Ovarian expression and localization of a vitellogenin receptor with eight ligand binding repeats in the cutthroat trout (Oncorhynchus clarki)

Vitellogenesis and choriogenesis in fishes

“…This finding is in agreement with our experimental results, which biochemically demonstrated the presence of a binding region on Lv that binds to the Lr8-type Vtg receptor [65]. Moreover, Mizuta et al [122] prepared a specific antibody to a recombinant homologue of the Lr8-type Vtg receptor in cutthroat trout Oncorhynchus clarki and immunobiochemically proved that an approximately 100 kDa receptor protein was the translation product of the gene encoding the Lr8-type Vtg receptor. Furthermore, immunohistochemical analysis using the same antibody demonstrated that the Lr8-type Vtg receptor protein is mainly located at the periphery of oocyte membranes within the ovary, supporting the hypothesis that the Lr8-type receptor binds to Vtg at this same site to be taken up into oocytes.…”

Two novel linkage SNPs of VLDLR gene intron 11 are associated with laying traits in two quail populations

“…The variation is displayed by genetic differences between individuals, families and populations within a given species (Groeneveld et al, 2010). Many studies reported that the VLDLR gene was associated with reproduction in oviparous species (Agulleiro et al, 2007;Han et al, 2009;Wang et al, 2011;Roth and Khalaila, 2012;Mizuta et al, 2013). In this study, the VLDLR gene was selected as a candidate gene to investigate associations of gene polymorphisms with some laying traits in two quail populations.…”

How do eggs get fat? Insights into ovarian fatty acid accumulation in the shortfinned eel, Anguilla australis

“…As previously reported, Mizuta et al (2011Mizuta et al ( , 2013) generated antisera against recombinant proteins that encompassed the ligand binding repeats of the vtgr or LDLr of cutthroat trout (Oncorhynchus clarkii). The specificity of the resulting antisera and their suitability for use on eel was examined by Western blotting.…”