Ovarian Innervation Coupling With Vascularity: The Role of Electro-Acupuncture in Follicular Maturation in a Rat Model of Polycystic Ovary Syndrome

Background. Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women of reproductive age. As a widely used complementary and alternative therapy, acupuncture is increasingly used to treat PCOS. However, the effect of acupuncture in treating PCOS is uncertain, and the mechanisms are unclear. This systematic review aims to determine the efficacy of acupuncture on PCOS in animal preclinical models. Methods. Experimental animal studies of acupuncture in PCOS animal models were searched in PubMed, Web of Science, China National Knowledge Infrastructure, and the Chinese Science and Technology Periodical Database from inception to December 2020. The risk of bias was assessed using the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) risk of bias tool. Results. A total of 358 studies were screened based on the title and abstract, and 31 studies were included. A total of 722 animals were involved, and all studies used either Wistar rats or SD rats. Twenty-six studies used electroacupuncture, 9 studies used manual acupuncture, and 5 of them employed both electroacupuncture and manual acupuncture. A total of 22 acupoints were involved; 7 studies followed the modern acupuncture pattern, and the rest followed classic acupuncture theory. Conclusions. The present review summarizes the current evidence of the effects of acupuncture on PCOS in animal models. Unfortunately, we could not draw a definite conclusion due to the methodological weakness of the included studies and the high heterogeneity. Well-designed studies are needed in the future to fill this gap.

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“…A total of 31 studies were included [ 13 , 14 , 19 – 46 ]. Thirteen studies were published in English, and 18 were published in Chinese.…”

Section: Resultsmentioning

confidence: 99%

“…It has been newly demonstrated that ovarian innervation likely plays an important role in folliculogenesis, and EA might restore PCOS pathophysiology by regulating ovarian innervation, at least partially mediated through the superior ovarian nerve. The effect of EA is based on the integrity of the nervous system [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Acupuncture on Polycystic Ovary Syndrome in Animal Models: A Systematic Review

Zhang

Gao

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…In a recent 3D study of innervation in postnatal and adult mouse ovaries using CUBIC-clearing Tong et al (2020) showed that each follicle is innervated by a single neural fiber; furthermore, peripheral nerve branching around follicles increases at later stages of folliculogenesis ( Figure 1D ). They further showed that PMSG-induced follicular growth contributed to a visible increase in TH-positive neuronal fibers in the ovary within 48-h. Tracing the long and circuitous routes of nerve fibers could not be accomplished through traditional histology, but is ripe for 3D techniques.…”

Section: Clearing Techniques and Quantitative Approaches Used For Assessment Of Ovaries In 3dmentioning

confidence: 92%

“…EA treatment partially rescued the PCOS-like phenotype by increasing total vascular area and volume, particularly in mature follicles, suggesting that elevated blood flow allows mature follicles to receive signals required for ovulation. The role of innervation in PCOS pathology and its possible treatment via EA therapy were also studied in rat ovaries using CUBIC clearing and 3D analysis ( Tong et al, 2020 ). EA treatment reversed the increased innervation in the ovarian stroma and the diminished neuronal network around individual follicles in PCOS-like ovaries.…”

Section: D Analysis Of the Ovary In Different Speciesmentioning

confidence: 99%

Ovary Development: Insights From a Three-Dimensional Imaging Revolution

Soygur

Laird

2021

Front. Cell Dev. Biol.

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The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodeling during each hormonal cycle. Overcoming the limitations of traditional histology, recent advances in optical tissue clearing and three-dimensional (3D) visualization offer an advanced platform to explore the architecture of intact organs at a single cell level and reveal new relationships and levels of organization. Here we summarize the development and function of ovarian compartments that have been delineated by conventional two-dimensional (2D) methods and the limits of what can be learned by these approaches. We compare types of optical tissue clearing, 3D analysis technologies, and their application to the mammalian ovary. We discuss how 3D modeling of the ovary has extended our knowledge and propose future directions to unravel ovarian structure toward therapeutic applications for ovarian disease and extending female reproductive lifespan.

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“…Also, experiments on PCOS animal models showed that electrical acupuncture (EA) ameliorated insulin resistance and mitochondrial dysfunction by promoting autophagy [11]. EA regulates ovarian innervations to increase the numbers of preovulatory follicles in PCOS-like rats [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Acupuncture on Polycystic Ovary Syndrome in Animal Models: Study Protocol for a Systematic Review and Meta-Analysis

Gao

et al. 2021

Preprint

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Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women of reproductive age. As a widely used complementary and alternative therapy, acupuncture is increasingly used to treat PCOS. However, the effect of acupuncture in treating PCOS is uncertain and the mechanisms are unclear. This systematic review aims to determine the efficacy of acupuncture on PCOS in animal preclinical models.Methods: We will search the following databases: PubMed, Web of Science, China National Knowledge Infrastructure and Chinese Science and Technology Periodical Database. We will only include animal experiments of acupuncture in treating PCOS. The primary outcome will be homeostatic model assessment- insulin resistance. The risk of bias will be assessed using the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) risk of bias tool. Confidence in the cumulative evidence will be assessed using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. All meta-analyses will be conducted using Review Manager 5.4. Discussion: To the best of our knowledge, the use of acupuncture in treating PCOS has not yet been systematically reviewed in animal models. The evidence generated from this systematic review and meta-analysis could benefit future researches. Systematic review registration: OSF (Registration DOI: 10.17605/OSF.IO/FNM37)

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Ovarian Innervation Coupling With Vascularity: The Role of Electro-Acupuncture in Follicular Maturation in a Rat Model of Polycystic Ovary Syndrome

Cited by 13 publications

References 52 publications

Effect of Acupuncture on Polycystic Ovary Syndrome in Animal Models: A Systematic Review

Effect of Acupuncture on Polycystic Ovary Syndrome in Animal Models: A Systematic Review

Ovary Development: Insights From a Three-Dimensional Imaging Revolution

Effect of Acupuncture on Polycystic Ovary Syndrome in Animal Models: Study Protocol for a Systematic Review and Meta-Analysis

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