Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment

Abstract

Ongoing technological advancements continually increase the demand for energy. Among various types of energy harvesting systems, biologically based systems have been an area of increasing interest for the past couple of decades. Such systems provide clean, safe power solutions, mainly for low-and ultralow-power applications. The microphotosynthetic power cell (μPSC) is one such system that make use of photosynthetic living cells or organisms to generate power. For strong performance, μPSC technology, because of its interdisciplinary nature, requires optimal engineering of both electrochemical cell design and the culture conditions of the photosynthetic microorganisms. We present here a simple, economical culture method for the photosynthetic microorganism Chlamydomonas reinhardtii suitable for the application of this biologically based power system in any geographical location. This article provides a series of protocols for preparing materials and culture medium designed to facilitate the culture of a suitable C. reinhardtii strain even in a non-biological laboratory. Possible challenges and methods to overcome them are also discussed. Cultured C. reinhardtii perform sufficiently well that they have already been successfully utilized to generate power from a μPSC, generating a peak power of 200 μW from just 2 ml of exponential-phase algal culture in a μPSC with an active electrode surface area of 4.84 cm 2 . The μPSC thus has potentially broad applications in low-and ultra-low-power devices and sensors.

Arraying of microphotosynthetic power cells for enhanced power output

“…Cells were grown in the presence of constant light illumination of 20 µEm −2 s −1 with white fluorescent tubes (Philips). Erlenmeyer flasks containing algal culture were cultured on an orbital shaker at 90 RPM 33 . The algal culture grown to the exponential phase (48 hours) (Fig.…”

Hyperspectral Analysis on the Photosynthetic PS-II system: Study on Chlamydomonas reinhardtii

“…Due to the strain's superior growth factor, three weeks of growth yield favourable colony density. These harvested strains are suspended in pH-neutral TAP (Tris-acetate-phosphate medium) nutrient media for three days to reach the exponential growth phase of multiplication before the commencement of the study [10]. The algal strains are monitored at 24-hour intervals for any contamination and abnormalities.…”