Outer membrane vesicles as acellular vaccine against pertussis

Roberts, Roy; Moreno, Griselda; Bottero, Daniela; Gaillard, María Emilia; Fingermann, Matías; Graieb, Augusto; Rumbo, Martı́n; Hozbor, Daniela Flavia

doi:10.1016/j.vaccine.2008.07.004

Cited by 147 publications

(158 citation statements)

References 36 publications

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“…We also evaluated the kinetics of B. pertussis clearance by counting the numbers of bacteria recovered from the lungs of mice simultaneously treated with LPS and B. pertussis at different times posttreatment (2,6,24, and 48 h). The results obtained again showed significant differences between the animals challenged with B. pertussis alone and the LPS-plus-B.…”

Section: Resultsmentioning

confidence: 99%

“…All of the mouse procedures were performed in accordance with Argentine regulations. Inoculums (10 7 CFU/40 l) were prepared, and the mice were inoculated intranasally as previously described (24). For bacterial counting, the mice were sacrificed by cervical dislocation, and their lungs were removed and homogenized in 1 ml of sterile phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

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The Stimulated Innate Resistance Event in Bordetella pertussis Infection Is Dependent on Reactive Oxygen Species Production

et al. 2013

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The exacerbated induction of innate immune responses in airways can abrogate diverse lung infections by a phenomenon known as stimulated innate resistance (StIR). We recently demonstrated that the enhancement of innate response activation can efficiently impair Bordetella pertussis colonization in a Toll-like receptor 4 (TLR4)-dependent manner. The aim of this work was to further characterize the effect of lipopolysaccharide (LPS) on StIR and to identify the mechanisms that mediate this process. Our results showed that bacterial infection was completely abrogated in treated mice when the LPS of B. pertussis (1 g) was added before (48 h or 24 h), after (24 h), or simultaneously with the B. pertussis challenge (10 7 CFU). Moreover, we detected that LPS completely cleared bacterial infection as soon as 2 h posttreatment. This timing suggests that the observed StIR phenomenon should be mediated by fast-acting antimicrobial mechanisms. Although neutrophil recruitment was already evident at this time point, depletion assays using an anti-GR1 antibody showed that B. pertussis clearance was achieved even in the absence of neutrophils. To evaluate the possible role of free radicals in StIR, we performed animal assays using the antioxidant N-acetyl cysteine (NAC), which is known to inactivate oxidant species. NAC administration blocked the B. pertussis clearance induced by LPS. Nitrite concentrations were also increased in the LPS-treated mice; however, the inhibition of nitric oxide synthetases did not suppress the LPS-induced bacterial clearance. Taken together, our results show that reactive oxygen species (ROS) play an essential role in the TLR4-dependent innate clearance of B. pertussis.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Stimulated Innate Resistance Event in Bordetella pertussis Infection Is Dependent on Reactive Oxygen Species Production

et al. 2013

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“…OMVs from Salmonella enterica subsp. I serovar Typhimurium have been shown to stimulate both adaptive and innate im-mune responses, and OMVs from several species are immunogenic and protective in mouse models of infection (2,14,45,65). OMV-based intramuscularly delivered vaccines designed to protect against Neisseria meningitidis serogroup B infection have proved to be safe, immunogenic, and protective in human trials, as reviewed in reference 76. In 1977, it was found that subcutaneous immunization of mice with V. cholerae-derived vesicles protected their neonates from a lethal V. cholerae challenge (30).…”

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confidence: 99%

Mucosal Immunization with Vibrio cholerae Outer Membrane Vesicles Provides Maternal Protection Mediated by Antilipopolysaccharide Antibodies That Inhibit Bacterial Motility

et al. 2010

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Vibrio cholerae is the causative agent of cholera, a severe diarrheal disease that remains endemic in many parts of the world and can cause outbreaks wherever sanitation and clean water systems break down. Prevention of disease could be achieved through improved sanitation and clean water provision supported by vaccination. V. cholerae serogroup O1 is the major cause of cholera; O1 serotypes Inaba and Ogawa have similar disease burdens, while O139 is the only non-O1 serogroup to cause epidemics. We showed previously that immunization of adult female mice with purified V. cholerae outer membrane vesicles (OMVs) elicits an antibody response that protect neonates from oral V. cholerae challenge and that suckling from an immunized dam accounts for the majority of protection from V. cholerae colonization. Here we report that lipopolysaccharide (LPS) is the major OMV protective antigen. Mucosal immunization with OMVs from Inaba or Ogawa provides significant cross-serotype protection from V. cholerae colonization, although serotype-specific antigens are dominant. OMVs from O1 or O139 do not provide cross-serogroup protection, but by immunization with a mixture of O1 and O139 OMVs, cross-serogroup protection was achieved. Neonatal protection is not associated with significant bacterial death but may involve inhibition of motility, as antibodies from OMVimmunized mice inhibit V. cholerae motility in vitro, with trends that parallel in vivo protection. Motility assays also reveal that a higher antibody titer is required to immobilize O139 compared to O1, a phenotype that is O139 capsule dependent.

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“…OMVs from a variety of bacteria have broad effects on animal immune responses (reviewed in reference 89). Native and modified OMVs have been implemented as vaccines against pathogens, such as Salmonella enterica serovar Typhimurium and Vibrio cholerae, in animal models (26,90,91) and have reached clinical usage against Neisseria meningitidis (92)(93)(94). Due to the size of OMVs, visualizing them in situ has generally required electron microscopy of fixed material, but improvements in superresolution techniques hold promise for tracking OMVs, their contents, and their interactions in living environments.…”

Section: Implications Of Omvs As Materials Goods and Missiles In Micromentioning

confidence: 99%

Spheres of Hope, Packets of Doom: the Good and Bad of Outer Membrane Vesicles in Interspecies and Ecological Dynamics

Lynch

Alegado

2017

J Bacteriol

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Outer membrane vesicles (OMVs) are proteoliposome nanoparticles ubiquitously produced by Gram-negative bacteria. Typically bearing a composition similar to those of the outer membrane and periplasm of the cells from which they are derived, OMVs package an array of proteins, lipids, and nucleic acids. Once considered inconsequential by-products of bacterial growth, OMVs have since been demonstrated to mediate cellular stress relief, promote horizontal gene transfer and antimicrobial activity, and elicit metazoan inflammation. Recently, OMVs have gained appreciation as critical moderators of interorganismal dynamics. In this review, we focus on recent progress toward understanding the functions of OMVs with regard to symbiosis and ecological contexts, and we propose potential avenues for future OMV studies.

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Outer membrane vesicles as acellular vaccine against pertussis

Cited by 147 publications

References 36 publications

The Stimulated Innate Resistance Event in Bordetella pertussis Infection Is Dependent on Reactive Oxygen Species Production

The Stimulated Innate Resistance Event in Bordetella pertussis Infection Is Dependent on Reactive Oxygen Species Production

Mucosal Immunization with Vibrio cholerae Outer Membrane Vesicles Provides Maternal Protection Mediated by Antilipopolysaccharide Antibodies That Inhibit Bacterial Motility

Spheres of Hope, Packets of Doom: the Good and Bad of Outer Membrane Vesicles in Interspecies and Ecological Dynamics

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