Ovarian steroids, oxytocin, and tumor necrosis factor modulate equine oviduct function

Pinto‐Bravo, Pedro; Galvão, António; Rebordão, Maria Rosa; Amaral, Ana; Ramilo, David W.; Silva, E.; Szóstek‐Mioduchowska, Anna; Alexandre‐Pires, Graça; Costa, R. Roberto da; Skarzynski, Dariusz J.; Ferreira‐Dias, Graça

doi:10.1016/j.domaniend.2017.06.005

Cited by 7 publications

(6 citation statements)

References 58 publications

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“…Prostaglandins (PG) are also present in oviductal secretions. Both PGE 2 and PGF 2α were reported in tubal fluid and in culture media conditioned by oviductal explants or epithelial cells in pigs, horse, and cattle [56][57][58]. The expression of numerous enzymes involved in prostaglandin synthesis has also been reported in the oviduct of various mammals [56,57,59].…”

Section: Hormonesmentioning

confidence: 96%

“…Both PGE 2 and PGF 2α were reported in tubal fluid and in culture media conditioned by oviductal explants or epithelial cells in pigs, horse, and cattle [56][57][58]. The expression of numerous enzymes involved in prostaglandin synthesis has also been reported in the oviduct of various mammals [56,57,59]. Prostaglandins, together with steroid hormones, may participate in the optimal transport of gametes and embryo through their modulating effects on both the muscular contractility and ciliary beat frequency in the oviduct (for review, see the work of [60]).…”

Section: Hormonesmentioning

confidence: 99%

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Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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The oviductal fluid is the first environment experienced by mammalian embryos at the very beginning of life. However, it has long been believed that the oviductal environment was not essential for proper embryonic development. Successful establishment of in vitro embryo production techniques (which completely bypass the oviduct) have reinforced this idea. Yet, it became evident that in vitro produced embryos differ markedly from their in vivo counterparts, and these differences are associated with lower pregnancy outcomes and more health issues after birth. Nowadays, researchers consider the oviduct as the most suitable microenvironment for early embryonic development and a substantial effort is made to understand its dynamic, species-specific functions. In this review, we touch on the origin and molecular components of the oviductal fluid in mammals, where recent progress has been made thanks to the wider use of mass spectrometry techniques. Some of the factors and processes known to regulate oviductal secretions, including the embryo itself, as well as ovulation, insemination, endogenous and exogenous hormones, and metabolic and heat stress, are summarized. Special emphasis is laid on farm animals because, owing to the availability of sample material and the economic importance of fertility in livestock husbandry, a large part of the work on this topic has been carried out in domestic animals used for dairy and/or meat production.

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Section: Hormonesmentioning

confidence: 96%

Section: Hormonesmentioning

confidence: 99%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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“…The changes are mainly dependent on ovarian steroids, which affect the oviduct epithelium and secretion of oviduct fluid. Among these, progesterone and 17β-oestradiol exert their actions on specific receptors to induce morphological changes during the oestrous cycles (Pinto-Bravo et al, 2017), including epithelial degeneration and proliferation during the oestrous cycle. Both extrinsic (Fas/Fasl) (Bergqvist et al, 2005a,b;Tienthai et al, 2011) and intrinsic (Bcl-2/ Bax) (Christine et al, 2006;Ulbrich et al, 2006) pathways are involved in the remodelling of the ovarian epithelium.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of apoptosis‐related gene expression during follicular development in yak

