2021
DOI: 10.3390/cells10082126
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Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro

Abstract: Ovarian failure is the most common cause of infertility. Although numerous strategies have been proposed, a definitive solution for recovering ovarian functions and restoring fertility is currently unavailable. One innovative alternative may be represented by the development of an “artificial ovary” that could be transplanted in patients for re-establishing reproductive activities. Here, we describe a novel approach for successful repopulation of decellularized ovarian bioscaffolds in vitro. Porcine whole ovar… Show more

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Cited by 23 publications
(16 citation statements)
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“…When a whole-organ decellularization protocol, previously developed in our laboratory, [ 23 , 24 , 25 ] was applied to aged ovaries, we were able to derive 3D ECM-based biological scaffolds that represent a viable alternative to the use of synthetic matrix such as hydrogels [ 49 ]. The decellularized scaffolds preserve all the changes that are described above and that are distinctive of the senescent organ.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…When a whole-organ decellularization protocol, previously developed in our laboratory, [ 23 , 24 , 25 ] was applied to aged ovaries, we were able to derive 3D ECM-based biological scaffolds that represent a viable alternative to the use of synthetic matrix such as hydrogels [ 49 ]. The decellularized scaffolds preserve all the changes that are described above and that are distinctive of the senescent organ.…”
Section: Discussionmentioning
confidence: 99%
“…Young and aged whole-ovaries were decellularized according to the protocol previously developed in our laboratory [ 23 , 24 , 25 ]. Briefly, the entire organs were frozen at –80 °C for at least 24 h. They were then thawed at 37 °C in a water bath for 30 min, followed by an incubation with 0.5% sodium dodecyl sulfate (SDS; Bio-Rad, Milan, Italy) in deionized water (dd-H 2 O) for 3 h. Ovaries were then treated overnight with 1% Triton X-100 (Sigma, Milan, Italy) in dd-H 2 O, extensively washed in dd-H 2 O for 9 h, and, subsequently, immersed in 2% deoxycholate in dd-H 2 O (Sigma, Milan, Italy) for 12 h. Lastly, decellularized whole-ovaries were washed in dd-H 2 O for 6 h, with changes every 2 h. All steps were carried out at room temperature using an orbital shaker at 200 rpm.…”
Section: Methodsmentioning
confidence: 99%
“…18 ovaries from 6-month ( n = 9, Young) and 5 years old ( n = 9, Aged) swine were collected at the local abattoir and subjected to whole-organ decellularization protocol [ 2 , 30 32 ]. Briefly, organs were frozen at − 80 °C for at least 24 h, thawed in a 37 °C water bath for 30 min, treated with 0.5% sodium dodecyl sulfate (SDS; Bio-Rad) for 3 h, and immersed overnight in 1% Triton X-100 (Sigma-Aldrich).…”
Section: Methodsmentioning
confidence: 99%
“…Accordingly, we decellularized ovaries using combined multiple decellularization strategies (mechanical, chemical and biological reagents). Other research groups have also used combined methods, among which SDS, Triton X-100, and SDC were the most frequently used [ 15 , 19 ]. Other reagents such as sodium lauryl ester sulfate, NaOH, and propanol-2 were also used to decellularize ovaries [ 12 , 20 , 21 ].…”
Section: Discussionmentioning
confidence: 99%