Outlook in the application of<i>Chlamydomonas reinhardtii</i>chloroplast as a platform for recombinant protein production

Shamriz, Shabnam; Ofoghi, Hamideh

doi:10.1080/02648725.2017.1307673

Cited by 27 publications

(16 citation statements)

References 61 publications

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“…C. reinhardtii has gained significant applications in the biotechnological industry, becoming a platform for obtaining natural products of very high commercial value. The chloroplast of C. reinhardtii could be used as a platform for recombinant protein production (Shamriz & Ofoghi, 2016). Moreover, C. reinhardtii has applications in the production of biohydrogen (Xu, Fan, & Wang, 2019), which could potentially be used in hydrogen fuel cells.…”

Section: Introductionmentioning

confidence: 99%

Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment

et al. 2021

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Ongoing technological advancements continually increase the demand for energy. Among various types of energy harvesting systems, biologically based systems have been an area of increasing interest for the past couple of decades. Such systems provide clean, safe power solutions, mainly for low-and ultralow-power applications. The microphotosynthetic power cell (μPSC) is one such system that make use of photosynthetic living cells or organisms to generate power. For strong performance, μPSC technology, because of its interdisciplinary nature, requires optimal engineering of both electrochemical cell design and the culture conditions of the photosynthetic microorganisms. We present here a simple, economical culture method for the photosynthetic microorganism Chlamydomonas reinhardtii suitable for the application of this biologically based power system in any geographical location. This article provides a series of protocols for preparing materials and culture medium designed to facilitate the culture of a suitable C. reinhardtii strain even in a non-biological laboratory. Possible challenges and methods to overcome them are also discussed. Cultured C. reinhardtii perform sufficiently well that they have already been successfully utilized to generate power from a μPSC, generating a peak power of 200 μW from just 2 ml of exponential-phase algal culture in a μPSC with an active electrode surface area of 4.84 cm 2 . The μPSC thus has potentially broad applications in low-and ultra-low-power devices and sensors.

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Section: Introductionmentioning

confidence: 99%

Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment

et al. 2021

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“…Microalgae such as the unicellular green alga C. reinhardtii have many advantages that make them ideal for recombinant protein production [ 47 , 48 ]. However, low yield and inefficient downstream processing are the main obstacles to engineering algae strains for industrialisation [ 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpressing CrePAPS Polyadenylate Activity Enhances Protein Translation and Accumulation in Chlamydomonas reinhardtii

Wang

Zhuang

et al. 2022

Marine Drugs

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The alga Chlamydomonas reinhardtii is a potential platform for recombinant protein expression in the future due to various advantages. Dozens of C. reinhardtii strains producing genetically engineered recombinant therapeutic protein have been reported. However, owing to extremely low protein expression efficiency, none have been applied for industrial purposes. Improving protein expression efficiency at the molecular level is, therefore, a priority. The 3′-end poly(A) tail of mRNAs is strongly correlated with mRNA transcription and protein translation efficiency. In this study, we identified a canonical C. reinhardtii poly(A) polymerase (CrePAPS), verified its polyadenylate activity, generated a series of overexpressing transformants, and performed proteomic analysis. Proteomic results demonstrated that overexpressing CrePAPS promoted ribosomal assembly and enhanced protein accumulation. The accelerated translation was further verified by increased crude and dissolved protein content detected by Kjeldahl and bicinchoninic acid (BCA) assay approaches. The findings provide a novel direction in which to exploit photosynthetic green algae as a recombinant protein expression platform.

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“…As one of the most studied microalgae for genetic engineering, C. reinhardtii is widely used as an algal recombinant expression platform for biomedical applications. 34 A novel approach using transgenic C. reinhardtii was developed to produce oxygen and proangiogenic growth factors such as human vascular endothelial growth factor A (hVEGF-165) to promote wound healing in biomedical settings. 35 The study identified the optimal combination of C. reinhardtii and protein expression elements to produce recombination proteins in a high-efficiency model.…”

Section: Eukaryote Microalgaementioning

confidence: 99%

How Microalgae is Effective in Oxygen Deficiency Aggravated Diseases? A Comprehensive Review of Literature

Cui¹,

Su²,

Wei³

et al. 2022

IJN

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Hypoxia can aggravate the conditions of many oxygen-deficiency-aggravated diseases (ODAD), such as cancer, ischemic heart disease, and chronic wounds. Photosynthetic microalgae can alleviate the hepatotoxicity of the local microenvironment by producing oxygen. In addition, microalgae extracts have antitumor, anti-inflammatory, antibacterial, and antioxidant effects. These properties make them attractive candidates for developing methods to treat ODAD. Although researchers have exploited the advantages of microalgae and developed a variety of microalgae-based biomaterials to treat ODAD, a comprehensive review of this topic has not been presented previously. Therefore, in this review, we summarize the development and progress made in the field of developing microalgae-based biomaterials toward the treatment of ODAD. The challenges and prospects of this field are also discussed.

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Outlook in the application ofChlamydomonas reinhardtiichloroplast as a platform for recombinant protein production

Cited by 27 publications

References 61 publications

Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment

Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment

Overexpressing CrePAPS Polyadenylate Activity Enhances Protein Translation and Accumulation in Chlamydomonas reinhardtii

How Microalgae is Effective in Oxygen Deficiency Aggravated Diseases? A Comprehensive Review of Literature

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