Zhang

Basang

Chang

et al. 2021

Animal Physiology Nutrition

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The potential reproduction power of domestic animals is limited by a complicated follicular atresia process. P53, caspase-9 (Casp9), Bax, Bcl-2 and Fas play a crucial role in the ovarian mitochondrion-dependent apoptosis and death receptor pathway. In accordance with this study, the expression levels of Casp9, Bax, Bcl-2 and Fas were analysed in ovaries and oviducts of yak by immunohistochemistry (IHC). P53 and the above in ovarian granulosa cells (GCs) from atretic (3-6 mm) to healthy follicles (6-8 mm) and in oviducts were examined from the luteal phase to the follicular phase during the oestrous circle by Western blot (WB) and real-time PCR (RT-PCR). Results demonstrated that typical classic apoptotic factors Casp9, Bax, Bcl-2 and Fas were expressed in the cytoplasm and zonal pellucida of oocytes, primordial follicles, primary follicles, ovarian surface epithelium, ovarian GCs, granular lutein cells, surface epithelia in oviduct uterotubal junction and oviduct ampulla during the luteal phase. RT-PCR and WB revealed that P53 and Fas significantly increased in GCs of atretic follicles.P53 and Casp9 increased in oviduct epithelium during the luteal phase, but Fas was unchanged. A contrary tendency was noted in Bcl-2 and Bax expression. Overall, P53 and Fas play an essential role in inducing GC apoptosis, and Bax, Bcl-2, Casp9 and P53 are involved in oviduct epithelial regeneration in yak.

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“…In addition, visualization of 28S and 18S rRNA bands after electrophoresis through a 1.5% agarose gel and red staining (41003; Biotium, Hayward, CA, USA) was accomplished. Reverse transcription was performed with the Reverse Transcriptase Superscript III enzyme (18080093; Invitrogen, GIBCO BRL, Carlsbad, CA, USA) from 400 µg total RNA in a 20 µL reaction volume using oligo(dT) primer (27-7858-01; GE Healthcare, Buckinghamshire, UK), as previously described [41,42]. Specific primers for FGF1, FGF2, FGFR1, FGFR2, VEGF and KDR, as well as reference genes (Table 1) were designed using Primer3 Software and confirmed with Primer Express ® (Applied Biosystems, Foster City, CA, USA).…”

Section: Experiments 2 231 Gene Transcription Analysismentioning

confidence: 99%

“…Western blot analyses for relative protein abundance were performed as previously described [41] on the infundibulum, ampulla and isthmus from 15 mares in different phases of the estrous cycle (FP, n = 5; ELP, n = 5; MLP, n = 5). Briefly, oviduct samples were minced and placed in ice-cold RIPA buffer (50 mM Tris-HCl, pH 7.4, 50 mM EDTA, 150 mM NaCl and 1% Triton X-100) with a protease inhibitor cocktail (Complete Mini Protease Inhibitor Cocktail Tablets, 1 tablet per 10 mL of buffer; Roche) and homogenized on ice.…”

Section: Relative Protein Abundancementioning

confidence: 99%

Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct

Pinto‐Bravo

Rebordão

Amaral

et al. 2021

Animals

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The oviduct presents the ideal conditions for fertilization and early embryonic development. In this study, (i) vascularization pattern; (ii) microvascular density; (iii) transcripts of angiogenic factors (FGF1, FGF2, VEGF) and their receptors—FGFR1, FGFR2, KDR, respectively, and (iv) the relative protein abundance of those receptors were assessed in cyclic mares’ oviducts. The oviductal artery, arterioles and their ramifications, viewed by means of vascular injection-corrosion, differed in the infundibulum, ampulla and isthmus. The isthmus, immunostained with CD31, presented the largest vascular area and the highest number of vascular structures in the follicular phase. Transcripts (qPCR) and relative protein abundance (Western blot) of angiogenic factors fibroblast growth factor 1 (FGF1) and 2 (FGF2) and vascular endothelial growth factor (VEGF), and their respective receptors (FGFR1, FGFR2, VEGFR2 = KDR), were present in all oviduct portions throughout the estrous cycle. Upregulation of the transcripts of angiogenic receptors FGF1 and FGFR1 in the ampulla and isthmus and of FGF2 and KDR in the isthmus were noted. Furthermore, in the isthmus, the relative protein abundance of FGFR1 and KDR was the highest. This study shows that the equine oviduct presents differences in microvascular density in its three portions. The angiogenic factors VEGF, FGF1, FGF2 and their respective receptors are expressed in all studied regions of the mare oviduct, in agreement with microvascular patterns.

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Ovarian steroids, oxytocin, and tumor necrosis factor modulate equine oviduct function

Cited by 7 publications

References 58 publications

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Dynamics of apoptosis‐related gene expression during follicular development in yak

Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct

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