourth dimension" to the emotional aspect of a patients' assisted reproductive technology treatment. It significantly decreases the number of embryos available for transfer by 25 to 81%, and therefore ovarian stimulation for IVF with PGD should be tailored individually, taking into account patients' safety and estimated ovarian reserve. Recent studies showed that with increased number of oocytes retrieved, the higher the chance to have an embryo transfer and normal cryopreserved blastocysts. With adequate ovarian stimulation, there is no cutoff for the numbers of oocytes/embryos needed to start PGD with, especially for younger patients. Patient-friendly protocols, such as those based on gonadotropin-releasing hormone antagonist and vaginal progesterone support may be used. Elective single embryo transfer and blastocysts cryopreservation to avoid multiple pregnancies may be offered with PGD. The benefit of adding preimplantation genetic screening to IVF treatment is still controversial, and evidence-based data on 24-chromosome testing of polar bodies or trophectoderm is needed before it may be implemented into routine patient care.This review discusses the clinical management of IVF with PGD based on the best available data and my personal clinical experience as a reproductive specialist with >1000 IVF/intracytoplasmic sperm injection-PGD cycles. The information provided here will assist reproductive specialists, nurses, geneticists, genetic counselors, and embryologists to better counsel and treat couples who wish to conceive with a healthy child through IVF with PGD. It is time for PGD to be viewed as a modern modality of preventive medicine. As such, it should be incorporated into national health-care systems and be covered by medical insurance.

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Section: Elective Single Embryo Transfer and Pgdmentioning

confidence: 99%

Clinical Management of in Vitro Fertilization with Preimplantation Genetic Diagnosis

Tur-Kaspa

2012

Semin Reprod Med

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“…However, fortunately biopsied embryos may be efficiently vitrified, then result in high cryosurvival, and clinical pregnancy and implantation rates similar to those obtained in nonbiopsy cycles. [107][108][109][110][111] Moreover, embryo cryopreservation permits avoidance of the onset of ovarian hyperstimulation syndrome (OHSS) in patients at risk 112,113 and taking full advantage of the more appropriate status of the endometrium disengaged from any ovarian stimulation. In fact, the elevated levels of estradiol and progesterone during the follicular phase of ovarian stimulation may lead to morphological and biochemical alterations resulting in a less receptive endometrium, thus affecting ART success rates.…”

Section: Cleavage Stage Embryos and Blastocystsmentioning

confidence: 99%

Perspectives in Gamete and Embryo Cryopreservation

Rienzi¹,

Iussig²,

Dovere³

et al. 2018

Semin Reprod Med

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Cryopreserved gametes and embryos are a major feature of human-assisted reproduction and patient care services, accounting for an increasing number of births worldwide. Since the first success obtained using frozen human spermatozoa, cryopreservation technology has been successfully extended to include oocytes and embryos, in a variety of both medical and nonmedical indications. Over the years, the available procedures have become widely implemented and the increasing evidence of its efficacy has contributed to acceptance of the technology. Nevertheless, a gold standard protocol that would be universally shared by clinics has yet to be definitively established and, therefore, research into cryopreservation of gametes and embryos cannot be considered concluded. Moreover, much effort should be committed to the definition and resolution of safety issues, the establishment of automation, and investigations about the potentiality of immature germ cells or stem cells.

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“…The stimulation protocol was as outlined previously (Kahraman et al, 2010(Kahraman et al, , 2011. Gonadotrophin-releasing hormone (GnRH) antagonist and recombinant FSH (rFSH; Gonal-F®; Merck Serono, Turkey) were used for ovarian stimulation.…”

Section: Oocyte Retrieval Embryo Culture and Timelapse Imagingmentioning

confidence: 99%

Comparison of Blastocyst Development and Cycle Outcome in Patients with eSET Using Either Conventional or Time Lapse Incubators. A Prospective Study of Good Prognosis Patients

Kahraman

Çetinkaya

Pirkevi

et al. 2012

Journal of Reproductive and Stem Cell Biotechnology

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The aim of this study is to compare blastocyst development and cycle outcomes of good prognosis elective single embryo transfer patients using conventional or time lapse incubators. A randomized study was designed to evaluate embryo development until the blastocyst stage in either conventional or time-lapse incubators in good prognosis patients. Furthermore cycle outcomes of the patients were evaluated. The study involved 64 patients (33 for time-lapse and 31 for conventional incubation) in their first or second treatment cycle, with no recurrent spontaneous miscarriages, age<35 years, body mass index <28 kg/m2 and ≥8 oocytes retrieved. The primary outcome was the proportion of good and top quality blastocysts on day 5 and the secondary outcome was the implantation and pregnancy rates after elective single embryo transfer in good prognosis patients. The analysis performed revealed no statistical difference between both groups for blastocyst development, implantation and clinical pregnancy rates. In a selected good prognosis elective single embryo transfer population, similar rates of good and top quality blastocysts and comparable implantation and pregnancy rates were achieved. However, the stable culture conditions provided by time-lapse incubators and the use of morphokinetic parameters may allow the selection of the best available embryos with high developmental capacity, especially in poor prognosis patients.

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Outcomes of vitrified–warmed day-4 embryos after day-3 cleavage-stage biopsy

Cited by 8 publications

References 24 publications

Clinical Management of in Vitro Fertilization with Preimplantation Genetic Diagnosis

Clinical Management of in Vitro Fertilization with Preimplantation Genetic Diagnosis

Perspectives in Gamete and Embryo Cryopreservation

Comparison of Blastocyst Development and Cycle Outcome in Patients with eSET Using Either Conventional or Time Lapse Incubators. A Prospective Study of Good Prognosis Patients

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