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This paper presents a new approach to studying the nano-sac or nano-site of photosystem components in photosynthesis. Instead of analyzing bulk colonies of cells or using complicated biological pigment isolation procedures, hyperspectral characterization is used to investigate individual algal cells. This method eliminates the need for bulk characterization and allows for a more precise analysis of the PS-II nano-sac/nano-sites. The results of the hyperspectral studies using Cytoviva™ are presented in this paper.